棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

烟台套子湾八角深水港的开发研究

通过调查和比较，认为在我国渤海和北黄海南岸的套子湾八角海区是一个条件优越的深水港址，在阐述开发八角深水港的重要意义同时，根据城市布局规划和岸线及水域资源，参照国际港口未来发展趋势，结合我国对中、西部地区经济发展战略，对八角深水港的开发和布局进行了研究，为烟台市发展成为“现代化、国际型港口城市”，提出了港区未来建设的模式。     

不同氮源对微小亚历山大藻生长和毒素产生的影响

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.     

The distribution and geological significance of carbazole compounds in Silurian paleo-pools of the Tarim Basin,Northwest China

The results presented in this paper indicated that the carbazole-type compounds have high thermal stability and also show stability in oxidation and bio-degradation. This kind of compounds still has high concentrations and a complete distribution in the analyzed dry asphalt samples, showing that they are particularly useful in the study of hydrocarbon migration of the paleo-pools. The difference in the contents of nitrogen compounds in the Silurian dry asphalts from the Awati, Tabei and Tazhong areas is attributed to the difference in the extent of oxidation and （or） bio-degradation for the areas; the Awati and Tabei areas underwent relatively strong oxidation and bio-degradation. During the first stage of hydrocarbon pool formation in the Silurian system in the Tazhong and Tabei areas of the Tarim Basin （at the end of the Silurian period） and at the second stage in the Awati area （in Permian）, the hydrocarbons experienced a long-distance migration.     

Jurassic Tectonics of North China: A Synthetic View

This paper gives a synthetic view on the Jurassic tectonics of North China, with an attempt to propose a framework for the stepwise tectonic evolution history. Jurassic sedimentation, deformation and magmatism in North China have been divided into three stages. The earliest Jurassic is marked by a period of magmatism quiescence （in 205-190 Ma） and regional uplift, which are considered to be the continuation of the “Indosinian movement” characterized by continent-continent collision between the North and South China blocks. The Early to Middle Jurassic （in 190-170 Ma） was predominated by weak lithospheric extension expressed by mantle-derived plutonism and volcanism along the Yanshan belt and alongside the Tan-Lu fault zone, normal faulting and graben formation along the Yinshan- Yanshan tectonic belt, depression and resuming of coal-bearing sedimentation in vast regions of the North China block （NCB）. The Middle to Late Jurassic stage started at 165y.5 Ma and ended up before 136 Ma; it was dominated by intensive intraplate deformation resulting from multi-directional compressions. Two major deformation events have been identified. One is marked by stratigraphic unconformity beneath the thick Upper Jurassic molasic series in the foreland zones of the western Ordos thrust-fold belt and along the Yinshan-Yanshan belt; it was predated 160 Ma. The other one is indicated by stratigraphic unconformity at the base of the Lower Cretaceous and predated 135 Ma. During this last stage, two latitudinal tectonic belts, the Yinshan-Yanshan belt in the north and the Qinling-Dabie belt in the south, and the western margin of the Ordos basin were all activated by thrusting; the NCB itself was deformed by the NE to NNE-trending structural system involving thrusting, associated folding and sinistral strike-slip faulting, which were spatially partitioned. Foliated S-type granitic plutons aged 160-150 Ma were massively emplaced in the Jiao-Liao massif east of the Tan-Lu fault zone and indicate important crustal thicken     

三维地震技术在煤层顶板稳定性研究中的应用

龙固井田为全隐蔽的华北型煤田,位于巨野煤田中部,其首采扩大区主采煤层为3号煤层。考虑3煤层顶板稳定性主要受其顶板的构造信息和岩性信息影响,因此首先依据三维地震勘探综合解释成果及波阻抗反演解释成果对二者进行定量化,然后对波阻抗数据进行归一化处理,使得波阻抗数据和量化后的构造数据具有相同的变化范围及等量贡献。在此距离范围内构造和岩性的权值各为0．5,依此生成综合因素煤层顶板稳定性隶属度。分析3煤层顶板以上10m、20m处的综合因素煤层顶板稳定性隶属度图可以发现,该区3煤层顶板稳定性比较好。且其稳定性主要是受构造因素控制,岩性因素相对影响较小。     

内蒙古桌子山煤田火区特征及灭火方法探讨

内蒙古自治区桌子山煤田赋存着较为稀缺的煤种一焦煤,煤田内存在的火区不仪烧掉了宝贵的焦性煤炭资源,给煤矿生产带来严重的事故隐患,对当地生态环境也造成极大的破坏。经查明区内有26处子火区,分布面积约760万m^2,通过煤田地质特征、煤质特征、地表指示特征、同位素氡气的浓度相对分布特征、物性特征分析了区内煤层发火原因,根据桌子山煤田火区特点,建议采用浅剥、深灌与覆盖相结合的灭火方法,并对火区进行动态监测。     

Flux determination over a smooth surface under strongly unstable conditions

Careful micrometeorological measurements on an empty parking lot allowed determination of the surface fluxes of sensible heatH and of momentum by applying profile equations derived from Monin-Obukhov similarity theory with two sets of the stability correction function for momentum _m and sensible heat _h . These fluxes were compared with reference values ofH independently determined by means of an eddy correlation technique. In general, better agreement was found betweenH values derived from profiles with the stability functions of Brutsaert (1992) and referenceH values, than when the Businger-Dyer functions were used to deriveH. The disagreement in the latter comparison was especially serious under strongly unstable conditions, with the value ofy=–z/L (wherez is the height andL is the Obukhov length) larger than 10. A closer look at the procedure for calculatingH from the profiles revealed that the large differences between theH values derived with these two different versions of the stability correction functions were caused by the small differences of the _h values, and not by the larger differences of the _m values. This result stems from the strong sensitivity of the resultingH values on the choice of _h .     

地震前水氡短临异常判别指标及预报三要素的方法探讨

本文分析了中国大陆(重点是南北地震带北段)水氧异常变化,深入研究了有震与无震异常的时空分布特征、异常总频次、起始异常时间差等,发现有震异常往往呈现“同步性”或“群体性”特征,而大部分“零散性”异常则属于非地震异常。根据上述研究,本文还提出了利用水氡异常预报地震的发震时间、地点和震级的初步方法。