最小弧菌热不稳定型溶血素基因克隆与序列分析

根据已发表的最小弧菌热不稳定型溶血素（heat-labile hemolysin）基因核苷酸序列，设计和合成一对特异性引物，以最小弧菌96-6-3的基因组DNA为模板，PCR扩增得到热不稳定型溶血素基因片段，克隆到质粒载体pMD-18T中进行测序和分析．其结果表明扩增的热不稳定型溶血素基因片段与已发表的最小弧菌热不稳定型溶血素的同源性高达98％，扩增片段编码由446个氨基酸组成的多肽，推测分子量为50200．软件分析表明编码的氨基酸有较高的抗原性，可作为基因工程疫苗的候选基因片段．     

褐藻酸降解菌侵染海带过程的超微结构观察

首次研究了褐藻酸降解菌侵入海带的途径，通过电镜观察，跟踪研究了褐藻酸降解菌染海带过程中海带表皮细胞壁的超微结构变化。结果表明，褐藻酸降解侵染海带的过程是首先引起海带表皮细胞壁藻胶层表面的破坏，然后引起藻胶层的断裂，并使藻胶层逐渐降解变突，最后褐藻酸降解菌通过海带细胞的壁藻胶层的断裂变突处进入海带细胞内。     

论宁波东南港湾地区水资源的开发利用途径

本文在１９８９年对宁波市水资源进行调查的基础上，进一步分析了东南港湾地区水资源的特点及利用现状，并由此而提出了解决水源的对策。     

喀斯特荒漠化是一种地质—生态灾难

ＫＤＮ是一种ＧＥＯＬ－ＥＣＯＬ灾难,是发生潮湿的碳酸盐岩区的土壤侵蚀、土地退化与生态系统凋萎,致使人类很难生活在其中。ＫＤＮ是我国四大ＧＥＯＬ－ＥＣＯＬ灾害中最难整治、最难摆脱贫困的地区。本文按国际公认的原则为准,认定“石山化”≠ＫＤＮ,从地质结构性气候、物候和生态系统多因子厘定了ＫＤＮ术语,认定人为扰动与人口超载是其产生的序参量。其过程最迟也是３００多年前明清朝代屯垦开始,近半个世纪加速衰竭,森     

长江口羽状锋海区浮游植物的生态研究：—昼夜分布动态

本文对长江口羽状锋海区浮游植物的昼夜变动进行了初步研究，结果表明，浮游植物数量的昼夜变化明显，潮汐作用是影响数量昼夜分布变化的主导因素，同时也与该地区浮游植物的水平分布有着密切的联系。     

南四湖,东平湖资源综合开发问题探讨

南四湖，东平湖是华北重要的淡水湖，湖区自然条件优越，资源丰富，有巨大开发潜力。本文分析湖区资源特征，开发利用现状和存在问题，提出合理开发利用途径与对策。     

灵山岛浅海岩礁区底栖生物的群落特点

浅海岩礁环境系高生产力区,在中、高纬度海域中,由大型海藻和大叶藻(Zosteramarina)所构成的初级生产力常为浮游植物初级生产力的数倍(Miller et al.,1971)。同时,此种环境又是以大型藻类或有机碎屑为食物的珍贵海产品鲍鱼(Haliotis)、刺参(Stichopus)、海胆(Strongylocentrotus)等的栖息场所和采捕作业区;还是一些优质鱼类星鳗( Astroconger myriaster)、鲈(Lateolobrax japonicus)等的藏匿场所和游钓渔业的渔场;重要经济藻类石花菜(Gelidium)等也在此环境中定着、生长。所以,浅海岩礁的资源开发前景很好。为了促进上述珍贵海产品的增、养殖生产和发展游钓渔业,有必要对浅海岩礁底栖生物群落的结构、功能进行系统研究,以便为岩礁区的资源开发和海岸带环境保护提供科学依据。为此,中科院海洋研究所在1987年初步调查的基础上,1992年以灵山岛为点,对水下岩礁环境和生物群落进行了系统的调查。本文主要分析浅海岩礁底栖生物的群落特点。     

Identification of Pb sources in Yellow Sea sediments using stable Pb isotope ratios

Rapid economic developments in East Asian countries have inevitably resulted in environmental degradation in the surrounding seas, and concern for both the environment and protection from pollutants is increasing. Identification of sources of contaminants is essential to environmental pollution management. In this study, the provenance of anthropogenic lead (Pb), a major pollutant of Yellow Sea sediments, was determined for river mouth sediments, including those of the Changjiang, Huanghe, Han, and Geum Rivers, and for age-determined shelf core sediments through the measurement of Pb isotope ratios in the HCl-leached fraction using multi-collector inductively coupled plasma-mass spectrometry (MC ICP/MS). Anthropogenic Pb has accumulated in shelf core sediments since 1910, and its isotope ratios were estimated as 0.863–0.866 and 2.119–2.125 for ²⁰⁷Pb/²⁰⁶Pb and ²⁰⁸Pb/²⁰⁶Pb, respectively, from the mixing relationships of the two endmembers. River mouth sediments exhibited enough distinction in anthropogenic Pb isotope ratios to be discriminated: 0.874 (2.144) in the Huanghe, 0.856 (2.129) in the Han, 0.857 (2.122) in the Geum, and 0.854 (2.101) in the Changjiang for ²⁰⁷Pb/²⁰⁶Pb (²⁰⁸Pb/²⁰⁶Pb), respectively. Although isotope ratios of geogenic Pb in sediments dating before 1910 showed narrow ranges (0.842–0.845 and 2.088–2.100 for ²⁰⁷Pb/²⁰⁶Pb and ²⁰⁸Pb/²⁰⁶Pb, respectively), distinct isotope ratios in each core permitted source identification of sediments in the Yellow Sea based on geographic locations and the geogenic Pb of each river. By comparing the isotope ratios of the estimated anthropogenic Pb to source-related materials, the provenances of anthropogenic Pb in Chinese river sediments were presumed to be Chinese coal or ore, which is also a major source of atmospheric particulate Pb. The anthropogenic Pb in the shelf core sediments in the northern Yellow Sea originated from northern Chinese cities such as Beijing and Tianjin through atmospheric pathways. Pb isotope ratios indicated that Pb in Korean river sediments was characteristic of local Korean ores.     

三角褐指藻Phaeodactylum tricornutum的亚硝酸盐释放及其生态学意义

本文在藻类不同生长时期、光照、黑暗和光照+DCMU以及不同硝酸盐浓度、温度和pH条件下,研究了环境因子对三角褐指藻亚硝酸盐释放的影响。研究结果表明:三角褐指藻在光照、硝酸盐浓度大于10μmol/L、温度范围18～22℃和pH为8左右条件下,亚硝酸盐释放速率最大。亚硝酸盐在特定条件下的释放对于我们了解海洋中由浮游植物产生的亚硝酸盐第一最大层的形成机制有着十分重要的意义。     

Determination of palladium and platinum in seaweed

Palladium and platinum concentrations were determined for twenty two species of seaweed from the California coast, U.S.A.. A new sensitive analytical technique using atomic absorption spectrometry was developed for palladium in seaweed. For the first time, palladium contents in seaweed were obtained. The contents of palladium and platinum varied from 0.09 to 0.61 ng g^–1 and 0.25 to 1.75 ng g^–1 in dried material, respectively.The average ratio of Pt/Pd found in seaweed was 3.5, while the ratio of Pt/Pd was 4.5 in seawater. Therefore, it is postulated that there is no discrimination for seaweed to accumulate palladium and platinum from the ambient seawater due to the chemical similarity of the metals.