Molecular expression of opsin gene in growing juvenile mackerel (<Emphasis Type="Italic">Scomber japonicus</Emphasis> Houttuyn)

Fish have developed color vision that is closely adapted to their photic environments, where both spectral sensitivity and the number of visual opsins are influenced. The mackerel used in this study is one of the most important fishery stocks in Korea. The opsin gene of the mackerel juveniles after 20 days in hatching was isolated and characterized based on the molecular study of visual photoreceptor. The full-length mackerel opsin gene was obtained by PCR amplification of genomic DNA, as well as cDNA synthesis. Sequence analysis of the opsin gene showed that it contained a 1,080 bp open reading frame encoding 360 amino acids. Based on Schiff’s base formation (S114, K119), glycosylation (E3, F37) and palmitoylation (S281, 282), the deduced amino acid sequence had a typical rod opsin. The mackerel and Gempylus serpens showed 73.7% DNA homology on opsin gene, which was higher than any other of investigated species. In the analysis of phylogenetic relationship, the genetic placement of the mackerel is closer to that of Scombroidei than Labroidei, with supporting somewhat strong bootstrap value. In the analysis of Northern and RT-PCR, the probed products were observed only in rapidly growing juveniles. These findings indicate that in mackerel opsin mRNA expression can be detected in day-20 hatching larvae. It may play an important role in stimulating growth hormone.     

Estimation of the Roll Hydrodynamic Moment Model of a Ship by Using the System Identification Method and the Free Running Model Test

When a fast container ship or a naval vessel turns, accompanying roll motions occur. This roll effect must be considered in the horizontal equations of the motion of the ship to predict the maneuverability of the ship properly. In this paper, a new method for determining a model structure of the hydrodynamic roll moment acting on a ship and for estimating the hydrodynamic coefficients is proposed. The method utilizes a system identification technique with the data from sea trial tests or from free running model (FRM) tests. To obtain motion data that is applied to the proposed algorithm, an FRM of a large container ship was developed. Using this model ship, standard maneuvering tests were carried out on a small body of water out of doors. A hydrodynamic roll moment model was constructed utilizing the data from turning circle tests and a 20-20 zig-zag test. This was then confirmed through a 10-10 zig-zag test. It was concluded that a model structure of the hydrodynamic roll moment model could be established without difficulty through a system identification method and FRM tests.     

Performance analysis of digital acoustic communication in a shallowwater channel

The major obstacle to underwater acoustic communication is the interference of multi-path signals due to surface and bottom reflections. High speed acoustic transmission over a shallow water channel characterized by small grazing angles presents formidable difficulties. The reflection losses associated with such small angles are low, causing large amplitudes in multi-path signals. In this paper we propose a simple but effective model for multi-path interference, which is then used to assess the performance of a digital communication system operating in a shallow water channel. The results indicate that transmission rates in excess of 8 kbits/s are possible over a distance of 13 km and channel depth of only 20 meters. Such a system offers improved performance in applications such as data collection from underwater sensors     

Acoustic bubble counting technique using sound speed extracted fromsound attenuation

Sound attenuation has been solely used to estimate bubble size distributions of bubbly water in the conventional acoustic bubble sizing methods. These conventional methods are useful for the void fraction around 10^-6 or lower. However, the change of compressibility in the bubbly water also should be considered in bubble sizing for the void fraction around 10^-5 or higher. Recently the sound speed as well as sound attenuation was considered for acoustic bubble size estimation in bubbly water. In this paper, the sound speed estimated from sound attenuation in bubbly water by an iterative method is used for a bubble counting. This new iterative inverse bubble sizing technique is numerically tested for bubble distributions of single-size Gaussian, and power-law functions. The numerical simulation results are in agreement with the given bubble distributions even for the high void fractions of 10^-4-10^-3. It suggests that the iterative inverse technique can be a very powerful tool for practical use in acoustic bubble counting in the ocean     

Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes

To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.     

Stability Study of a Tide Embankment Subjected to Sea Level Variations Using Centrifugal Model Tests

To investigate the behavior of dredged-sea-sand fill compacted inside tide embankments with a damaged geosynthetic mat, centrifugal model tests and numerical simulation were conducted, both considering variations in sea level. The results from the three centrifugal model tests demonstrate that the subsidence of the dredged-sea-sand fill inside tide embankments with a damaged geosynthetic mat was strongly affected by the loss of dredged-sea-sand into the filter layers with large particles and a decrease in the bearing capacity of the filter layers with small particles. In addition, a comparison of the test and simulation results confirms that the loss of sand into the filter layer and the subsidence of the dredged-sea-sand fill were well reproduced by the numerical simulation.     

Forward induced seismic hazard assessment: application to a synthetic seismicity catalogue from hydraulic stimulation modelling

The M _w 3.2-induced seismic event in 2006 due to fluid injection at the Basel geothermal site in Switzerland was the starting point for an ongoing discussion in Europe on the potential risk of hydraulic stimulation in general. In particular, further development of mitigation strategies of induced seismic events of economic concern became a hot topic in geosciences and geoengineering. Here, we present a workflow to assess the hazard of induced seismicity in terms of occurrence rate of induced seismic events. The workflow is called Forward Induced Seismic Hazard Assessment (FISHA) as it combines the results of forward hydromechanical-numerical models with methods of time-dependent probabilistic seismic hazard assessment. To exemplify FISHA, we use simulations of four different fluid injection types with various injection parameters, i.e. injection rate, duration and style of injection. The hydromechanical-numerical model applied in this study represents a geothermal reservoir with preexisting fractures where a routine of viscous fluid flow in porous media is implemented from which flow and pressure driven failures of rock matrix and preexisting fractures are simulated, and corresponding seismic moment magnitudes are computed. The resulting synthetic catalogues of induced seismicity, including event location, occurrence time and magnitude, are used to calibrate the magnitude completeness M _c and the parameters a and b of the frequency-magnitude relation. These are used to estimate the time-dependent occurrence rate of induced seismic events for each fluid injection scenario. In contrast to other mitigation strategies that rely on real-time data or already obtained catalogues, we can perform various synthetic experiments with the same initial conditions. Thus, the advantage of FISHA is that it can quantify hazard from numerical experiments and recommend a priori a stimulation type that lowers the occurrence rate of induced seismic events. The FISHA workflow is rather general and not limited to the hydromechanical-numerical model used in this study and can therefore be applied to other fluid injection models.     

Inclined lidar observations of boundary layer aerosol particles above the Kongsfjord,Svalbard

An inclined lidar with vertical resolution of 0.4 m was used for detailed boundary layer studies and to link observations at Zeppelin Mountain (474 m) and Ny-Ålesund, Svalbard. We report on the observation of aerosol layers directly above the Kongsfjord. On 29 April 2007, a layer of enhanced backscatter was observed in the lowest 25 m above the open water surface. The low depolarization ratio indicated spherical particles. In the afternoon, this layer disappeared. The ultrafine particle concentration at Zeppelin and Corbel station (close to the Kongsfjord) was low. On 1 May 2007, a drying process in the boundary layer was observed. In the morning, the atmosphere up to Zeppelin Mountain showed enhanced values of the backscatter coefficient. Around noon, the top of the highly reflecting boundary layer decreased from 350 to 250 m. The top of the boundary layer observed by lidar was confirmed by radiosonde data.     

Reconstruction of historical nutrient levels in Korean and Japanese coastal areas based on dinoflagellate cyst assemblages

Dinoflagellate cysts acquired from sediment cores were analyzed in order to reconstruct historical nutrient levels in Gamak Bay, Korea and Ariake Bay, Japan. Dinoflagellate cyst assemblages in Gamak Bay were characterized by high proportions of heterotrophic cysts such as Brigantedinium spp., Protoperidinium americanum and Polykrikos cysts, which suggested that nutrients levels may have already been high before 1970s, and then increased further to the hypertrophic conditions of the 1990s. In contrast, dinoflagellate cyst assemblages in Ariake Bay were characterized by high relative abundances of Lingulodinium machaerophorum and Spiniferites spp., which suggested that nutrient levels in Ariake Bay had increased gradually since the mid 1960s, and may have been significantly enhanced by the mid 1980s. Dinoflagellate cyst assemblages reflecting environmental changes in the two bays are contrasting, perhaps due to different nutrient enrichment mechanisms. This suggests that the indicators of nutrient levels encoded in dinoflagellate cyst assemblages may exhibit site-specific information.     

Conservation of Korean Rural Heritage through the Use of Ecomuseums

Rural heritage that involves agricultural heritage could be critical to enhancing community identity through a conservational approach. The purpose of this research is to develop conservational methods for rural heritage that include agricultural heritage areas through the use of ecomuseums. To conduct the research, we first reviewed the concepts of ecomuseum and rural heritage. Secondly, we defined the concept of the Korean rural ecomuseum by taking into consideration the concept defined by Riviere, Globally Important Agricultural Heritage Systems (GIAHS), the rural heritage policy of Europe, and the agricultural heritage systems of Korea. Thirdly, we also describe methods that are applicable to Korean rural ecomuseums, which include type classification and components. Finally, we applied the ecomuseum concept to two pilot models in the Cheongsando and Gurae areas, both Korea Important Agricultural Heritage System (KIAHS) sites. We conclude that it is desirable to expand the range of targets for conservation from agricultural heritage to rural heritage areas. From this point of view, the rural ecomuseum can be a highly valuable tool that can help preserve agricultural heritage sites and neighboring areas. Furthermore, the ecomuseum can enhance community identities, and strengthen local economies.