Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)

Cytochrome P450 (CYP) superfamily is one of the membership largest and function most diverse protein superfamily recogniozed among living beings. Members of this superfamily were further assigned to different families and subfamilies based on their amino acid similarities. According to their phylogenetic relationships, the CYP genes which likely diverged from common ancestor gene and may share common functions were grouped into one clan. Widely distributing scallops are a group of the most conspicuous bivalve; however the studies on their CYP is acarce. In this study, we searched the genome and expressed sequence tags of Zhikong scallop (Chlamys farreri) for CYP genes. In total, 88 non-redundant CYP were identified, which were homed in 13 CYPs gene families. Phylogenetic analysis divided these genes into 4 CYP clans. As in deuterostomes, Clan 2 was the largest, which contained 33 genes belonging to CYP1, CYP2, CYP17 and CYP356 families. Clan 3 contgained 19 genes belonging to CYP3, CYP5 and CYP30 families. Clan 4 contained 23 genes, all belonging to CYP4 family. The mitochondrial CYP clan contained 9 genes belonging to CYP10 and CYP24 families. In comparison, protostomes (C. farreri, D. pluex, D. melanogaster) contained more CYP genes than deuterostomes (S. purpuratus and vertebrates) in Clan 2 but less genes in Clan 3 and Clan 4. Our findings will aid to deciphering CYP function and evolution in scallops and bivalves.     

Development of 101 novel EST-derived single nucleotide polymorphism markers for Zhikong scallop (Chlamys farreri)

Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,based on the expressed sequence tags(EST),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled probe.Of them,101(33.7%) were found to be polymorphic in 48 individuals from 4 populations.Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500.The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,respectively.Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers.BLASTx gave significant hits for 72 of the 101 polymorphic SNP-containing ESTs.Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.     

Distribution and controlling factors of phytoplankton assemblages in a semi-enclosed bay during spring and summer

The phytoplankton assemblages' patterns and their correlation to environmental factors were studied in Bohai Bay during spring and summer. Two zones, the northern (NWA) and southern (SWA) water area, were identified by cluster analysis based on their physical and chemical properties. Principal component analysis (PCA) showed that more phytoplankton species was found in the SWA with low nutrient concentration, while high phytoplankton abundance occurred in the NWA with high nutrient concentration. The seasonal variability in phytoplankton can be explained by water temperature, nutrient, and hydrodynamic conditions (includes mixing during spring and stratification during summer). Results of redundancy analysis (RDA) showed that silicate (SiO(4)) and soluble reactive phosphorus (SRP) were the most important environmental factors influencing the phytoplankton distribution during spring and summer, respectively. Hydrodynamics condition plays a key role in controlling variation of the environmental factors, which determined phytoplankton distribution in Bohai Bay.     

1597年10月6日ldquo;珲春-汪清深震区rdquo;Mge;8地震触发的湖震和火山喷发

探讨了1597年10月6日（明万历二十五年八月二十六日）地震的烈度特征,地震伴随的湖震和火山喷发,以及地震类型与规模．结果表明, 1597年10月6日地震为可能发生在ldquo;珲春——汪清深震区rdquo;的一次Mge;8深源地震;地震在中国东部地区产生了广泛的湖震,其多种形态可与1775年葡萄牙里斯本地震产生的湖震比拟;地震触发了望天鹅火山的一次中小规模爆发式喷发,其地点在中国吉林省长白县境内．      

多网重力数据联合平差和系统误差改正

华北地区现行的地震重力监测网由于独立联测, 自成体系, 绝对控制点较少, 获得的重力场动态变化图像在绝对控制较弱的地区会产生畸变, 也不能有效的消除仪器标定系统所引起的测量误差。 本文以华北大网作为控制网, 结合绝对重力点进行联合平差计算, 使各省区地震重力监测网平差精度提高了(0~4)×10^-8 m/s², 平差起算基准相统一, 绝对控制较弱的地区畸变减小。 采用相关系数检验法, 对华北地区各省区地震重力监测网2010年二期重力平差计算结果进行检验, 部分重力点位系统误差高达60×10^-8 m/s², 经过系统误差改正后, 重力场动态变化趋于一致。 经过各项改正后, 华北地区2010年重力场动态差分图像对地震前兆具有更好的反映。     

地震活动性预测并划分未来地震发生的潜在区域方法研究

采用网格地震方法,选取面积百分比(33%),确定圈闭区域的半径,统计后期地震发生在圈闭区域内的概率。在我国4个不同构造环境和地震活动水平的地区,分别统计了后期地震发生在圈闭区域内的概率。结果表明,4个地区后期地震发生在圈闭区域的概率远远大于面积百分比。说明未来地震是高度地聚集在过去地震发生的区域内,采用地震活动性预测并划分未来地震发生的潜在区域的方法是可行的。     

近震信号检测参数设置研究

地震信号检测是进行各种地震数据分析和处理的首要任务,STA/LTA方法具有算法简单、便于实时处理等特点,被广泛应用于地震信号检测.结合实际震例数据研究STA/LTA方法进行地震信号检测的各种影响因素,得到该方法进行检测时最合理的参数设置范围.     

云南水氡群体异常特征

采用非均匀度方法,分别提取滇西北、滇中北、滇西南、滇东南和滇东北地区水氡观测资料的地震前兆群体异常。结果表明,该方法能满足水氡观测点所在地区成组中强地震的中、短临预报要求;且该方法能自动识别异常,排除人为主观影响,便于在其他震区进行预报效能检验。     

中国大陆重力场非潮汐时空变化特征的初步分析

本文基于Tsoft软件在潮汐数据预处理中的应用,采用Tsoft数据预处理软件和别尔采夫滤波相结合方法,提取了覆盖中国大陆28个重力固体潮台站连续观测数据的非潮汐分量.对各台站2008～2011年的连续数据,进行了缺失数据补接、地震动事件提取和去除非线性漂移等预处理工作,并通过滤波提取固体潮非潮汐分量.结果表明:除在贵阳、勐腊和十堰3个原始数据质量较差的台站外,其它台站观测数据应用本文方法均取得良好的结果.在此基础上,对处理结果采用滑动平均,将非潮汐分量结果的月均值与GRACE卫星和CMAP降水记录的月采样数据进行对比,结果显示了地表降水同重力变化具有一定相关性特征.此外,基于Tsoft软件的分段曲线漂移拟合能有效去除原始数据中的漂移.     

Finite element analysis of steep excavation slope failure by CFS theory

The distribution of Coulomb failure stress (CFS) change in the steep excavation slope is calculated by finite element method in this paper, and the failure mechanics under different conditions have been investigated. Comparing the CFSs before and after the slope excavation (stress loading and unloading processes), the dangerous internal zone and the most likely failure external area are attained. Given the shear cracks on the top surface while tensile stress or cracks along the toe of the slope, we analyze the high cutting-angle steep slope in Kaixian county of the Three Gorges Reservoir region. We bring forward that the peak value of CFS after excavation can reach to the order of 0.1 MPa, which is greatly higher than that of before. Our preliminary results are useful for optimizing the reinforcement structure during the steep slope stabilization engineering.