应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

参照鱼类病毒性出血性败血症病毒序列,根据PCR引物设计的原则,设计巢式PCR引物,采用巢式逆转录聚合酶链反应(RT-PCR)方法,对如何快速检测鱼类病毒性出血性败血症病的方法进行了较为系统的研究,并对RT-PCR的反应条件进行了优化.结果表明,巢式RT-PCR扩增获得279bp的特异性片断,阴性对照无扩增条带.巢式RT-PCR扩增出的特异性片段经测序分析,结果证实与报道的序列完全一致.该方法最低可检测出0.1pg的鱼类病毒性出血性败血症病毒RNA.初步建立了VHSV的巢式RT-PCR检测方法,该方法灵敏、特异,可为VHSV的检测提供一个快速、有效的手段.     

Influence of Headland Breakwaters on Morphological Processes at Longfengtou Beach in Haitan Bay,Facing the Taiwan Strait

Breakwaters can be used as artificial headlands in beach nourishment to mitigate coastal erosion. Longfengtou Beach, located on the southwest coast of Haitan Bay facing the Taiwan Strait on the northeast, suffers severe erosion, where the monsoon causes significant season hydrodynamic variations. Headland breakwaters are intended to be employed to mitigate coastal erosion. A 2D sediment transport model is established using MIKE21 based on current-wave coupling and calibrated by measured data. Summer and winter wave conditions are chosen as characteristic weak and strong waves respectively. The numerical results of suspended sediment concentration and seasonal morphological evolution are compared under the conditions with and without the headland breakwaters. Sediment transport in summer can be regarded as mainly determined by current field, while in winter wave effect is strong enough to change current field. The northern breakwater, nearly perpendicular to the ebb current direction, obstructs the currents and decreases velocity of the ebb currents, and confines the water carrying sediments within the protection area during the flood period. The southern breakwater also breaks the waves in advance and significantly reduces the hydrodynamic effects during the flood period and maintains high suspended sediment concentration in the protection area. In general, two headland breakwaters decrease the erosion near the beach and enlarge the deposition area, which play a significant role in prevention of sediment loss in nearshore area and mitigate coastal erosion.     

云南区域数字地震台网的监测能力与限幅问题

采用地噪声包络幅度作为背景噪声的统计特征量,利用噪声峰值角频率处的地噪声速度振幅与对应的地噪声位移振幅Aμn的转换关系,把Av换算成近震震级公式中所需的位移量Aμ,在此基础上,计算了云南数字地震台网的监测能力,计算结果与实际观测结果相符.同时,分析了云南"九五"地震监测台网的大震限幅问题.     

新形势下黑龙江省对俄边境贸易的经济地理透视

本文从世界经贸格局变化和经济地理学角度探讨近两年来黑龙江省对俄罗斯边境地方经济贸易急剧下滑的原因，并立足于资源要素最佳配置的原则对其发展潜力进行了分析，最后提出了我国相应的发展对策。     

The Location of Office Space in the Metropolitan Service Economy of the United States, 1985–1990

As the economic base of most American metropolitan areas relies increasingly on services, office industries have shifted to a more pivotal role in the new metropolitan service economy. Using primary office-space usage data of the time period 1985–1990, the changing spatial patterns of this new metropolitan service economy are investigated. It is found that the primary office activities are predominantly concentrated in a relatively small number of the largest metropolitan areas. However, an emerging spatial dispersion in primary office activities is also exhibited. Such dispersion is extremely strong at the upper end of the urban hierarchy. The Sunbelt-Snowbelt dichotomy fails to reveal any differences in the growth pattern of the new metropolitan service economy. A more diversified growth pattern of primary office activities is observed. Concentrated dispersion is the major spatial characteristic of this new metropolitan service economy.     

SPOT图象的解析立体测图

本文讨论了适合于SPOT图象的用光束法平差解算外方位元素的数学模型;介绍了在国产解析测图仪APS—1上进行SOPT图象立体测图的方法;研究了SPOT投影方式的实时循环程序LOOP及SPOT图象的测图软件包。     

利用滚动式相关分析方法普查历史资料初探

介绍了一种新的普查历史资料的方法－滚动式相关分析方法，并将滚动相关分析法与一般相关分析法的相关分析结果进行了对比分析。结果表明，滚动式相关分析方法提高了相关稳定性，预报效果优于一般相关分析法。