海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Preliminary report on radionuclide concentrations in the bottom waters at the entrance of Wakasa Bay with special reference to the Japan Sea Proper Water

Marked fluctuation of concentrations of⁹⁰Sr and¹³⁷Cs was observed in the bottom waters at the entrance of Wakasa Bay during 1987–1992, and the cause was investigated. The concentrations of⁹⁰Sr and¹³⁷Cs in the bottom waters were significantly low when the upper level of the Japan Sea Proper Water (JSPW) was high and covered the sampling depth, but high when the upper level of the JSPW was low. The cause of the fluctuation observed in the bottom waters is, therefore, suggested to be the vertical fluctuation of the upper level of the JSPW on the shelf slope, which has been little described before.     

Controls on organic carbon distribution in sediments from the eastern Arabian Sea Margin

 Sediment cores from the upper continental slope of the eastern Arabian Sea have high organic carbon (OC), CaCO₃, and sand content at the top. The values decrease with increasing depth in the Holocene and Upper Pleistocene. Topographic highs show highest OC and lower CaCO₃ in the Holocene clayey sediments and vice versa in the Pleistocene sandy sediments. The OC is immature and marine or a mixture of both marine and terrestrial in the Holocene sediments and is mostly terrestrial and/or reworked marine in the Pleistocene sediments. Productivity is the main controlling factor for the organic carbon enrichment. Texture and reworking also influence the organic carbon variations. Received: 29 May 1996/Revision received: 10 January 1997     

A residual analysis method for quantitative comparison of spatial data from the Iceland-Faeroes Front

A spatial statistical method has been developed from the well-known Kriging technique in geostatistics, as a way of providing quantitative comparison between a pair of spatial data sets, and a measure for such a comparison. This residual analysis method is applied to oceanographic data in order to compare Iceland-Faeroes Front (IFF) model predictions against appropriate field observations, with an aim to assess the IFF model performance and its prediction accuracy. The method is also used to evaluate the model-generated dynamical variability within the model predictions, as well as the natural variability within the frontal observations. From the results, it has been found that the IFF model is highly robust, and gives better predictions at depth 150–350 m than elsewhere. Within such a depth (i.e. 250–350 m), there is also evidence to suggest that the main frontal region is most active above the IF Ridge. The natural variability obtained from the observations appears to be comparable to the model-generated dynamical variability after 20 days of integration, indicating a certain degree of accuracy in the model predictions. The method reported in this paper could also be extended for further use in model data assimilation. Thus, the work not only demonstrates how spatial statistics can be applied to oceanographic data, but also opens up new statistical tools for data handling in ocean modelling.     

Environmental overview of Eckernförde Bay,northern Germany

This environmental overview of Eckernförde Bay (northern Germany) summarizes the results of previous studies relevant to the Office of Naval Research's Coastal Benthic Boundary Layer (CBBL) Baltic field exercise conducted during 1993–1994. Significant environmental characteristics include the following: (1) surface sediment distribution is related to water depth, dictated primarily by hydrodynamic reworking of older glacial deposits; (2) the origin and characteristics of small-scale sedimentary structures depend on storm-generated waves and currents; (3) the proximity of the sea surface and sediment —water interface results in a pelagic—benthic coupling that drives biogeochemical processes and produces organicrich, acoustically turbid sediments; and (4) the bay floor is complicated topographically by pockmarks and manmade sedimentary structures.     

The synchronous coupling of a third-generation wave model and a two-dimensional storm surge model

The interaction of waves and currents is studied by the dynamical coupling of a third generation wave model and a two-dimensional storm surge model. The coupling process of the two models is implemented synchronously. To estimate the effects of waves on the generation of storm surges, the theory of Janssen is used. The effects of the wave radiation stress on surge levels and the effects of storm-induced currents on waves are also investigated.The coupled wave and storm surge models have been tested by hindcasting two storm events in the northern South China Sea. The use of the Simth and Banke stress relation underestimates the surges by 10%. The inclusion of the radiation stress improves the accuracy of the computed results slightly by 2%. The introduction of a wave-dependent surface drag gives a significant improvement. The storm-induced currents clearly affect the wave characteristics at the peak stage. However, as far as the prediction of wave height is concerned, it is better not to consider the wave radiation stress in the storm surge model unless this is accompanied by a wave-dependent surface drag.     

Voracious planktonic hydroids: unexpected predatory impact on a coastal marine ecosystem

Hydroids are typically attached, benthic cnidarians that feed on a variety of small prey. During sampling on Georges Bank in spring 1994, we found huge numbers of hydroids suspended in the plankton. They fed on young stages of copepods that are an important prey for fish, as well as on young fish themselves. Two independent methods were used to estimate feeding rates of the hydroids; both indicate that the hydroids are capable of consuming from 50% to over 100% of the daily production of young copepods. These results suggest that hydroids can have a profound effect on the population dynamics of zooplankton and young fish on Georges Bank.     

Three-dimensional visualization of mission planning and control forthe NPS autonomous underwater vehicle

The Naval Postgraduate School (NPS) is constructing a small autonomous underwater vehicle (AUV) with an onboard mission control computer. The mission controller software for this vehicle is a knowledge-based artificial intelligence (AI) system requiring thorough analysis and testing before the AUV is operational. The manner in which rapid prototyping of this software has been demonstrated by developing a controller code on a LISP machine and using an Ethernet link with a graphics workstation to simulate the controller's environment is discussed. The development of a testing simulator using a knowledge engineering environment (KEE) expert system shell that examines AUV controller subsystems and vehicle models before integrating them with the full AUV for its test environment missions is discussed. This AUV simulator utilizes an interactive mission planning control console and is fully autonomous once initial parameters are selected     

Bacterial sulphate reduction in sediments of a european salt marsh: Acid-volatile and tin-reducible products

The products of ³⁵S-sulphate reduction by sedimentary bacteria were measured at two sites in a salt marsh on the east coast of England. Non-acid-volatile products were measured, after acid-volatile sulphide was removed, by their reduction to sulphide by digestion with tin. The proportion of the sulphate reduced to tin-reducible products varied between 25% in a salt marsh pan and 61% in creek sediment, over a 0–25 cm depth profile. There were also variations with depth at each site in the proportions of sulphate reduced to tin-reducible products. Further examination revealed differences in the proportions of sulphate which were reduced to free sulphide, acid-volatile sulphide, sulphur or pyrite at the two sites. The data suggest that previous work which did not measure non-acid-volatile products underestimated sulphate reduction rates by three-fold in the creek site, but by only one third in the pan.