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231.
对虾一种无包涵体杆状病毒病原的PCR检测 总被引:4,自引:0,他引:4
为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义. 相似文献
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叙述了目前海水现场水色测量的弊端 ,指出数字化海洋水色的潜在应用价值 ;根据色度学及色度学的三原色基本理论 ,确定海洋现场水色的色度要素 ,使海洋现场水色标号统一为物理学中严格的颜色物理量。结合 1 996年渤海水域光谱反射测量 ,对渤海水域 4个典型站位的反射光谱做了水色色度分析 ,并进行了 2种测量方法的比较 相似文献
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关于波浪Boussinesq方程的研究 总被引:2,自引:0,他引:2
对有关波浪 Boussinesq方程的研究成果进行了系统的归纳总结和评述 ,以期对本学科的发展起到一定的引导和促进作用 相似文献
237.
Measurement of nuclides of uranium and thorium series of disequilibrium using γ-spectroscopy 总被引:3,自引:0,他引:3
INTRODUCTIONTheradionuclidesofnaturaluraniumandthoriumseriescanbeusedtotracemarinepro cessofvarioustemporalandspatialscaleandpropertiesthatprovideplentifulinformationaboutphysics,chemistry ,geoscienceandbiologyofoceanbecausetheyhavedifferentchemicalpro… 相似文献
238.
Estimation of Rapidly Time-Varying Sparse Channels 总被引:2,自引:0,他引:2
The estimation of sparse shallow-water acoustic communication channels and the impact of estimation performance on the equalization of phase coherent communication signals are investigated. Given sufficiently wide transmission bandwidth, the impulse response of the shallow-water acoustic channel is often sparse as the multipath arrivals become resolvable. In the presence of significant surface waves, the multipath arrivals associated with surface scattering fluctuate rapidly over time, in the sense that the complex gain, the arrival time, and the Dopplers of each arrival all change dynamically. A sparse channel estimation technique is developed based on the delay-Doppler-spread function representation of the channel. The delay-Doppler-spread function may be considered as a first-order approximation to the rapidly time-varying channel in which each channel component is associated with Doppler shifts that are assumed constant over an averaging interval. The sparse structure of the delay-Doppler-spread function is then exploited by sequentially choosing the dominant components that minimize a least squares error. The advantage of this approach is that it captures both the channel structure as well as its dynamics without the need of explicit dynamic channel modeling. As the symbols are populated with the sample Dopplers, the increase in complexity depends on the channel Doppler spread and can be significant for a severely Doppler-spread channel. Comparison is made between nonsparse recursive least squares (RLS) channel estimation, sparse channel impulse response estimation, and estimation using the proposed approach. The results are demonstrated using experimental data. In training mode, the proposed approach shows a 3-dB reduction in signal prediction error. In decision-directed mode, it improves significantly the robustness of the performance of the channel-estimate-based equalizer against rapid channel fluctuations. 相似文献
239.
The aim of this paper is to investigate the shape and tension distribution of fishing nets in current. A numerical model is developed, based on lumped mass method to simplify the net. The motion equation is set up for each lumped mass. The Runge–Kutta–Verner fifth-order and sixth-order method is used to solve these simultaneous equations, and then the displacement and tension of each lumped mass are obtained. In order to verify the validity of the numerical method, model tests have been carried out. The results by the numerical simulation agree well with the experimental data. 相似文献
240.
Horiguchi T Li Z Uno S Shimizu M Shiraishi H Morita M Thompson JA Levings CD 《Marine environmental research》2004,57(1-2):75-88
Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study. 相似文献