Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Distribution patterns of chlorophyll <Emphasis Type="Italic">a</Emphasis> in spring and autumn in association with hydrological features in the southern Yellow Sea and northern East China Sea

Two field studies were conducted to measure pigments in the Southern Yellow Sea (SYS) and the northern East China Sea (NECS) in April (spring) and September (autumn) to evaluate the distribution pattern of phytoplankton stock (Chl a concentration) and the impact of hydrological features such as water mass, mixing and tidal front on these patterns. The results indicated that the Chl a concentration was 2.43±2.64 (Mean ± SD) mg m^?3 in April (range, 0.35 to 17.02 mg m^?3) and 1.75±3.10 mg m^?3 in September (from 0.07 to 36.54 mg m^?3) in 2003. Additionally, four areas with higher Chl a concentrations were observed in the surface water in April, while two were observed in September, and these areas were located within or near the point at which different water masses converged (temperature front area). The distribution pattern of Chl a was generally consistent between onshore and offshore stations at different depths in April and September. Specifically, higher Chl a concentrations were observed along the coastal line in September, which consisted of a mixing area and a tidal front area, although the distributional pattern of Chl a concentrations varied along transects in April. The maximum Chl a concentration at each station was observed in the surface and subsurface layer (0–10 m) for onshore stations and the thermocline layer (10–30 m) for offshore stations in September, while the greatest concentrations were generally observed in surface and subsurface water (0–10 m) in April. The formation of the Chl a distributional pattern in the SYS and NECS and its relationship with possible influencing factors is also discussed. Although physical forces had a close relationship with Chl a distribution, more data are required to clearly and comprehensively elucidate the spatial pattern dynamics of Chl a in the SYS and NECS.     

The apparent copper complexing capacity of seawater and the interaction of copper with humic matter

The anodic stripping voltammetry with physically-coating mercury film electrode was used to investigate the complexing action of trace heavy metals in seawater with organic ligands. The apparent copper complexing capacity of seawater was determined by titrating the organic ligands in natural seawater with standard ionic copper solution. The complexing actions of copper in seawater with humic acid (HA) or fulvic acid (FA) were investigated by titrating copper in seawater with HA or FA solution. The equilibrium time, electrodeposition potential, and effect of pH etc. were investigated respectively. The results show that the interaction of copper in seawater with organic matter is a fast process. At natural pH, HA or FA tend to act with copper in seawater to form nonlabile complexes. During experimental electrode process, these complexes did not significantly dissociate. The experimental results were calculated according to 1∶1 complex formation.     

拉萨紫外辐射特征分析及估算公式的建立

本文利用2005~2010年的辐射观测资料对拉萨地区紫外辐射的时间变化特征及紫外辐射与总辐射比值的变化特征进行了分析,结果表明,紫外辐射与太阳总辐射的变化规律基本一致,日变化表现为正午大、早晚小;季变化特征是夏季6月最大,冬季1月最小.紫外辐射日累积值6年平均为0.87 MJ·m-2·d-1;紫外辐射有逐年递减的趋势.紫外辐射与总辐射比值也存在着明显日变化,表现为正午大、早晚小的规律;其季节变化也是夏季最大,冬季最小.紫外辐射与总辐射比值6年平均为0.0418;紫外辐射与总辐射比值也呈现逐年递减的趋势.利用2010年大气质量数和晴空指数,建立了适合于拉萨紫外辐射估量的公式,估算值的瞬时值与观测值的平均相对误差最大为8.66%,紫外辐射日累积重构值与观测值平均相对误差仅为5.5%.     

山东省近55年夏季降水和平均气温的多时间尺度分析

利用最近55a山东夏季降水和平均气温资料，统计分析了降水和平均气温的变化特征，并用Meyer小波变换方法，对山东省夏季降水和平均气温进行了分析，发现山东夏季降水呈明显下降趋势，气温略呈上升趋势。小波分析显示两者在中短周期上基本一致，即它们的中短周期基本在11a，5～6a和2～3a左右的时间尺度上变动。而这些基本周期正是太阳黑子活动和海气相互作用的周期，说明山东省夏季降水和平均气温的周期变化主要受太阳黑子活动和海-气相互作用的影响。     

乡土地理实践活动案例分析与应用——以“明光市女山古火山地质公园考察”为例

地理实践是地理科学的重要研究方法,也是中学地理学科的主要学习方式之一。中学地理实践活动应结合乡土地理资源,充分挖掘当地可供考察的自然、人文素材,形成丰富的乡土地理实践案例。本文以“女山古火山地质公园实践活动为案例”,探究乡土资源在地理实践活动中的应用。     

云南雨季逐候降水量的低频振荡特征

应用主分量方法分析了云南省84站1991~2000年雨季(5~10月)逐候降水量的主要时空特征,并用非整波技术分析了所提取的第1、第2主分量频谱分布的低频振荡特点,同时分析了低频振荡现象与El Nino(La Nina)事件及云南雨季降水多、少之间的关系。结果表明:①云南地区雨季降水主要低频振荡周期为6候(30天)、10候(50天)的月际振荡和15~17候(75~85天)的季节内振荡;②云南雨季的候降水每年都存在30天振荡周期,30天振荡是云南雨季固有的振荡;③当发生El Nino或La Nina异常气候事件时,云南雨季的候降水存在50天振荡周期;④当云南雨季存在75~85天振荡时,云南主汛期(6~8月)降水距平百分率为正(除2000年为零距平);当云南雨季不存在75~85天振荡时,云南主汛期降水距平百分率为负。     

高性能计算系统性能评测方法及其应用

为满足国家级气象数值模式业务系统对高性能计算资源不断增长的需求,提供规模更大、性能更优的高性能计算支撑平台,在高性能计算系统引进工作中开展了基于气象数值模式应用的高性能计算系统性能评测技术研究。该文将行业标准与实际业务应用相结合,设计了高性能计算系统性能评测方案,建立了中国气象局气象行业高性能计算系统性能评测模型。共选取10个测试程序,分别对单节点性能、网络性能和加速比等8个指标进行了测试和量化评分,并对主要测试结果中具有代表性的气象要素场进行了物理意义合理性检查。评测结果表明:评测方案设计合理,评测模型研究取得较好效果,保障了高性能计算系统引进工作顺利完成。     

升平油田葡萄花油层高分辨率层序地层格架及储层特征

利用高分辨率层序地层理论分析方法,在升平油田葡萄花油层三维地震资料及15口井岩心和356口井测井资料综合研究的基础上,通过不同级次基准面旋回界面特征的识别,将本区划分为1个长期、2个中期、6个短期和13个超短期层序,以超短期层序为目标,建立了单砂体等时对比单元的地层格架。以超短期层序为控制目标,详细研究了葡萄花油层沉积微相及单砂体分布规律:基准面上升期是三角洲河道砂体发育最有利时期,单砂体厚度大连续分布广;湖泛期细粒沉积物分布广泛,储集层不发育;基准面下降期受下切作用影响,河道砂体不发育。提出了葡萄花油层超短期旋回层序由于同沉积构造抬升作用所导致的从北到南逐渐减薄超覆尖灭新地层模式。     

金刚石压腔实验技术应用于生烃动力学研究浅析

金刚石压腔实验技术自发明以来就被广泛应用于高压领域的研究,成为高压下进行物质结构、性质等方面实验研究的重要方法。另一方面,生烃动力学近年来被广泛应用于含油气盆地烃源岩评价与勘探。文中对生烃动力学原理进行了介绍,对当前确定生烃动力学参数的热解实验装置进行评述,并对金刚石压腔实验技术应用于生烃动力学研究进行了分析。