Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

延边地层区晚石炭世地层

本文提出延边地层区的晚石炭世地层有两种类型：一是原地系统的天宝山组；二是外来岩块，包括原山秀岭组。并建议停止使用山秀岭组一名。     

Using 4C OBS to reveal the distribution and velocity attributes of gas hydrates at the northern continental slope of South China Sea

To investigate the distribution and velocity attributes of gas hydrates in the northern continental slope of South China Sea, Guangzhou Marine Geological Survey conducted four-component (4C) ocean-bottom seismometer (OBS) surveys. A case study is presented to show the results of acquiring and processing OBS data for detecting gas hydrates. Key processing steps such as repositioning, reorientation, PZ summation, and mirror imaging are discussed. Repositioning and reorientation find the correct location and direction of nodes. PZ summation matches P- and Z-components and sums them to separate upgoing and downgoing waves. Upgoing waves are used in conventional imaging, whereas downgoing waves are used in mirror imaging. Mirror imaging uses the energy of the receiver ghost reflection to improve the illumination of shallow structures, where gas hydrates and the associated bottom-simulating reflections (BSRs) are located. We developed a new method of velocity analysis using mirror imaging. The proposed method is based on velocity scanning and iterative prestack time migration. The final imaging results are promising. When combined with the derived velocity field, we can characterize the BSR and shallow structures; hence, we conclude that using 4C OBS can reveal the distribution and velocity attributes of gas hydrates.     

渤、黄海北部海冰年代时空变化特征分析

本文取上世纪后50年至2003年度冬季渤海地区气温作为与渤海冬季冰情有关的气候指标。分析得出,渤海地区冬季气温随着年代呈上升趋势,特别是80年代中期至本世纪初尤为明显。受其影响,渤海冬季的冰情时空分布状况发生了较大变化。其特点是:初冰日较多年平均值推后、终冰日较多年平均值提前,冰期较多年平均值偏短,冰厚较多年平均值偏薄,结冰范围(沿岸固定冰宽度和浮冰外缘线)较多年平均值偏小,甚至有些海区已多年未曾见到海冰了。     

武汉市区第四系含水层地下水有机污染敏感性研究

在详细调查武汉市水文地质条件和地下水污染现状的基础上，获得了高精度的武汉市水环境中微量有机污染物的组成数据。所检测出的有机组分达30余种，以苯及相关苯系物为主，污染程度较高的地下水主要分布在人口密集区和工业，商业区，应用改进的DRASTIC模型－地下水污染敏感性评价模型，在GIS平台上，编制了武汉市区地下水污染敏感性分区图。根据其评价结果，建议集中对那些敏感性相对较高的区域采取有效的环保措施，开发利用时应作出风险评价。     

日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

分潮波模型多资料数值计算方法研究 Ⅰ.岸边资料运动学外推的第一边值方法

对于含多资料三维分潮波模型建立了一种岸边资料运动学外推的潮波第一边值方法，并对渤海的四大原湖波进行试算。结果证实本方法可行，该方法使潮波方程既满足第一边值问题，充分利用岸边测站资料，又保证岸界法向流速为零条件，符合物理意义。并对复型病态矩阵的收敛问题提出了一种可行性处理。     

Variation in the vertical zonality of erodibility and critical shear stress of rill erosion in China's Hengduan Mountains

Soil erodibility is an essential parameter used in soil erosion prediction. This study selected the Liangshan town watershed to quantify variation in the vertical zonality of rill erodibility (k_r) in China's ecologically fragile Hengduan Mountains. Soil types comprised of yellow–brown (soil A), purple (soil B), and dry-red (soil C) in a descending order of occurrence from the summit to the valley, which roughly corresponds to the vertical climate zone (i.e. cool-high mountain, warm-low mountain, and dry-hot valley sections) of the study area. With elevation, vertical soil zonality varied in both soil organic matter (SOM) content and soil particle-size fractions. A series of rill erosion-based scour experiments were conducted, using water discharge rates of 100, 200, 300, 400, 500, and 600 mL min^-1. Additionally, detachment rates (Dr) were measured under three hydrological conditions (the drainage, saturation, and seepage treatments). Results show that both Dr and flow shear stress (Ʈ) values increased as discharge increased. As elevation increased, the k_r values decreased, while the vertical zonality of critical shear stress (Ʈ_c) values showed no obvious variation. The highest k_r values were observed during the seepage treatment, followed by the saturation treatment then drainage treatment, indicating that variation in vertical hydraulic gradients could significantly alter k_r values. This study also found that land-use types could also alter k_r and Ʈ_c values. Further research, however, is necessary to better quantify the effects of subsurface hydrological conditions and land-use types on k_r under different soil zonalities in China's Hengduan Mountains. © 2018 John Wiley & Sons, Ltd.     

Mechanical behavior and micro-structure of cement kiln dust-stabilized expensive soil

In this study, the effects of cement kiln dust (CKD) on the swelling properties, strength properties, and microstructures of CKD-stabilized expansive soil were investigated. Samples were prepared and stabilized with different CKD content ratios, ranging from 0 to 18% by dry mass. The results obtained show that the maximum swelling pressures decrease exponentially with increases in CKD content. Both the cohesion and unconfined compressive strength (UCS) increase at ratios below 10% CKD and then decrease slightly, above that ratio. CKD can also improve the strength of saturated, expansive soil. There is no visible change of UCS for soil without CKD when cured, while the UCS of a sample with 10% CKD content after curing for 90 days is higher than that after curing for only 1 day. This indicates that CKD can improve the long-term strength of expansive soil. Finally, microstructure analysis reveals that the addition of CKD reduces the montmorillonite content of expansive soil and decreases its swelling properties. The addition of CKD also changes the pore volume distribution, both the size and amount of macro-pores and micro-pores decrease with increase in CKD content. For saturated samples, the size of macro-pores is obviously reduced, while that of micro-pores is slightly increased for both treated and untreated soils. Hydration and saturation processes make the soil structure become dispersive which results in a lower strength, and adding CKD can restrain this process. The suggested optimal CKD content is between 10 and 14% and with a curing time of more than 27 days.