冬至初春黄海暖流的路径和起源

主要根据近几年来中韩黄海水循环动力学合作调查结果,结合有关观测资料,进一步分析了冬至初春黄海暖流的路径和起源.与以往类似研究不同的主要有两点:(1)初步探讨了黄海暖流路径的季节和年际变异,并指出这种变异与北向风的强弱密切相关;(2)通过分析济州岛西侧海域混合水的去向,进一步确认了部分混合水绕济州岛运行,并进入济州海峡这一事实.同时,初步揭示进入黄海的混合水,即黄海暖流水,含有更多的东海陆架水成分.     

乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达

采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。     

Dissipation source function and an improvement to LAGFD-WAM model

In this paper, a new theoretical expression of dissipation term is presented on the basis of statistical model of breaking wave, which is an improvement to LAGFD-WAM wave model. The computational results in three typical wind fields show a good improvement to LAGFD-WAM model and a better accuracy in comparison with the observed data in the South China Sea.     

A study on remote sensing models of sea ice thickness by microwave radiometry

Extrapolating from the propagation theories of electromagnetic waves in a layered medium, a three-layer medium model is deduced in this paper by using microwave radiometric remote sensing technology which is suitable to first-year sea ice condition of the northern part of China seas. Comparison with in situ data indicates that for microwave wavelength of 10 cm, the coherent model gives a quite good fit result for the thickness of sea ice less than 20 cm, and the incoherent model also works well for thickness within 20 to 40 cm. Based on three theoretical models, the inversion soft ware from microwave remote sensing data for calculating the thickness of sea ice can be set up. The relative complex dielectrical constants of different types of sea ice in the Liaodong Gulf calculated by using these theoretical models and measurement data are given in this paper. The extent of their values is (0. 5-4. 0)-j(0. 07~0. 19).     

Robust and adaptive path following for underactuated autonomous underwater vehicles

This paper proposes a nonlinear robust adaptive control strategy to force a six degrees of freedom underactuated underwater vehicle with only four actuators to follow a predefined path at a desired speed despite of the presence of environmental disturbances and vehicle’s unknown physical parameters. The proposed controller is designed using Lyapunov’s direct method, the popular backstepping and parameter projection techniques. The closed loop path following errors can be made arbitrarily small. Interestingly, it is shown that our developed control strategy is easily extendible to situations of practical importance such as parking and point-to-point navigation. Numerical simulations are provided to illustrate the effectiveness of the proposed methodology.     

海水中液-固界面的台阶型动力学曲线 Ⅰ.动力学曲线之台阶的消长和变化规律

对海水中Zn(Ⅱ),Ca(Ⅱ),Cd(Ⅱ)与高岭石、伊利石和蒙脱石等粘土矿物;无定形水合氧化铁、α-FeOOH等铁的水合氧化物;δ-MaO_2,γ-MnOOH,水锰矿等锰的水合氧化物等30个左右实验体系的液-固界面台阶型动力学曲线进行了系统的实验测定和全面的条件研究。在实验测定上提出两种方法、互为校核。对动力学曲线之台阶的消长和变化规律作了系统研究,确定主要影响因素是:金属离子初始浓度、固体交换剂量、体系的pH值和温度,以及搅拌速度等。     

甲状腺素对斜带石斑鱼仔鱼变态的影响

用甲状腺素(Thyroxine,T4)处理斜带石斑鱼Epinepheluscoioides仔鱼,研究T4对仔鱼变态的影响。仔鱼在变态前期和变态高峰期,用0.01mg·L-1T4处理7d后仔鱼完成变态,用0.10mg·L-1T4处理4d后仔鱼完成变态,用1.00mg·L-1T4处理3d后仔鱼完成变态,对照组仔鱼在实验期间未能完成变态。用0.01mg·L-1T4处理变态高峰期仔鱼其存活率明显提高和全长增长明显变慢,但对变态前期仔鱼无影响;用0.10和1.00mg·L-1T4处理变态前期和变态高峰期仔鱼,其存活率显著降低和全长增长明显变慢。结果表明,在变态高峰期用0.01mg·L-1T4处理仔鱼能加速它的变态并提高其存活率。     

温度、光强和盐度对鹧鸽菜幼体生长发育的影响

从野外采集鹤鸪菜Ｃａｌｏｇｌｏｓｓａｌｅｐｒｉｅｕｒｉｉ（Ｍｏｎｔ．）Ｊ．Ａｇ．幼体，在室内控制条件下，以温、盐、光等不同条件进行模拟培养试验。所获得的数据经生物统计检验表明：鹧鸪菜幼体生长最适水温为２３℃；光照强度为４０００ｌｘ；海水盐度为１８。此结果与我们多年来在汕头港内观察鹧鸪菜的生长及其季节变化相似。     

中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究

分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。     

分潮波模型多资料数值计算方法研究 Ⅰ.岸边资料运动学外推的第一边值方法

对于含多资料三维分潮波模型建立了一种岸边资料运动学外推的潮波第一边值方法，并对渤海的四大原湖波进行试算。结果证实本方法可行，该方法使潮波方程既满足第一边值问题，充分利用岸边测站资料，又保证岸界法向流速为零条件，符合物理意义。并对复型病态矩阵的收敛问题提出了一种可行性处理。