Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Electric Discharges in the Protoplanetary Nebula as a Source of Impulse Magnetic Fields to Promote Dust Aggregation

Lightning discharge generated in the protoplanetary nebula is viewed as a temporally isolated surge in the flow of electrically charged particles, similar to that of terrestrial lightning. If the current is intense enough, a powerful circular impulse magnetic field is generated around the instantaneous virtual electric conductor. Such magnetic field is capable of magnetizing dust grains containing ferromagnetic components present in its vicinity to their saturation levels. As a result, dust grains attract one another, forming the aggregates. This magnetically driven attraction suggests an important process possibly operational at an early stage of the planetary accretion. Based on both a classical model for electric conductor, and the theory of Lienard–Wiechert electromagnetic potentials, our calculations show that the magnetic impulse due to a discharge channel of a few cm in diameter transferring a charge of about 10⁴ electrons reaches as high as 10 T. At these magnetic fields, the ferromagnetic dust grains, and possibly the already-formed larger aggregates as well, are easily magnetized to the saturation levels, producing compact clusters exhibiting permanent magnetic moments.     

Resonant Periodic Orbits of Trans-Neptunian Objects

We study two and three-dimensional resonant periodic orbits, usingthe model of the restricted three-body problem with the Sun andNeptune as primaries. The position and the stability character ofthe periodic orbits determine the structure of the phase space andthis will provide useful information on the stability and longterm evolution of trans-Neptunian objects. The circular planarmodel is used as the starting point. Families of periodic orbitsare computed at the exterior resonances 1/2, 2/3 and 3/4 withNeptune and these are used as a guide to select the energy levelsfor the computation of the Poincaré maps, so that all basicresonances are included in the study. Using the circular planarmodel as the basic model, we extend our study to more realisticmodels by considering an elliptic orbit of Neptune and introducingthe inclination of the orbit. Families of symmetric periodicorbits of the planar elliptic restricted three-body problem andthe three-dimensional problem are found. All these orbitsbifurcate from the families of periodic orbits of the planarcircular problem. The stability of all orbits is studied. Althoughthe resonant structure in the circular problem is similar for allresonances, the situation changes if the eccentricity of Neptuneor the inclination of the orbit is taken into account. All theseresults are combined to explain why in some resonances there aremany bodies and other resonances are empty.     

F stars, metallicity and the ages of red galaxies at z > 1

We explore whether the rest-frame near-ultraviolet spectral region, observable in high-redshift galaxies via optical spectroscopy, contains sufficient information to allow the degeneracy between age and metallicity to be lifted. We do this by first testing the ability of evolutionary synthesis models to reclaim the correct metallicity when fitted to the near-ultraviolet spectra of F stars of known (subsolar and supersolar) metallicity. F stars are of particular interest because the rest-frame near-ultraviolet spectra of the oldest known elliptical galaxies at   z > 1  appear to be dominated by F stars near to the main-sequence turn-off.
We find that, in the case of the F stars, where the Hubble Space Telescope ultraviolet spectra have a high signal-to-noise ratio, fitting models in which the metallicity is allowed to vary as a free parameter is rather successful at deriving the correct metallicity. As a result, the estimated turn-off ages of these stars yielded by model-fitting are well constrained. Encouraged by this we have fitted these same variable-metallicity models to the deep, optical spectra of the   z ≃ 1.5 mJy  radio galaxies 53W091 and 53W069 obtained with the Keck telescope. While the age and metallicity are not so easily constrained for these galaxies, we find that even when metallicity is allowed as a free parameter, the best estimates of their ages are still ≥3 Gyr, with ages younger than 2 Gyr now strongly excluded. Furthermore, we find that a search of the entire parameter space of metallicity and star formation history using MOPED leads to the same conclusion. Our results therefore continue to argue strongly against an Einstein–de Sitter universe, and favour a Λ-dominated universe in which star formation in at least these particular elliptical galaxies was completed somewhere in the redshift range   z = 3–5  .     

An Instantaneous-Frequency Filtering Technique for High-Frequency Radar Oceanography

High-frequency (HF) radar systems are remote sensing tools that can be used to measure oceanographic parameters. Problems can occur when using the conventional periodogram (PG) method for computing power spectral estimates from backscattered radar signals. Temporal and spatial inhomogeneities within the radar measurement region can cause distortion in the spectra. This paper describes an instantaneous-frequency (IF) filtering technique that has been developed to measure the first-order modulation contained within the radar signal. Successful removal of this modulation is shown to yield an increased quality and quantity of ocean measurements     

Survival of organic phases in porous IDPs during atmospheric entry: A pulse‐heating study

Abstract— In this study, we have performed pulse‐heating experiments at different temperatures for three organic molecules (a polycyclic aromatic hydrocarbon [PAH], a ketone, and an amino acid) absorbed into microporous aluminum oxide (Al₂O₃) in order to imitate the heating of the organic molecules in interplanetary dust particles (IDPs) and micrometeorites (MMs) during atmospheric entry and to investigate their survival. We have shown that modest amounts (a few percent) of these organic molecules survive pulse‐heating at temperatures in the 700 to 900 °C range. This suggests that the porosity in IDPs and MMs, combined with a sublimable phase (organic material, water), produces an ablative cooling effect, which permits the survival of organic molecules that would otherwise be lost either by thermal degradation or evaporation during atmospheric entry.     

Investigation of Failure Mechanisms and Migration of Organic Chemicals at Wilsonville, Illinois

Ground water contamination was discovered in 1981 in a monitoring well at the Earthline disposal facility near Wilsonville, Illinois. Organic chemicals had migrated at a rate 100 to 1000 times greater than predicted when the site received its permit to operate in 1978. Postulated failure mechanisms included migration through previously unmapped permeable zones, subsidence of an underground mine, organic-chemical and clay-mineral interactions, acid-mine drainage and clay interactions, trench-cover settlement, and erosion.
In this investigation, the Illinois State Geological Survey found the primary reason for the rapid migration: the presence of previously undetermined fractures and joints in glacial till. The inaccurate predictions of hydraulic conductivity were based on laboratory-determined values that did not adequately measure the effects of fractures and joints on the transit time calculations. Field-measured hydraulic conductivity values were generally 10 to 1000 times greater than their laboratory-measured counterparts, thus largely accounting for the discrepancy between predicted and actual migration rates in the transit time calculations. The problem was compounded, however, by the burial of liquid wastes and by trench covers that allowed excess surface runoff to enter the trenches. Organic-chemical and clay-mineral interactions may also have exacerbated the problem in areas where liquid organic wastes were buried.