The signal excited by the airgun source is weak, and is easily affected by human motion noise, instrument failure, and earthquake and blasting events, resulting in insufficient utilization of the amplitude information of the airgun signal. The effective removal of interference and the preservation of true amplitude information of the signal is difficult in airgun signal processing. Based on the randomness of noise and the highly repetitive characteristics of airgun signals, we propose probability data screening method. The airgun data from experiments conducted in the Ansha Reservoir and Shanmei Reservoir in Fujian Province in June, 2017 are taken as the research object to assess the effect in practical applications. The results show that the probability data screening method can automatically reject multiple interferences and effectively preserve the amplitude information of the signal by screening out large amplitude records in appropriate proportions. Compared with direct linear superposition, the probability-related linear superposition method can effectively reduce the impact of abnormal interference, and significantly improve the quality of the observed data. 相似文献
The appropriate reference gene is a prerequisite for accurate normalization of gene expression level, and research on suitable reference genes in clam Cyclina sinensis is scarce. To improve the situation, we selected five commonly used housekeeping genes, including β-actin, Elongation factor 1-α (EF1-α), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 40S ribosomal protein S18 (RPS18), and Tubulin α (TUB-α), then evaluated their expression stability in different adult tissues and under different experimental treatments (salinity stress and Vibrio parahaemolyticus infection). Their expression stability was analyzed by three frequently used programs, geNorm, NormFinder, and BestKeeper. This analysis indicated that multiple genes should be used for normalization, and we concluded that the reference gene combination GAPDH-RPS18-β-actin, should be used for qRT-PCR analysis in different tissues of C. sinensis under normal physiological conditions. For the clams under salinity stress and Vibrio infection, EF1-α-GAPDH-RPS18 was recommended as the gene combination for qRT-PCR normalization. TUB-α was generally poorly ranked by all programs, and should not be used in future studies. This study should provide fundamental support for accurate quantitative gene expression analysis of this species.