对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

Laboratory tests of vortex-induced vibrations of a long flexible riser pipe subjected to uniform flow

Laboratory tests have been conducted on vortex-induced vibration (VIV) of a long flexible riser towed horizontally in a wave basin. The riser model has an external diameter of 16 mm and a total length of 28.0 m giving an aspect ratio of about 1750. Reynolds numbers ranged from about 3000 to 10,000. Fiber optic grating strain gages are adopted to measure the dynamic response in both cross-flow and in-line directions. The cross-flow vibrations were observed to vibrate at modes up to 6 and the in-line reached up to 12. The fundamental response frequencies can be predicted by a Strouhal number of about 0.18. Multi-mode responses and the asymmetry of the bare pipe response in uniform flow were observed and analyzed. The experimental results confirmed that the riser pipe vibrated multi-modally despite it being subject to a uniform current profile and all of the excited modes vibrated at the Strouhal frequency. The asymmetrical distribution of displacement mainly resulted from the modal composition. Higher harmonics of the VIV response such as the third, fourth and fifth harmonics frequencies were found to be steady over the entire duration of the test even if they varied along the length of the riser pipe.     

Hydrodynamic characteristics and sediment transport of a tidal river under influence of wading engineering groups

By taking the Yong River for example in this paper, based on the multiple measured data during 1957 to 2009, the change process of runoff, tide feature, tidal wave, tidal influx and sediment transport are analyzed. Then a mathematical model is used to reveal the influence mechanism on hydrodynamic characteristics and sediment transport of the wading engineering groups such as a tide gate, a breakwater, reservoirs, bridges and wharves, which were built in different periods. The results showed the hydrodynamic characteristics and sediment transport of the Yong River changed obviously due to the wading engineering groups. The tide gate induced deformation of the tidal wave, obvious reduction of the tidal influx and weakness of the tidal dynamic, decrease of the sediment yield of flood and ebb tide and channel deposition. The breakwater blocked estuarine entrances, resulting in the change of the tidal current and the reduction of the tidal influx in the estuarine area. The large-scale reservoirs gradually made the decrease of the Yong River runoff. The bridge and wharf groups took up cross-section areas, the cumulative affection of which caused the increase of tidal level in the tidal river.     

内核及外围尺度对热带气旋强度影响的数值试验

采用WRF数值模式模拟并对热带气旋尺度与强度关系进行了探讨,且初步诊断分析了内核及外围尺度对热带气旋强度影响的可能机制,结果表明:(1)内核区较大时的缩放引起角速度变化是其影响热带气旋强度的机制之一;(2)内核区较小时的进一步收缩引起的眼区次级环流破坏是其影响热带气旋强度的一种机制;(3)外围尺度变化造成低层上升至高层的水汽总量变化,是其影响热带气旋强度的一种机制;另一方面,外围尺度发生变化使得低层气流向热带气旋内的辐合减少,亦是其影响热带气旋强度的机制之一。     

天然叶蜡石及其热处理产物的电子顺磁共振（EPR）研究

利用EPR研究了18个天然叶蜡石样及其4个热处理产物。发现天然叶蜡石有三个EPR吸收带：A（g有效≈11．4），B（g有效≈4．3）和C（g有效≈2．0），其中A是叶蜡石的特征谱。A在＞400℃热处理时与B在强度上呈消长关系；＜400℃热处理时A和B均随温度升高而加强；900℃时A消失，B的强度远远大于原样中的A；C附有超精细结构谱（g″=2.04,g┴=1.96,A″≈A┴=19.5×10^-4T），C在热处理时呈现复杂的变化。结果表明，A吸收带属于叶蜡石八面体AI（Fe)-O4(OH)2中的Fe^3 ，B吸收带归于八面体AI（Fe)-O2(OH)4中的Fe^3 ，C吸收带是无机自由基（如AI－O^-Si)和有机自由基的叠加谱；900℃热处理后八面体层中配位发生了变化：AI（Fe)-O4(OH2)→AI（Fe)-O5 H2O↑，AI(Fe)-O2(OH)4→AI(Fe)-O4 H2O↑；类质同象置换AI^3 的不仅有Fe^3 ,而且有Fe^2 离子。     

南海周边相对海平面变化特征及2004年苏门答腊地震影响分析

利用南海周边1989-2014年的潮汐资料和GPS长期观测资料,分析了南海周边相对海平面变化特征,以及2004年苏门答腊地震对该区域相对海平面变化的影响。研究结果表明,南海周边的相对海平面变化以上升为主,平均上升速率（4.53±0.20） mm/a,高于全球平均速率,且2004年后上升趋势加剧;南海周边相对海平面呈现6类较典型的变化特征,并存在与板块构造相对应的分区聚集现象,形成了中国东南和越南沿海、马来半岛、加里曼丹岛北部、菲律宾群岛等4个变化特征区。受2004年苏门答腊大地震的影响,马来半岛、南沙和西沙海域的地壳形变由上升趋势转为下沉,加剧了相对海平面的上升;中国东南沿海和菲律宾群岛受地震影响较小;越南沿海和加里曼丹岛北部区域的地震影响还有待进一步研究。     

Tropical cyclones and multiscale climate variability: The active western North Pacific Typhoon season of 2018

The 2018 typhoon season in the western North Pacific(WNP) was highly active, with 26 named tropical cyclones(TCs) from June to November, which exceeded the climatological mean(22) and was the second busiest season over the past twenty years. More TCs formed in the eastern region of the WNP and the northern region of the South China Sea(SCS). More TCs took the northeast quadrant in the WNP, recurving from northwestward to northward and causing heavy damages in China's Mainland(69.73 billion yuan) in 2018. Multiscale climate variability is conducive to an active season via an enhanced monsoon trough and a weakened subtropical high in the WNP. The large-scale backgrounds in 2018 showed a favorable environment for TCs established by a developing central Pacific(CP) El Ni?o and positive Pacific meridional mode(PMM)episode on interannual timescales. The tropical central Pacific(TCP) SST forcing exhibits primary control on TCs in the WNP and large-scale circulations, which are insensitive to the PMM. During CP El Ni?o years, anomalous convection associated with the TCP warming leads to significantly increased anomalous cyclonic circulation in the WNP because of a Gill-type Rossby wave response. As a result, the weakened subtropical high and enhanced monsoon trough shift eastward and northward, which favor TC genesis and development. Although such increased TC activity in 2018 might be slightly suppressed by interdecadal climate variability, it was mostly attributed to the favorable interannual background. In addition, high-frequency climate signals,such as intraseasonal oscillations(ISOs) and synoptic-scale disturbances(SSDs), interacted with the enhanced monsoon trough and strongly modulated regional TC genesis and development in 2018.     

2.5-D DC Resistivity Modeling Considering Flexibility and Accuracy

We highlighted the flexibility of using unstructured mesh together with the local refinement by a resistivity model with complicated topography. The effect of topography is emphasized. Based on this, we calculated a specific class of layered models and found that the accuracy is not always satisfactory by utilizing the standard approach. As an improvement, we employed the layered earth as the reference model to calculate the wavenumbers. The comparison demonstrates that the accuracy is considerably improved...     

PROTEM-37瞬变电磁测深系统及应用实例

PROTEM - 37仪器具有分辨率高、动态范围大、抗干扰能力强、探测深度较大等优良性能。简要介绍了该仪器的测深原理、工作装置、野外施工步骤以及资料的处理解释。对TEM和MT资料的联合反演作了有益的尝试。介绍了该仪器在探测地下洞穴、勘察金属矿以及煤田水文地质调查等方面