Monitoring of DSP toxins in small-sized plankton fraction of seawater collected in Mutsu Bay,Japan, by ELISA method: relation with toxin contamination of scallop

Monitorings were conducted on DSP toxins in mid-gut gland of scallop (mouse assay), cell numbers of toxic dinoflagellate species of Dinophysis, and diarrhetic shellfish poisoning (DSP) toxins in small-sized (0.7-5 microm) plankton fraction of seawater collected from surface (0 m) and 20 m depth at a station in Mutsu Bay, Aomori Prefecture, Japan, in 2000. A specific enzyme-linked immunosorbent assay (ELISA) was employed for the analysis of DSP toxins in small-sized plankton fraction using a mouse monoclonal anti-okadaic acid antibody which recognizes okadaic acid, dinophysistoxin-1, and dinophysistoxin-3. DSP toxins were detected twice in the mid-gut gland of scallops at 1.1-2.3 MU (mouse units) g(-1) on 26 June and at 0.6-1.2 MU g(-1) on 3 July, respectively. Relatively high cell densities of D. fortii were observed on 26 June and 11 September, and may only contribute to the bivalve toxicity during late June to early July. D. acuminata did not appear to be responsible for the toxicity of scallops in Mutsu Bay in 2000. ELISA monitoring of small-sized plankton fraction in seawater could detect DSP toxins two weeks before the detection of the toxin in scallops, and could do so two weeks after the loss of the bivalve toxicity by mouse assay. On 17 July, toxic D. fortii was detected at only small number, <10 cells l(-1), but DSP toxins were detected by the ELISA assay, suggesting a presence of other toxic small-sized plankton in seawater. For the purpose of reducing negative impacts of DSP occurrences, monitorings have been carried out hitherto on DSP toxins of bivalve tissues by mouse assay and on cell densities of "toxic" species of Dinophysis. Here we propose a usefulness of ELISA monitoring of plankton toxicity, especially in small-sized fraction, which are possible foods of mixotrophic Dinophysis, as a practical tool for detecting and predicting DSPs in coastal areas of fisheries grounds of bivalve aquaculture.     

The Uptake Coefficient of I2 on Various Aqueous Surfaces

Uptake coefficient of I₂ on aqueous surfaces was measured by using an impinging flow method. Dependence of the uptake coefficient on the gas-liquid contact time and pH was investigated. The uptake coefficient was (3.7± 2.0)×10^–4 at 120 ms, 293 K and pH = 5.6. In the alkaline region, the uptake coefficient was larger by one order of magnitude than that in the neutral and acidic region. The I₂ uptake on KI solutions and synthetic sea water solutions was also measured. According to the analysis by the reactive uptake model, the uptake of I₂ was shown to be mainly determined by liquid phase reactions and the accommodation coefficient of I₂ on aqueous surfaces was estimated to be 0.01.     

Pyroxenes from the Beaver Bay gabbro complex of Minnesota

Although the Beaver Bay ferrogabbro is a small-scale layered intrusion, Ca-rich pyroxenes show a strong iron enrichment during fractionation, ranging from augite (Mg₃₈Fe₂₄ Ca₃₈) to ferrohedengergite (Mg₁₀Fe₄₈Ca₄₂). Ca-poor pyroxenes from intermediate pigeonite (Mg₃₉Fe₅₀Ca₁₁) to ferriferous pigeonite (Mg₂₇Fe₆₅Ca₈) occur as intercumulus minerals. The pyroxenes from the non-layered Beaver River gabbro are included in the overall pyroxene fractionation trend of the Beaver Bay gabbro complex. The pyroxene trend of the Beaver Bay gabbro complex is similar to those of the Skaergaard and Bushveld; however, there is a slight difference in that the Ca-rich pyroxenes of Beaver Bay (having Mg content over 30%) are slightly richer in Ca than either the Skaergaard or Bushveld augites.     

Stratospheric aerosol inferred from electrical conductivity during a volcanically quiescent period

Profiles of electrical conductivity in the troposphere and stratosphere were measured by balloon-borne conductivity sondes at Garmisch-Partenkirchen, West Germany, from January to May, 1980, when volcanic activity was low. The aerosol concentration has been deduced from the relative decrease of conductivity from surrounding values by assuming the effective attachment coefficient of ions to aerosols. A prominent decrease of the conductivity near the tropopause is usually observed indicating high concentrations of Aitken particles (500–1000 cm^–3). A decrease of conductivity, well above the tropopause, is sometimes observed, probably due to the transport of tropospheric Aitken particles with high concentration (200–400 cm^–3) into the stratosphere.     

Numerical studies on the two-dimensional flow in horizontally homogeneous canopy layers

The two-dimensional equation of motion containing the pressure gradient and Coriolis force is numerically solved for the wind field in and above the layers of a horizontally homogeneous canopy with a vertical distribution of leaf-area densities. The solution shows that, in the case of descending through the canopy, the wind vector turns with an angle which depends on the profile of leaf-area densities. In particular, for the canopy of a forest consisting of upper layers with higher densities and lower layers with smaller densities, the turning is striking; a secondary maximum in wind profile appears in the lower layers.Variations of the aerodynamic parameters for the flow above the canopy are indicated with respect to the leaf-area density. The roughness length varies in such a manner that a maximum appears in intermediate density values, depending on the shape of the profile of leaf-area density. In the case of very dense canopies, the shearing stress acting on the flow above the canopy is determined by the contribution from only the upper canopy elements, but not by that from the lower parts of the canopy.     

Air-sea bulk transfer coefficients in diabatic conditions

On the basis of recent data for the roughness Reynolds number of the sea surface, and using the Owen-Thomson theory on the transfers of heat and mass between a rough surface and the flow above it, the bulk transfer coefficients of the sea surface have been estimated. For a reference height of 10 m, the neutral-lapse transfer coefficient for water vapor is larger by only a few percent than that for sensible heat. When the wind speed at the 10-m height is u ₁₀>3 m s^–1, the coefficient for sensible heat C _H is larger by about 10% than that for momentum C _D . For u ₁₀<5 m s^–1, however, the value of C _D exceeds the value of C _H , and for u ₁₀=15 m s^–1 it is shown that C _H 0.8C _D . It may be also proposed that 10³ C _D =1.11 to 1.70, 10³ C _E =1.18 to 1.30, and 10³ C _H =1.15 to 1.26 for a range of u ₁₀=4 to 20 m s^–1. A plot of diabatic transfer coefficients versus wind speed is obtained by using a parameter of the sea-air temperature difference. For practical purposes, the coefficients are approximated by empirical formulae.     

Deccan basalts at Mahabaleshwar,India

Fortyone successive flows of the Deccan Traps have been investigated at Mahabaleshwar, India, and the rocks from the twenty two different flows have been newly analysed. All of these basalts are silica-saturated tholeiites; and the series shows minor gradual variation with the order of eruption. These seem to be a result of magmatic differentiation somewhat similar to that shown in the Skaergaard intrusion.     

Gamma-ray bursts observed from the Hinotori satellite

During February, 1981 and June, 1982 the gamma-ray and the hard X-ray spectrometers on the Hinotori satellite observed four gamma-ray bursts on 28 February, 21 July, 1981, 26 February and 13 March, 1982. These gamma-ray bursts were simultaneously observed by other satellites. The time histories and energy spectra are shown for these gamma-ray bursts, and the burst sizes (erg cm^–2) are estimated. Two possible source locations for the burst of 21 July, 1981 are roughly determined from arrival time delays between two pairs of satellites, PVO-Hinotori and ISEE-3-Hinotori. The weak gamma-ray line peak structure around 1.8 MeV was observed for the burst of 13 March, 1982. The line could be interpreted in terms of gravitationally redshifted neutron capture line at 2.22 MeV.     

On the physical parameters for Centaurus X-3 and Hercules X-1

Upper and lower limits on the physical parameters for Cen X-3 and Her X-1 have been computed from a simple assumption involving the mass function.     

Radiation Pressure and the Poynting-Robertson Effect for Fluffy Dust Particles

A correct understanding of the dynamical effect of solar radiation exerted on fluffy dust particles can be achieved with assistance of a light scattering theory as well as the equation of motion. We reformulate the equation of motion so that the radiation pressure and the Poynting-Robertson effect on fluffy grains are given in both radial and nonradial directions from the center of the Sun. This allows numerical estimates of these radiation forces on fluffy dust aggregates in the framework of the discrete dipole approximation, in which the first term of the scattering coefficients in Mie theory determines the polarizability of homogeneous spheres forming the aggregates.The nonsphericity in shape turns out to play a key role in the dynamical evolution of dust particles, while its consequence depends on the rotation rate and axis of the grains. Unless a fluffy dust particle rapidly revolves on its randomly oriented axis, the nonradial radiation forces may prevent, apart from the orbital eccentricity and semimajor axis, the orbital inclination of the particle from being preserved in orbit around the Sun. However, a change in the inclination is most probably controlled by the Lorentz force as a consequence of the interaction between electric charges on the grains and the solar magnetic field. Although rapidly and randomly rotating grains spiral into the Sun under the Poynting-Robertson effect in spite of their shapes and structures, fluffy grains drift inward on time scales longer at submicrometer sizes and shorter at much larger sizes than spherical grains of the same sizes. Numerical calculations reveal that the dynamical lifetimes of fluffy particles are determined by the material composition of the grains rather than by their morphological structures and sizes. The Poynting-Robertson effect alone is nevertheless insufficient for giving a satisfactory estimate of lifetimes for fluffy dust grains since their large ratios of cross section to mass would reduce the lifetimes by enhancing the collisional probabilities. We also show that the radiation pressure on a dust particle varies with the orbital velocity of the particle but that this effect is negligibly small for dust grains in the Solar System.