鄂霍次克海冷泉沉积物真核生物多样性的初步研究

从鄂霍次克海沉积物样品中提取总基因组DNA,进行核糖体rRNA基因内转录间隔区(ITS)的扩增,构建克隆文库并进行测序,对该环境下真核生物的多样性进行了初步研究。结果发现,该环境下的真核生物具有较高的多样性,主要为3大类,真核藻类、真菌和微型浮游动物。藻类和微型浮游动物的组成与前人在该海域浮游生物生态学的研究相吻合,而真菌群落具有较高的多样性,这为该区域真核生物多样性、所参与的生物地球化学过程及生态学意义的研究奠定了基础。     

海洋链霉菌M506 的鉴定及代谢产物抑菌活性初步研究

自胶州湾沉积物中分离出海洋链霉菌M506,根据其形态特征观察、生理生化特征试验及基于16S rDNA序列系统进化分析,初步鉴定该菌为灰略红链霉菌.采用不同培养条件对其进行小规模发酵,利用金黄色葡萄球菌(Staphylococcus aureus),大肠杆菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis)和苏云金芽孢杆菌(Bacillus thuringiensis)四株细菌作为指示菌,检测其抑菌活性.结果表明,该菌株代谢产物粗提物对于金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌等具有显著抑制活性.     

Sea surface height variations in the Mindanao Dome region in response to the northern tropical Pacific winds

Sea surface height (SSH) variability in the Mindanao Dome (MD) region is found to be one of the strong variations in the northern Pacific. It is only weaker than that in the Kuroshio Extension area, and is comparable to that in the North Pacific Subtropical Countercurrent region. Based on a 1.5-layer reduced gravity model, we analyzed SSH variations in this region and their responses to northern tropical Pacific winds. The average SSH anomaly in the region varies mainly on a seasonal scale, with significant periods of 0.5 and 1 year, ENSO time scale2-7years, and time scale in excess of 8 years. Annual and long-term variabilities are comparably stronger. These variations are essentially a response to the northern tropical Pacific winds. On seasonal and ENSO time scales, they are mainly caused by wind anomalies east of the region, which generate westward-propagating, long Rossby waves. On time scales longer than 8 years, they are mostly induced by local Ekman pumping. Long-term SSH variations in the MD region and their responses to local winds are examined and discussed for the first time .     

Changes in the small-jellyfish community in recent decades in Jiaozhou Bay, China

We used long term monitoring data to evaluate changes in abundance and species dominance of small-jellyfish (collected with zooplankton net whose bell diameter was less than 5 cm) between 1991 and 2009 in the Jiaozhou Bay, China. Zooplankton samples were vertically towed with conical plankton net from near-bottom to surface, identified microscopically, and mapped in time-space using Grapher 7.0 and Surfer 8.0. Results show that the abundance of small-jellyfish throughout the bay had been increasing during 2001-2009 on average of 15.2 ind./m 3 , almost 5 times higher than that between 1991 and 2000. The occurrence of peak abundance shifted from spring to summer after 2000, and two peaks appeared in spring and summer, respectively, after 2005. Both the abundance and the frequency of blooms of small-jellyfish increased after 2000 in the bay. In addition, the biodiversity of jellyfish has increased significantly in recent years with a change in dominant species. Several new dominant species appeared after 2000, including Rathkae octopunctata in winter, Phialidium hemisphaericum in spring, summer, and autumn, Phialucium carolinae in spring, and Pleurobrachia globosa in summer and autumn, while some previous dominant species throughout the 1990s (Eirene ceylonensis, Zanclea costata, Lovenella assimilis, and Muggiaea atlantica) were no longer dominant after 2000. The abundance of small-jellyfish was positively correlated with the density of dinoflagellates, and the abundance of zooplankton. We believe that the changes in smalljellyfish abundance and species composition were the result of eutrophication, aquaculture and coastal construction activities around the bay. Concurrently, seawater warming and salinity decrease in recent decades promoted the growth and reproduction of small-jellyfish in the bay.     

A dinoflagellate <Emphasis Type="Italic">Cochlodinium geminatum</Emphasis> bloom in the Zhujiang (Pearl) River estuary in autumn 2009

A severe Cochlodinium geminatum red tide (>300 km2) was observed in the Zhujiang (Pearl) River estuary, South China Sea in autumn 2009. We evaluated the environmental conditions and phytoplankton community structure during the outbreak. The red tide water mass had significantly higher dissolved inorganic phosphate (DIP), ammonia, and temperature, but significantly lower nitrite, nitrate, dissolved inorganic nitrogen (DIN), and DIN/DIP relative to the non-red-tide zones. The phytoplankton assemblage was dominated by dinoflagellates and diatoms during the red tide. C. geminatum was the most abundant species, with a peak density of 4.13×107 cell/L, accounting for >65% of the total phytoplankton density. The DIN/DIP ratio was the most important predictor of species, accounting for 12.45% of the total variation in the phytoplankton community. Heavy phosphorus loading, low precipitation, and severe saline intrusion were likely responsible for the bloom of C. geminatum.     

南海北部陆缘构造属性研究进展

南海北部陆缘位于特提斯与古太平洋两大构造域的叠合部位,构造特征十分复杂,其构造属性一直是国内外学者争论的焦点,从主动陆缘到被动陆缘,火山型被动陆缘到非火山型被动陆缘等均有表述。南海复杂的形成机制以及东、西部构造差异性所引起的地球物理、岩浆活动等认识的异同,是造成南海北部陆缘构造属性认识差异的主要原因。通过与全球典型地区的比较研究,进一步加强对南海形成演化过程分析,开展大洋钻探与多学科综合分析,揭示南海海盆的多期扩张与多盆张裂特征,是认识南海北部陆缘构造属性的关键。探讨了南海三叉裂谷张裂模式,初步认为南海第1次扩张具有非火山型被动陆缘性质,第2次扩张具有火山型陆缘性质。     

Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus, and compared the response of screened and unscreened (control) P. trituberculatus challenged with V. alginolyticus. We measured superoxide dismutase, catalase, acid phosphatase, alkaline phosphatase, and peroxidase activity and the content of hemocyanin in the plasma and phenoloxidase activity in serum. The cumulative survival rate after 24-h challenge with V. alginolyticus was significantly higher in the screened crabs than in the unscreened crabs (P<0.05). T-SOD and PO activity were significantly lower in the screened stock than in the unscreened stock (P<0.05). POD, CAT, and ACP activity and hemocyanin content were significantly higher in the screened stock than in the unscreened stock. Our results suggest that the screened stock was more resistant to infection. Furthermore, the indices we measured may be used to evaluate the health state of P. trituberculatus.     

Agricultural land use optimal allocation system in developing area: Application to Yili watershed, Xinjiang Region

In developing countries, land productivity involves little market, where the agricultural land use is mainly determined by the food demands as well as the land suitability. The land use pattern will not ensure everywhere enough land for certain cropping if spatial allocation just according to land use suitability. To solve this problem, a subzone and a pre-allocation for each land use are added in spatial allocation module, and land use suitability and area optimization module are incorporated to constitute a whole agricultural land use optimal allocation (ALUOA) system. The system is developed on the platform .Net 2005 using ArcGIS Engine (version 9.2) and C# language, and is tested and validated in Yili watershed of Xinjiang Region on the newly reclaimed area. In the case study, with the help of soil data obtained from 69 points sampled in the fieldwork in 2008, main river data supplied by the Department of Water Resources of Xinjiang Uygur Autonomous Region in China, and temperature data provided by Data Center for Resources and Environmental Sciences, Chinese Academy of Sciences, land use suitability on eight common crops are evaluated one by one using linear weighted summation method in the land use suitability model. The linear programming (LP) model in area optimization model succeeds to give out land area target of each crop under three scenarios. At last, the land use targets are allotted in space both with a six subzone file and without a subzone file. The results show that the land use maps with a subzone not only ensure every part has enough land for every crop, but also gives a more fragmental land use pattern, with about 87.99% and 135.92% more patches than the one without, while at the expense of loss between 15.30% and 19.53% in the overall suitability at the same time.     

RTK技术在老挝甘蒙省钾盐矿勘查区地形图修测中的应用

以老挝人民民主共和国甘蒙省钾盐矿勘查区1∶50000地形图修测项目实例,介绍GPS RTK技术的基本原理和工作流程,总结出这种技术的优势所在以及实践操作中应该注意到的问题。