Ultraviolet Laser Sampling and High Resolution Inductively Coupled Plasma-Mass Spectrometry of NIST and BCR-2G Glass Reference Materials

A Merchantek LUV266 ^TM petrographic ultraviolet (UV) laser microprobe has been used in conjunction with a Finnigan MAT ELEMENT ^TM high resolution ICP double-focusing mass spectrometer (HR-ICP-MS) for solid microsampling and geochemical analysis. This new configuration for laser ablation has the advantage of coupling the high sensitivity and fast scanning technology applied in the ELEMENT with the ablation efficiency of a UV laser. Optimisation of the configuration on the synthetic NIST SRM 612 glass standard reference material using Q-switched UV laser energy of 2 mJ and a 5 Hz repetition rate demonstrates: (1) a linear element response factor throughout the mass spectrum, suggesting limited fractionation during laser ablation; (2) a high sensitivity and very low background noise for most elements of interest; (3) limits of detection ranging from 3 ng g-1 for Sc to 300 pg g-1 for U; (4) a flat topped peak shape suitable for precise isotopic measurements; and (5) a flat bottomed crater geometry which permits progressive ablation for the analysis of thin sections. Average precision and accuracy estimates based on replicate analyses of synthetic NIST standard reference material and USGS BCR-2G glasses are below 10% for most elements.     

FRACTURE CHARACTERIZATION FROM NEAR-OFFSET VSP INVERSION

A global optimization method incorporating a ray-tracing scheme is used to invert observations of shear-wave splitting from two near-offset VSPs recorded at the Conoco Borehole Test Facility, Kay County, Oklahoma. Inversion results suggest that the seismic anisotropy is due to a non-vertical fracture system. This interpretation is constrained by the VSP acquisition geometry for which two sources are employed along near diametrically opposite azimuths about the well heads. A correlation is noted between the time-delay variations between the fast and slow split shear waves and the sandstone formations.     

Characterization of grass shrimp (Palaemonetes pugio) embryo toxicity tests using the water soluble fraction of number 2 fuel oil

Toxicity test procedures using grass shrimp Palaemonetes pugio exposed to water soluble fraction (WSF) of Number 2 fuel oil were modified to improve test utility and efficiency. The original test procedure, a 12-day embryo exposure period in glass tubes, was compared with modified 4-day and 12-day tests in plastic tissue culture plates. Comparison of LC₅₀ values, coefficients of variation and time to hatch indicated no significant change in test sensitivity due to the plastic containers. Sensitivity was slightly reduced by shortening the test to a 4-day exposure period initiated 9 days after oviposition. This exposure period encompassed sensitive life stages of the developing organism. Using the 4-day exposure in plastic plates, variability due to different broods and preparations of WSF was characterized.     

Effects of dimilin on the blue crab,Callinectes sapidus,in shallow-water habitats

Diffubenzuron (Dimilin) is used as an insecticide for control of the gypsy moth. Because it is a chitin synthetase inhibitor, it can be a potential threat to other arthropods, including crustaceans. We used a static renewal testing paradigm to determine the LC₅₀ of Dimilin WP-25 to juvenile blue crabs (carapace width: 25–60 mm). Both molt stage of dose frequency effect toxicity. When we exposed the crabs at random molt stages, LC₅₀=3.5 mg l^?1. When crabs were exposed on the day of molt, LC₅₀=300 μg l^?1. If initial exposure occurred on the day of molt and the crabs were subsequently exposed to repeated doses, LC₅₀=18.5 μg l^?1. Effects were age and molt-stage sensitive. We include a review of the data available on Dimilin effects in the marine and estuarine environments.     

Occurrence of IRGAROL 1051 in coastal waters from Biscayne Bay,Florida, USA

Surface water samples from marinas, commercial ports and open bay areas collected from Biscayne Bay and the Miami River, Florida, USA, were analyzed for the occurrence of IRGAROL 1051 by GC/MS. The anifouling boosting herbicide was found in 80% (46/57) of the samples collected between March 1999 and September 2000. Concentrations within the bay range between non-detected (<1 ppt) and 61 ppt (ng/L) and were generally low compared with levels reported in European or Japanese waters. Aside from the elevated concentrations observed along the Miami River South Fork (61 ppt), the highest concentrations observed in the bay corresponded to marinas with high density of pleasure craft and restricted water circulation. In contrast, occurrence of IRGAROL 1051 along the commercial port or the cruise line terminal was generally lower (<1-2.2 ppt). Concentrations around Coconut Grove Marina were consistently higher (5-12 ppt) than the rest of the bay waters during the whole period of time surveyed.     

A Preliminary Survey of the Nitrogen and Carbon Isotope Characteristics of Fish from the Lagoons of Egypt’s Nile Delta

The present study reports nitrogen and carbon stable isotope data (δ¹⁵N and δ¹³C) from four large (63–400 km²), shallow (∼1 m) coastal lagoons on Egypt’s Nile Delta. While the lagoons all receive sewage and agricultural drainage, the magnitude of loading varies. In this preliminary survey, we document wide variability in the δ¹⁵N and δ¹³C isotope values of major fish groups among these lagoons. There were no consistent or significant differences among the major groups of fish, including carp, catfish, mullet, and tilapia. There was a strong positive correlation (R ² = 0.84) between the average δ¹⁵N values of fish muscle and estimated water residence time among the lagoons. This preliminary evidence suggests that nitrogen cycle transformations may be more important than primary N source differences in determining N isotopic ratios of organisms in the lagoons. The δ¹³C results point to the probable importance of autochthonous particulate organic matter rather than terrestrial detritus or marine plankton in the diets of resident fish populations in the lagoons.     

Ecological Signatures of Anthropogenically Altered Tidal Exchange in Estuarine Ecosystems

One of the most conspicuous anthropogenic disturbances to estuaries worldwide has been the alteration of freshwater and tidal influence through the construction of water control structures (dikes, tide gates, culverts). Few studies have rigorously compared the responses of differing groups of organisms that serve as contrasting conservation targets to such anthropogenic disturbances in estuarine ecosystems. Elkhorn Slough in central California includes a spectrum of tidally restricted habitats behind water control structures and habitats experiencing full tidal exchange. To assess community composition for several different taxa in habitats with varying tidal exchange, we employed a variety of field approaches and synthesized results from several different studies. Overall, we found that communities at sites with moderately restricted tidal exchange were fairly similar to those with full tidal exchange, but those with extremely restricted tidal exchange were markedly different from other categories. These differences in community composition are likely the result of several factors, including restricted movement due to physical barriers, differences in water quality characteristics, and differences in habitat structure. Indeed, in this study, we found that water quality characteristics strongly vary with tidal restriction and may strongly influence patterns of species presence or absence. We also found that different conservation targets showed contrasting responses to variation in tidal exchange. Full exchange appears to favor native oysters, commercially valuable flatfish, migratory shorebirds, and site-level biodiversity. Minimal tidal exchange due to water control structures supports a suite of estuarine endemics (including the tidewater goby and California brackish snail) not represented elsewhere and minimizes invasions by non-native marine species. Altogether, our results suggest that total estuary-wide biodiversity may be enhanced with a mosaic of tidal exchange regimes.     

A facies interpretation of the eruption and emplacement mechanisms of the upper part of the Neapolitan Yellow Tuff,Campi Flegrei,southern Italy

This study focuses on the upper part, Member B, of the Neapolitan Yellow Tuff (NYT). Detailed measurements of stratigraphic sections within the unlithified pozzolana facies show that Member B is composed of at least six distinct depositional units which each record a complex fluctuation between different styles of deposition from pyroclastic density flows. Six lithofacies have been identified: (1) massive valleyponded facies, the product of non-turbulent flows; (2) inverse-graded facies formed by flows that were turbulent for the majority of transport but were deposited through a non-tubulent basal regime; (3) regressive sand-wave facies, the product of high-concentration, turbulent flows; (4) stratified facies, the product of deposition from turbulent, low-particle-concentration, flows; (5) particle aggregate and (6) vesicular ash lithofacies, both of which are considered to have formed by deposition from turbulent, low-concentration flows. Although the whole eruption may have been phreatomagmatic, facies 1–4 are interpreted to be the product of dry eruptive activity, whereas facies 5 and 6 are considered to be of wet phreatomagmatic eruptive phases. Small-scale horizontal variations between facies include inverse-graded lithofacies that pass laterally into regressive sand-wave structures and stratified deposits. This indicates rapid transition from non-turbulent to turbulent deposition within the same flow. Thin vesicular ash and particle aggregate layers pass laterally into massive valley-ponded vesicular lithofacies, suggesting contemporaneous wet pyroclastic surges and cohesive mud flows. Three common vertical facies relations were recognised. (1) Massive valley-ponded and inverse-graded facies are overlain by stratified facies, suggesting decreasing particle concentration with time during passage of a flow. (2) Repeated vertical gradation from massive up into stratified facies and back into massive beds, is indicative of flow fluctuating between non-turbulent and turbulent depositional conditions. (3) Vertical alternation between particle aggregates and vesicular facies is interpreted as the product of many flow pulses, each of which involved deposition of a single particle aggregate and vesicular ash layer. It is possible that the different facies record stages in a continuum of flow processes. The deposits formed are dependent on the presence, thickness and behaviour of a high-concentration, non-turbulent boundary layer at the base of the flow. The end members of this process are (a) flows that transported and deposited material from a non-turbulent flow regime and (b) flows that transported and deposited material from a turbulent flow regime.