CCC plasmid was isolated from an economically important blue-green alga- Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F, strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the OOC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid isolation, especially for filamentous blue-green algae. 相似文献
Since Ramsay and Graham (1970 ) system atically deliv-ered study results of ductile shear zone,the study on shearzone has been put forward rapidly and has obtained greatachievements.After the“gold- bearing shear zone” type ofmetallogenic model proposed by Bonnemaison(1986 ) ,lots ofstudies showed that large gold- bearing shear zone system,which underwent multi- stage and multi- level tectonic- m inerl-ization evolution,is a complex deformational metam orphic ge-ological body with different d… 相似文献
Rainfall-induced slope instabilities are ubiquitous in nature, but simulation of this type of hazards with centrifuge modelling still poses difficulties. In this paper, we introduce a rainfall device for initiating slope failure in a medium-sized centrifuge. This rainfall system is simple, robust and affordable. An array of perforated hoses is placed close above the model slope surface to generate the raindrops. The rainfall intensity depends on the centrifuge acceleration and the flow rate of the water supply, which is controlled by the size and number of the tiny pinholes in the hose walls. The rainfall intensities that are tested range from 2.5–30 mm/h, covering the intensity range of moderate, heavy and torrential rainfall events. Our model test with rainfall-induced slope failure shows that this system is capable of generating relatively uniform rainfall of wide intensities and leads to various patterns of slope failure.