CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Technique for Controlling Spread of Limnotic Oncomelania

Schistosomiasis is a parasitic disease mostly found in areas along the Changjiang River of China. The disease is spread solely through an intermediary named oncomelania, so its spread of schistosomiasis can be controlled by properly designing water intakes which prevent oncomelania from entering farming land or residential areas. This paper reports a successful design process and a new oncomelania-free intake device. The design of the new intake is based on a sound research program in which extensive experimental studies were carried out to gain knowledge of oncomelania eco-hydraulic behavior and detailed flow field information through CFD simulation.     

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.     

APPLICATION OF GEOGRAPHICAL PARAMETER DATABASE TO ESTABLISHMENT OF UNIT POPULATION DATABASE

Now GIS is turning into a good tool in handling geographical, economical, and population data, so we can obtain more and more information from these data. On the other hand, in some cases, for a calamity, such as hurricane, earthquake, flood, drought etc., or a decision-making, such as setting up a broadcasting transmitter, building a chemical plant etc., we have to evaluate the total population in the region influenced by a calamity or a project. In this paper, a method is put forward to evaluate the population in such special region. Through exploring the correlation of geographical parameters and the distribution of people in the same region by means of quantitative analysis and qualitative analysis, unit population database (1km× 1km) is established. In this way, estimating the number of people in a special region is capable by adding up the population in every grid involved in this region boundary. The geographical parameters are obtained from topographic database and DEM database on the scale of     

EMERGY ANALYSIS OF GRAIN PRODUCTION SYSTEM IN JIANGSU AND SHAANXI PROVINCES

Efficiency and sustainability of grain production are now important in China. In this study, the grain production systems of Jiangsu and Shaanxi Provinces were compared, to analyze their efficiency and sustainability in terms of utilization of natural resources, inputs of purchased energy and materials, and outputs. Flows of energy and materials between environment and human society were identified, and the natural and human work involved in generating inputs as materials or energy were valued in terms of equivalent amount of solar energy required for their production using emergy method. The results showed environmental resources were continually playing a less important role in the systems, when inorganic subsidiary emergy inputs increased drastically while organic ones decreased or increased little. Deterioration of input emergy structure affected the systems‘ efficiency and sustainability, resulting in emergy investment ratios and environmental loading ratios increasing while yield ratios and sustainability indices decreasing. In general, efficiency and sustainability of grain production in Jiangsu are worse than those in Shaanxi. This analysis also suggested that inorganic subsidiary emergy should be introduced properly, and peasants in Jiangsu should utilize natural conditions wisely while those in Shaanxi pay enough attention to soil and surface water conservation.     

射电源0420—014,1334—127和2345—167中的视超光速运动

本文叙述南天区 5GHzVLBI普查中新发现的视超光速射电源 ,0 4 2 0— 0 1 4,1 334—1 2 7和 2 345— 1 67,这些结果尚待进一步的观测加以证实     

CORRESPONDENCE ANALYSIS ON IMAGES OF JIANGXI PROVINCE AS A TOURIST DESTINATION

1IN T R O D U C T IO NAn image isa setofbelief,s ideasand impressionsthatapersonholdsaboutan object(KOTLER,1991).Similar-lydefinitionistheimage of a destinationas thesum ofbelief,sideasand impressionsthatpeoplehave ofa placeor destination(CROMPTON,1979;KO…     

Petroleum Hydrocarbon Distribution Features in Water and Sediment off Fujian Shore

This study on the distribution features of petroleum hydrocarbon in water and sedi-ment off the Fujian shore using data obtained from the baseline research on oceanic contamination in Fujian showed that:during the research preiod,petroleum hydrocarbon concentrations in water varied from 5.77μg/L to 37.28μg/L,averaged 14.48 μg/L;was lower in the wet seasson than in the dry season;and was highest in the Minjiang Estuary and Jiulong Estuary in both seasons ,The petorleum hydrocarbon concentrations in shore sediment varied from 14.48 mg/kg to 784.36 mg/kg,averaged 133.3 mg/kg ,and was closely related to sediment types (granularity).     

Is Precipitation the Dominant Controlling Factor of High Inorganic Nitrogen Content in the Changjiang River and Its Mouth？

The main reasons for the high content of inorganic N and its increase by several times in the Changjiang River and its mouth during the last 40 years were analysed in this work. The inorganic N in precipitation in the Changjiang River catchment mainly comes from gaseous loss of fertilizer N, N resulting from the increases of population and livestock, and from high temperature combustions of fossil fuels. N from precipitation is the first N source in the Changjiang River water and the only direct cause of high content of inorganic N in the Changjiang River and its mouth. The lost N in gaseous form and from agriculture non-point sources fertilizer comprised about 60% of annual consumption of fertilizer N in the Changjiang River catchment and were key factors controlling the high content of inorganic N in the Changjiang River mouth. The fate of the N in precipitation and other N sources in the Changjiang River catchment are also discussed in this paper.