Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

斜坡提砌石护面厚度的计算

介绍了斜坡式防波堤砌石护面中的插砌条石和干砌块石在波浪作用下的力学图式及作用机理 ,重点给出考虑摩擦力作用时的砌石块体稳定厚度的计算公式 ,并结合工程实例验证了公式的适用性     

似海底反射的地震速度和AVO效应

我们将浓度和压力作为一个函数得到冰和含气体水合物的地震波速度。同以往以简单的慢度和平均模量及双相模型为基础的理论不同，我们使用了Biot类型的三相理论，这一理论考虑了二种固体（颗粒和冰或笼形物）和一种液体（水）的存在，以及一个含气和水的多孔隙基质。对于固体的Berea砂岩，低于0℃时的纵波理论估计值过低，在含有冰－颗粒相互作用和颗粒胶结时与实验数据产生了好的吻合。严格地讲，水的比例与温度紧密相关。假定已知孔隙的平均半径和标准偏差，那么在一给定温度下的波速拟合就能对整个温度范围的速度进行预测。通过一个粘弹性的单相结构模型来计算反射系数。本文的分析是针对游离气带的顶部（位于含气体水合物的沉积层下）和底部（上履于饱含水的沉积层）进行的。如果似海底反射仅仅是由于胶结气体水合物与含游离气沉积层的分界面造成，我们可以得出结论：（1）在一定的气体饱和度下，很难估算低浓度情况下气体水合物数量。然而，水合物的高、低浓度还是可以区分的，因为它们分别呈现正和负的异常；（2）游离气和饱和度可以从反射振幅中而不是从异常类型中获取；（3）P波转化为S波的反射系数是指示高含量的气体水合物和游离气一个较好指示。另一方面，对于未胶结的沉积物，振幅随偏移距变化曲线总是为正。     

两株琼胶酶高产细菌的筛选和鉴定

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。     

胶州湾夏季异养浮游细菌的时空变化规律及影响因素

2001年夏季对胶州湾异养浮游细菌在一个潮周期内的变化规律及影响因素进行了研究。结果表明,胶州湾异养浮游细菌数量的垂直分布特征是表层大于底层,表层平均8.99×109个/L,底层平均5.23×109个/L。胶州湾水体异养浮游细菌日变化幅度在表层水体较为明显,底层相对较小,但其变化规律均为最高值在小潮期而最低值出现在大潮期。浮游动物摄食、浮游植物光合作用产生的溶解有机物及水温和日光中的紫外辐射是影响胶州湾异养浮游细菌昼夜变化的主要因素,水交换是影响其日变化的主要因素。     

NOAA的海洋遥感

美国国家海洋与大气管理局(NOAA)下属的国家海洋服务处(NOS)对遥感应用有很长的历史，负责对海岸带和海洋环境进行实时监测。美国建议整合海洋观测系统，以便保持其在海洋监测和研究领域中的领先地位。本文简述了该机构利用遥感方法在海岸带及海洋监测和研究中的应用。     

胶州湾围隔浮游生态系统氮、磷营养盐迁移-转化模型研究

通过对目前生态动力学模型的总结和综合,以生态系统中氮、磷营养盐循环为主线,建立了适用于海洋围隔浮游生态系统的多变量的营养盐迁移-转化动力学模型.该模型包括浮游植物、浮游动物、溶解无机态营养盐、溶解有机态营养盐和生物碎屑5个模块,涉及溶解无机氮、磷酸盐、溶解有机氮、溶解有机磷、浮游植物、浮游动物和生物碎屑7个状态变量.分别利用1999年秋季和2000年夏季胶州湾围隔生态实验数据进行了模型和验证工作,成功地模拟了富加营养盐条件下围隔浮游生态系统中氮、磷营养盐生物化学迁移-转化过程,并确定了20余个参数的量值.     

Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.