Encystment and excystment of <Emphasis Type="Italic">Gyrodinium instriatum</Emphasis> Freudenthal et Lee

In the present study, we have investigated the conditions influencing encystment and excystment in the dinoflagellate Gyrodinium instriatum under laboratory conditions. We incubated G. instriatum in modified whole SWM-3 culture medium and in versions of modified SWM-3 from which NO₃ ⁻, PO₄ ³⁻, NO₃ ⁻ + PO₄ ³⁻, or Si had been omitted and observed encystment. Percentage encystment was high in the media without N and without P, while the percentage encystment in the medium lacking both N and P was highest. Moreover, to investigate N or P concentration which induced the encystment, Gyrodinium instriatum was also incubated in media with different concentrations of inorganic N and P; the concentrations of NO₂ ⁻ + NO₃ ⁻ and PO₄ ³⁻ were measured over time. The precursors of cysts appeared within 2 or 3 days of a decrease in NO₂ ⁻ + NO₃ ⁻ or PO₄ ³⁻ concentration to values lower than 1 μM or 0.2 μM, respectively. When cysts produced in the laboratory were incubated, we observed excystment after 8–37 days, without a mandatory period of darkness or low temperature. We incubated cysts collected from nature at different temperatures or in the dark or light and observed excystments. Natural cysts excysted at temperatures from 10 to 30°C, in both light and dark, but excystment was delayed at low temperatures. These studies indicate that G. instriatum encysts in low N or P concentration and excysts over a wide temperature range, regardless of light conditions, after short dormancy periods.     

Experimental Study of Sulfur Solubility in Silicate Melts Coexisting with Graphite as a Function of Silicate Melt Composition

Abstract. Sulfur (S) solubility is determined in silicate melts, in particular with low-FeO content coexisting with graphite. In a high FeO range (>10 mol %), the S solubility is strongly dependent on the FeO content in silicate melt, which is in good agreement with previous works. In an intermediate FeO range (1∼10 mol %), the S solubility in melts with a multi-component system is higher than that in melts with a simple system. The SKα shift of the electron microprobe shows the dissolve sulfur is all sulfide. A positive dependence is observed between CaO and S content in the melt undoped with FeO, but the dissolved sulfur is sulfate. The relationship between melt composition, S content, and S oxidation state is discussed.     

Performances of Landfill Liners under Dry and Wet Conditions

This paper addresses the study conducted on the performance of landfill liner interface parameters. Interface shear strength parameters for various combinations of 9 different lining materials were studied and presented in this paper. This comprehensive testing program covers the interfaces between: (1) soil and compacted clay liner (CCL), (2) geomembrane (HDPEs or PVC) and soil, (3) geosynthetic clay liner (GCL)/CCL and soil, (4) geomembrane and geotextile, (5) geotextile and soil, (6) geotextile and GCL/CCL, and (7) geomembrane and GCL/CCL. The experiments were conducted for both at dry or optimum moisture condition and at saturated or wet condition. The interface performance under both conditions were compared to access the material performances. Tabulated summaries of interface test data under dry or optimum moisture condition (OMO) and saturated or wet condition are presented in the paper.     

Collapse and fragmentation of rotating magnetized clouds – II. Binary formation and fragmentation of first cores

Subsequent to Paper I, the evolution and fragmentation of a rotating magnetized cloud are studied with use of three-dimensional magnetohydrodynamic nested grid simulations. After the isothermal runaway collapse, an adiabatic gas forms a protostellar first core at the centre of the cloud. When the isothermal gas is stable for fragmentation in a contracting disc, the adiabatic core often breaks into several fragments. Conditions for fragmentation and binary formation are studied. All the cores which show fragmentation are geometrically thin, as the diameter-to-thickness ratio is larger than 3. Two patterns of fragmentation are found. (1) When a thin disc is supported by centrifugal force, the disc fragments into a ring configuration (ring fragmentation). This is realized in a rapidly rotating adiabatic core as  Ω > 0.2τ⁻¹_ff  , where Ω and  τ_ff  represent the angular rotation speed and the free-fall time of the core, respectively. (2) On the other hand, the disc is deformed to an elongated bar in the isothermal stage for a strongly magnetized or rapidly rotating cloud. The bar breaks into 2–4 fragments (bar fragmentation). Even if a disc is thin, the disc dominated by the magnetic force or thermal pressure is stable and forms a single compact body. In either ring or bar fragmentation mode, the fragments contract and a pair of outflows is ejected from the vicinities of the compact cores. The orbital angular momentum is larger than the spin angular momentum in the ring fragmentation. On the other hand, fragments often quickly merge in the bar fragmentation, since the orbital angular momentum is smaller than the spin angular momentum in this case. Comparison with observations is also shown.     

Behavior of zircon in the upper-amphibolite to granulite facies schist/migmatite transition, Ryoke metamorphic belt, SW Japan: constraints from the melt inclusions in zircon

Behavior of zircon at the schist/migmatite transition is investigated. Syn-metamorphic overgrowth is rare in zircon in schists, whereas zircon in migmatites has rims with low Th/U that give 90.3 ± 2.2 Ma U–Pb concordia age. Between inherited core and the metamorphic rim, a thin, dark-CL annulus containing melt inclusion is commonly developed, suggesting that it formed contemporaneous with the rim in the presence of melt. In diatexites, the annulus is further truncated by the brighter-CL overgrowth, suggesting the resorption and regrowth of the zircon after near-peak metamorphism. Part of the zircon rim crystallized during the solidification of the melt in migmatites. Preservation of angular-shaped inherited core of 5–10 μm in zircon included in garnet suggests that zircon of this size did not experience resorption but developed overgrowths during near-peak metamorphism. The Ostwald ripening process consuming zircon less than 5–10 μm is required to form new overgrowths. Curved crystal size distribution pattern for fine-grained zircons in a diatexite sample may indicate the contribution of this process. Zircon less than 20 μm is confirmed to be an important sink of Zr in metatexites, and ca. 35-μm zircon without detrital core are common in diatexites, supporting new nucleation of zircon in migmatites. In the Ryoke metamorphic belt at the Aoyama area, monazite from migmatites records the prograde growth age of 96.5 ± 1.9 Ma. Using the difference of growth timing of monazite and zircon, the duration of metamorphism higher than the amphibolite facies grade is estimated to be ca. 6 Myr.     

Characteristics of earthquake-induced landslides in a heavy snowfall region—landslides triggered by the northern Nagano prefecture earthquake, March 12, 2011, Japan

The northern Nagano Prefecture earthquake, M_JMA?6.7 (Mw?6.2), which is inferred to have been triggered by the huge (Mw?9.0) March 11, 2011 Tohoku earthquake, occurred on March 12, 2011, in northern Nagano Prefecture, an area in Japan famous for heavy snowfall. A large number of landslides were triggered by the 12 March earthquake, and it caused building damage in the area of the epicenter. To clarify characteristics of the distribution and dynamic behavior of these landslides, we analyzed aerial photographs and conducted field surveys in and around the epicentral area. Large-scale landslides with long distance run-outs are a remarkable characteristic of the landslides induced by this earthquake. The long travel distance is considered to be related to the thick snowpack at the time the earthquake occurred. Moderate scale deep-seated landslides and shallow landslides were also observed in the study area. Based on an analysis of landslides with the active fault on which the earthquake is believed to have occurred, most of these landslides were distributed on the hanging wall of the active fault, within a distance of 12?km from the fault.     

Internal structure of the Nojima Fault zone from the Hirabayashi GSJ drill core

Abstract The internal structures of the Nojima Fault, south-west Japan, are examined from mesoscopic observations of continuous core samples from the Hirabayashi Geological Survey of Japan (GSJ) drilling. The drilling penetrated the central part of the Nojima Fault, which ruptured during the 1995 Kobe earthquake (Hyogo-ken Nanbu earthquake) ( M 7.2). It intersected a 0.3 m-thick layer of fault gouge, which is presumed to constitute the fault core (defined as a narrow zone of extremely concentrated deformation) of the Nojima Fault Zone. The rocks obtained from the Hirabayashi GSJ drilling were divided into five types based on the intensities of deformation and alteration: host rock, weakly deformed and altered granodiorite, fault breccia, cataclasite, and fault gouge. Weakly deformed and altered granodiorite is distributed widely in the fault zone. Fault breccia appears mostly just above the fault core. Cataclasite is distributed mainly in a narrow (≈1 m wide) zone in between the fault core and a smaller gouge zone encountered lower down from the drilling. Fault gouge in the fault core is divided into three types based on their color and textures. From their cross-cutting relationships and vein development, the lowest fault gouge in the fault core is judged to be newer than the other two. The fault zone characterized by the deformation and alteration is assumed to be deeper than 426.2 m and its net thickness is > 46.5 m. The fault rocks in the hanging wall (above the fault core) are deformed and altered more intensely than those in the footwall (below the fault core). Furthermore, the intensities of deformation and alteration increase progressively towards the fault core in the hanging wall, but not in the footwall. The difference in the fault rock distribution between the hanging wall and the footwall might be related to the offset of the Nojima Fault and/or the asymmetrical ground motion during earthquakes.     

In Situ Measurement of the Lobate Ctenophore Bolinopsis Mikado (Moser) Abundance by the Video Recording System

A video recording system (VRS) with dark-field illumination was designed for continuous observation of the lobate ctenophore Bolinopsis mikado on spatial distribution, abundance and size distribution. In situ operation proved that the VRS was capable to discriminate B. mikado larger than 15 mm in total length at the towing speed around 0.5 m s^–1. The VRS was useful to determine spatial distribution of B. mikado biomass, since it measured the abundance and size composition simultaneously.     

Three-year investigations into sperm whale-fall ecosystems in Japan

We report the first study of sperm whale‐fall ecosystems, based on mass sinking of whale carcasses at shelf depths in the northwest Pacific. We conducted three observations over a 2‐year period on replicate sperm‐whale carcasses implanted at depths of 219–254 m off the southern part of Japan from July 2003 to August 2005. The study was made possible by a mass stranding of sperm whales in January 2002, and the subsequent sinking of 12 carcasses in the waters off Cape Nomamisaki. Dense aggregations of unique chemosynthesis‐based fauna had formed around the whale carcasses after 18 months (July 2003). The mytilid mussel Adipicola pacifica was the most abundant macrofaunal species and covered most of the exposed bone surfaces. The general composition of the fauna was similar to that of deep‐water reducing habitats, but none of the species appearing in this study has been found at hydrothermal vents, cold seeps or deep‐water whale falls. A new species of lancelet, which was the first record of the subphylum Cephalochordata from reducing environments, a new species of Osedax; a rarely encountered benthic ctenophore, and a rare gastropod species were discovered at this sperm whale‐fall site. Benthic communities were similar across all the carcasses studied, although the body sizes of the whales were very different. The succession of epifaunal communities was relatively rapid and the sulphophilic stage was considerably shorter than that of other known whale falls.     

Modified Method for the Analysis of Nitrogen Isotopic Composition of Oceanic Nitrate at Low Concentration

A method for the determination of the δ¹⁵N of nitrate in seawater described by Cline and Kaplan (1975) has been modified for application to low-level nitrate samples. We have minimized the reagent blank problem by replacing the Devarda's alloy with an aluminum reagent, and have also established a procedure that yields quantitative (93 ± 2%) extraction of nitrogen even at low nitrate levels. Though the amounts and the δ¹⁵N of the blank N varied from one reagent set to another, with these modifications, an overall N blank was reduced to approximately 0.80 ± 0.33 μmole N having an estimated δ¹⁵N value of −1.8‰. After blank and yield corrections, the measured isotopic composition of nitrate differed by approximately 0.1‰ from the actual value while the precision was within ±0.2‰ at the 1.25 μM level. The modified procedure was applied to seawater samples collected from the equatorial Pacific in order to compare the N blanks in field samples with those derived from laboratory experiments. The results support the suitability of the modified approach for isotopic analysis of oceanic nitrate in shallow water. This revised version was published online in July 2006 with corrections to the Cover Date.