钝顶螺旋藻质粒的电镜观察及杂交研究

于１９９２年６月－１９９３年４月，对钝顶螺旋藻质粒进行透射电镜观察及分子杂交研究。结果表明，利用层分子膜展开技术，能够观察到超螺旋以及闭合环形的质粒图象；Ｓ６质粒大小的平均值约为２．０ｋｂ，与电泳检测的结果稍有出科。斑点杂交结果表明，不同形态藻株Ｓ６、Ｆ３、Ｆ３弯与Ｆ３直质粒之间具有一定同源性，与染色体ＤＮＡ之间均显示同源性。对钝顶螺旋藻质粒的进一步研究将推动其基因工程育种的开展。     

胶州湾口内初级生产力的周年定点观测

于１９８３年１１月－１９８５年１１月利用黑白瓶测氧法研究了胶州湾东南部的初级生产力及其与各环境因子的关系。结果表明，调查区内初级生产力的变化范围是０．１０－２．８２ｇ／（ｍ＾２．ｄ）碳，年初级生产量的平均是２０６ｇ／ｍ＾２碳，夏半年（５－１０月）的初级生产量平均占全年生产量的７３．４５％，浮游植物的光合作用对有效太阳辐射能的平均利用率是０．６４％，初级生产力与水温，日太阳辐射能等均呈明显的正相磁，     

钝顶螺旋藻藻胆体的简易制备及褐藻酸钠对其稳定性的保护作用

以钝顶螺旋藻为材料，用改进的简易方法分离得到完整藻胆体，室温最大荧光发射峰位于６８１ｎｍ，加水解离后最大荧光发射蓝移到６５５ｎｍ，Ｆ６８０有两个峰，分别位于６３８ｎｍ和６５０ｎｍ，从而表明藻胆是完整的。用４３６ｎｍ波长激发，没有出现Ｃｈｌａ的特征荧光峰，Ｆ７２０的荧光激发光谱，也没有出现Ｃｈｌａ的峰，表明藻胆体溶液中不含Ｃｈｌａ。在藻胆体溶液中加入１％的褐藻酸钠，室温下保存１５ｄ后，光谱特性没有变     

西沙群岛原绿藻的超微构造研究

于1981年3月在西沙群岛永兴岛采集原绿藻标本。用光镜和电镜对所采原绿藻进行研究。结果表明,原绿藻为简单的原核、单细胞藻类,在同一生境的相同宿主体表有4种不同的细胞构造类型：第1种类型,中央大“液泡”,类囊体单条、平行分散于周围区的细胞质中;第2种类型,中央是大“液泡”,周围区内类囊体多,并形成垛叠;第3种类型,许多小泡充满细胞的大部分,类囊体穿插于小泡之间;第4种类型,有中央大“液泡”,还有许多小泡分散在细胞周围区。上述分类以类囊体和“液泡”的结构和分布为主要分类依据,反映出不同结构的进化意义。表明中国西沙群岛有丰富的原绿藻资源。     

EXCITATION ENERGY TRANSFER IN VITRO BETWEEN PHYCOBILIPROTEINS AND THYLAKOID PHOTOSYSTEM Ⅱ OF HIGHER PLANTS

The excitation energy transfer from phycobiliproteins to thylakoid PSII of higher plants was investigated. When incubated with spinach thylakoids, phycobiliproteins isolated from red and blue- green algae transferred light energy absorbed to spinach PSII. The efficiency of energy transfer was dependent on the kind of phycobiliproteins used. If spinach thylakoids were replaced by the thylakoids of Brassica chinensis, R phycoerythin or C- phycocyanin did not transfer their excitation energy to PSII of Brassica chinensis unless allophycocyanin was present.     

A preliminary study on Typhoon Tim induced resonance at Hualien Harbor,Taiwan

New analysis of wave records at Hualien Harbor during Typhoon Tim in 1994 reveals that for certain wave periods, the ratios of measured wave heights among three available wave stations inside the harbor are unique and roughly remain the same during the severest period of resonance. Since there is no incident infragravity wave (period from 80 to 220 s) information at offshore boundaries, these unique ratios become the surrogate of background truth for checking the performance of numerical simulations. A new simulation effort using a large (45 × 17 km) modeling domain, high-resolution (20 × 20 m) grid and the RIDE wave transformation model were conducted to simulate the observed responses. Comparison of the modeling results with the observations showed reasonable agreement. Additional model studies using ideal bathymetries with the same grid domain and resolution were also conducted to help interpret the prototype modeling results. The effects of two types of commonly used remedies were first examined by using the ideal bathymetry, and then, the prototype bathymetry. The results demonstrated that a single 1-km long, shore-parallel breakwater could significantly reduce the resonance. The results of using three shore-parallel breakwaters, however, are no better. More studies to identify the optimum design associated with the shore-parallel breakwater (location, length, etc.) are necessary for the optimum reduction of resonance at Hualien Harbor.     

海带愈伤组织的高效率诱导

于１９９４年１月和１１月在青岛太平角和荣成海带育苗场分别采集海带成熟孢子体和幼孢子体,采用培养基诱导方法,以４种培养基诱导海带愈伤组织的效果,成熟海带孢子体的不同部位在ＭＳ固体增富２基中形成愈 组织的能力进行了比较;研究了添另不同成分的ＭＳ固体增富２基对海带愈伤组织的影响,并比较了４种除菌方法的效果。结果表明,ＰＥＳＩ固体２基和ＭＳ固体在是诱导海带愈伤组织的理想培养基,其诱导率分别为７５．５％和６