7. Ancient Fossil Record and Early Evolution (ca. 3.8 to 0.5 Ga)

Once life appeared, it evolved and diversified. From primitive living entities, an evolutionary path of unknown duration, likely paralleled by the extinction of unsuccessful attempts, led to a last common ancestor that was endowed with the basic properties of all cells. From it, cellular organisms derived in a relative order, chronology and manner that are not yet completely settled. Early life evolution was accompanied by metabolic diversification, i.e. by the development of carbon and energy metabolic pathways that differed from the first, not yet clearly identified, metabolic strategies used. When did the different evolutionary transitions take place? The answer is difficult, since hot controversies have been raised in recent years concerning the reliability of the oldest life traces, regardless of their morphological, isotopic or organic nature, and there are also many competing hypotheses for the evolution of the eukaryotic cell. As a result, there is a need to delimit hypotheses from solid facts and to apply a critical analysis of contrasting data. Hopefully, methodological improvement and the increase of data, including fossil signatures and genomic information, will help reconstructing a better picture of life evolution in early times as well as to, perhaps, date some of the major evolutionary transitions. There are already some certitudes. Modern eukaryotes evolved after bacteria, since their mitochondria derived from ancient bacterial endosymbionts. Once prokaryotes and unicellular eukaryotes had colonized terrestrial ecosystems for millions of years, the first pluricellular animals appeared and radiated, thus inaugurating the Cambrian. The following sections constitute a collection of independent articles providing a general overview of these aspects.     

Fusion of airborne LiDAR and multispectral sensors reveals synergic capabilities in forest structure characterization

Forest stand structure is an important concept for ecology and planning in sustainable forest management. In this article, we consider that the incorporation of complementary multispectral information from optical sensors to Light Detection and Ranging (LiDAR) may be advantageous, especially through data fusion by back-projecting the LiDAR points onto the multispectral image. A multivariate data set of both LiDAR and multispectral metrics was related with a multivariate data set of stand structural variables measured in a Scots pine forest through canonical correlation analysis (CCA). Four statistically significant pairs of canonical variables were found, which explained 83.0% accumulated variance. The first pair of canonical variables related indicators of stand development, i.e. height and volume, with LiDAR height metrics. CCA also found attributes describing stand density to be related to LiDAR and spectral variables determining canopy coverage. Other canonical variables pertained to Lorenz curve-derived attributes, which are measures of within-stand tree size variability and heterogeneity, able to discriminate even-sized from uneven-sized stands. The most relevant result was to find that metrics derived from the multispectral sensor showed significant explanatory potential for the prediction of these structural attributes. Therefore, we concluded that metrics derived from the optical sensor have potential for complementing the information from the LiDAR sensor in describing structural properties of forest stands. We recommend the use of back-projecting for jointly exploiting the synergies of both sensors using similar types of metrics as they are customary in forestry applications of LiDAR.     

Cloud water interception in the high altitude tree heath forest (Erica arborea L.) of Paul da Serra Massif (Madeira,Portugal)

Cloud water interception (CWI) occurs when cloud droplets are blown against the forest canopy, where they are retained on the vegetation surface, forming larger water droplets that drip into the forest floor. CWI was measured from 1 October 1997 to 30 September 1999, on a first‐line tree heath (Erica arborea), at Bica da Cana, Madeira Island. Rainfall was corrected for wind‐loss effect and compared with throughfall and other climatological normals. The CWI depletion rate along a forest stand transect was also analysed during three distinct fog events in 2008. Cloud water was 28 mm day^?1, corresponding to 68% of total throughfall and 190% of the gross precipitation. Cloud water correlates directly with monthly normals of fog days and wind speed and correlates inversely with the monthly air temperature normal. CWI has an exponential correlation with monthly relative humidity normal. Cloud water capture depletion along the stand shows a logarithmic decrease. Although a forest stand does not directly relate to a first‐line tree heath, this study shows that CWI is a frequent phenomenon in the Paul da Serra massif. Restoration and protection of high altitude ecosystems in Madeira should be a priority, not only for biodiversity, ecological and economical purposes but also for its role in regional water resources. Copyright © 2011 John Wiley & Sons, Ltd.     

Specific profiles of polybrominated diphenylethers (PBDEs) and polychlorinated biphenyls (PCBs) in fish and tucuxi dolphins from the estuary of Paraíba do Sul River, Southeastern Brazil

Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) are ubiquitous pollutants in the environment and subject of several studies due to their adverse effects as endocrine disruptors. Despite the concern over widespread distribution of PBDEs even in sparsely populated regions of the world, few studies have reported their occurrence in Brazil. In this study, PBDEs and PCBs levels were measured in selected fish species and dolphins from Paraiba do Sul River, Brazil. BDE 47 and 85 were the predominant congeners. Low chlorinated congeners showed the highest concentrations in fish; however dolphins presented the highest proportion of PCBs 153, followed by 138 and 180. The daily intake of PBDEs and PCBs was estimated for the population of this region. PCB daily intake through consumption of croaker was above the limits set by the Brazilian Ministry of Agriculture, raising great concern over the existence of a source of PCB contamination in Brazil.     

Effect of extraction-method,period of incubation and tidal emersion on the viability of haemocytes from oysters

The impacts of pollution on marine organisms are often investigated using the viability of their haemocytes. Although this assay is routinely used in monitoring, field and laboratory experimentation, there has been less effort in further optimizing procedures to reduce artefacts and facilitate sampling over large geographic areas.     

Spartina densiflora demonstrates high tolerance to phenanthrene in soil and reduces it concentration

The present study was conducted to investigate the tolerance of Spartina densiflora to phenanthrene, and to test its ability in phenanthrene dissipation. A glasshouse experiment was designed to investigate the effect of phenanthrene from 0 to 1000 mg kg⁻¹ on growth and photosynthetic apparatus of S. densiflora by measuring chlorophyll fluorescence parameters, gas exchange and photosynthetic pigments. We also performed chemical analysis of plant samples, and determined the concentration of phenanthrene remaining in soil. S. densiflora survived to concentrations as high as 1000 mg kg⁻¹ phenanthrene in soil; in fact, there was no significant difference in RGR among the treatments after 30 days. Otherwise, phenanthrene affected photosynthetic apparatus at 100 and 1000 mg kg⁻¹; thus, the lower Φ_PSII could be explained by the declined photosynthetic pigment concentrations. Soil extraction indicated a more marked rate of phenanthrene disappearance in the soil in the presence of S. densiflora.     

Estimating quality factor from surface seismic data: A comparison of current approaches

The performances of the spectral ratio (SR), frequency centroid shift (FCS), and frequency peak shift (FPS) methods to estimate the effective quality factor Q are compared. These methods do not demand true amplitude data and their implementations were done following an “as simple as possible” approach to highlight their intrinsic potentials and limitations. We use synthetic zero-offset seismic data generated with a simple layer-cake isotropic model. The methods can be ranked from simple to complex in terms of automation as: FPS, FCS and SR. This is a consequence of: (i) peak identification consists basically of a sorting procedure, (ii) centroid estimation involves basically the evaluation of two well-behaved integrals, and (iii) implementation of the SR method involves at least choosing a usable frequency bandwidth and fitting a gradient. The methods can be ranked from robust to sensitive in the presence of noise content in the sequence SR, FCS, and FPS. This is consequence of: (i) the gradient estimate associated to the SR method averages out the noise content in the entire usable frequency bandwidth, (ii) in the presence of moderate-to-high noise level, the centroid estimation is biassed towards overestimating Q due to noise contribution in the tail of the amplitude spectrum, and (iii) peak identification is unstable due to local noise fluctuation in the amplitude spectrum around the peak frequency. Regarding the stability of the estimates relative to the attenuation amount, SR and FCS methods show similar behaviours, whereas FPS method presents an inferior performance. This fact is an indirect consequence of the sensitivity of FPS method to the noise content because the higher is the attenuation the lower is the signal-to-noise ratio. Finally, regarding the robustness of the methods to the presence of dipping layers, only SR and FCS methods provide good estimates, at least to typical dips in non-faulted sedimentary layers, with the estimates obtained with SR method being more accurate that those obtained with FCS method. Except in relation to the automation complexity, which is less important than the performances of the methods, SR method was superior or showed similar performance to FCS method in all scenarios we tried.     

Assessing homogeneous regions relative to drought class transitions using an ANOVA-like inference. Application to Alentejo, Portugal

In the present study, an ANOVA-like inference technique is used aiming at to assess if Alentejo, southern Portugal, could be considered a homogeneous region for drought management purposes. First, Alentejo was divided into four sub-regions according to latitude (north and south), and longitude (west and east). Inside each sub-region, 10 weather stations were considered. The time series of the Standardized Precipitation Index (SPI) were obtained for these stations using precipitation data for the period 1932–1999 (67 years). Contingency tables for the transitions between SPI drought classes were obtained for these time series. Loglinear models were fitted to these contingency tables to estimate the probabilities for drought class transitions. An ANOVA-like inference was applied considering the four sub-regions like treatments of a two way layout with two factors, latitude and longitude, each one with two levels, north and south, and west and east respectively. The weather stations of each sub-region were treated as replicates. Significant differences between west and east were found, that allowed to consider that Alentejo could be composed by two sub-regions.     

Inorganic Water Quality Monitoring Using Specific Conductance in Mexico

Five inorganic geochemical data sets with ion balances less than or equal to 5% (for different hydrogeologic basins in Mexico) were used to construct specific conductance (SC) vs. total dissolved solids (TDS) curves for each aquifer. These curves allow one to proxy the specific conductance for inorganic water quality. The data from these five curves were integrated to form a "global" curve for Mexico. The equation that defines the line is SC = 1.40 TDS + 18. We propose that, in addition to allowing ground water monitoring, establishing a SC vs. TDS curve may be implemented to monitor drinking water supplies such as wells and reservoirs. If anomalously high values are observed, this would warrant detailed geochemical sampling.     

Seismotectonics of Portugal and its adjacent area in the Atlantic

New elements on the seismicity of Portugal and new focal-mechanism solutions of earthquakes with epicentres situated off the coast of the Portuguese mainland and in the Azores region are presented. Historical seismicity data show that in the territory of the Portuguese mainland there are active faults that are responsible for earthquakes that have caused important damage and many casualties. However, most of the intraplate earthquakes with epicentres situated in the Portuguese mainland or near the shore are normally of small magnitude and this renders difficult their interpretation in the light of focal mechanisms. A solution for one earthquake, with magnitude 5 and epicentre at the Nazaré submarine canyon, is presented.Southwestwards of Cape St. Vincent there is an important seismic zone responsible for high-magnitude earthquakes such as that of 1 November 1755. This zone is situated in the region where the extension of the Messejana fault into the ocean joins with the Azores-Gibraltar fault.The seismicity of the area situated between the western coast of the Portuguese mainland and the Azores increases approximately along the 15°W meridian, from the latitude of the Azores-Gibraltar fault up to 44°N. Focal mechanisms of earthquakes with epicentres situated along this line show very similar solutions.The interpretation of the focal mechanism solutions of the earthquakes with epicentres situated in the studied area shows that the stress field trends approximately NW-SE. It is assumed that this stress field results from the interaction of the Eurasian and African plates; however, this direction is not maintained in the Azores region.