Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study

Pelagic copepods play an important role in the marine food web. However, a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-based copepod diet studies, we found many apostome ciliates that live symbiotically under the exoskeleton of the copepods, and their sequences were often over-represented in the 18S rRNA gene （18S rDNA） libraries. As a first step to address this issue, we designed three apostome ciliate 18S rDNA blocking primers, and tested their blocking efficiency against apostome ciliate 18S rDNA under various PCR conditions. Using a semi-quantitative PCR method, we optimized the conditions to efficiently amplify the 18S rDNA of the prey while simultaneously excluding the symbiotic apostome ciliates. This technique will facilitate PCR-based diet studies of copepods and other zooplankton in their natural environments.     

Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales,Phaeophyta)

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.     

Characteristics of a ridge-transform inside corner intersection and associated mafic-hosted seafloor hydrothermal field (14.0°S,Mid-Atlantic Ridge)

Morphotectonic analysis of the inside corner intersection (14.0°S) between the southern Mid-Atlantic Ridge and the Cardno fracture zone indicate a young rough massif emerging after the termination of a previous oceanic core complex. The massif, which hosts an off-axis hydrothermal field, is characterized by a magmatic inactive volcanic structure, based on geologic mapping and sample studies. Mineralogical analyses show that the prominent hydrothermal deposit was characterized by massive pyrite-marcasite breccias with silica-rich gangue minerals. Geochemical analyses of the sulfide breccias indicate two element groups: the Fe-rich ore mineral group and silica-rich gangue mineral group. Rare earth element distribution patterns showing coexistence of positive Eu anomalies and negative Ce anomalies suggest that sulfides were precipitated from diffused discharge resulted from mixing between seawater and vent fluids. Different from several low temperature hydrothermal systems occurring on other intersection dome-like massifs that are recognized as detachment fault surfaces associated with variably metamorphosed ultramafic rocks, the 14.0°S field, hosted in gabbroic-basaltic substrate, is inferred to be of a high temperature system and likely to be driven by deep high temperature gabbroic intrusions. Additionally, the subsurface fossil detachment fault is also likely to play an important role in focusing hydrothermal fluids.     

白令海和楚科奇海表层沉积物中多环芳烃降解微生物多样性

为了研究白令海(海盆及陆架)至楚科奇海陆架表层沉积物中多环芳烃(PAHs)降解菌的多样性,并获得新的PAHs降解菌资源。在GC-MS分析沉积物中PAHs种类和含量的基础上,以萘、菲和芘的混合物为唯一碳源和能源对表层沉积物样品进行富集,通过平板分离鉴定可培养菌株,并验证其降解能力;同时利用变性梯度凝胶电泳(DGGE)和Illumina高通量测序技术分析降解菌群结构。GC-MS测定结果表明,14个表层沉积物中PAHs总干质量介于32.99~276.97ng/g。富集菌群中共分离获得51株可培养细菌,平板纯培养、PCR-DGGE及Illumina测序结果均表明,菌群中优势的降解菌是γ-proteobacteria的Marinobacter,Pseudoalteromonas,Pseudomonas和Actinobacteria门的Dietzia菌。此外,Illumina测序结果还表明14个降解菌群在菌群结构组成上,可分为海盆区和陆架区两种类群;同时检测到一些低丰度的海洋专属PAHs降解菌,如Cycloclasticus,Alteromonas和Neptunomonas等。本文结果将加深对白令海及楚科奇海表层沉积物中PAHs降解菌资源与生物多样性的认识。     

利用GPS定位检测伞降式火箭下降轨迹初探

文章概要介绍了利用GPS定位测试伞降式火箭残骸下降轨迹的研究进展,通过在伞降式火箭弹内安装GPS定位模块,检测火箭弹开伞后伞降速度、落点的方位仰角,并估算伞降弹道,借此对火箭弹开伞后的安全性进行全方位评估,为最终形成人工影响天气火箭弹动态检测业务运行能力提供基础。     

Effect of sodium chloride and cadmium on the growth,oxidative stress and antioxidant enzyme activities of Zygosaccharomyces rouxii

Zygosaccharomyces rouxii is a salt-tolerant yeast species capable of removing cadmium(Cd) pollutant from aqueous solution. Presently, the physiological characteristics of Z. rouxii under the stress of sodium chloride(NaCl) and Cd are poorly understood. This study investigated the effects of NaCl and Cd on the growth, oxidative stress and antioxidant enzyme activities of Z. rouxii after stress treatment for 24 h. Results showed that NaCl or Cd alone negatively affected the growth of Z. rouxii, but the growth-inhibiting effect of Cd on Z. rouxii was reduced in the presence of NaCl. Flow cytometry assay showed that under Cd stress, NaCl significantly reduced the production of reactive oxygen species(ROS) and cell death of Z. rouxii compared with those in the absence of NaCl. The activities of superoxide dismutase(SOD), catalase(CAT), and peroxidase(POD) of Z. rouxii were significantly enhanced by 2%–6% NaCl, which likely contributed to the high salt tolerance of Z. rouxii. The POD activity was inhibited by 20 mg L-1Cd while the SOD and CAT activities were enhanced by 8 mg L-1 Cd and inhibited by 20 mg L-1 or 50 mg L-1 Cd. The inhibitory effect of high-level Cd on the antioxidant enzyme activities of Z. rouxii was counteracted by the combined use of NaCl, especially at 6%. This probably accounted for the decrease in Cd-induced ROS production and cell death of Z. rouxii after incubation with NaCl and Cd. Our work provided physiological clues as to the use of Z. rouxii as a biosorbent for Cd removal from seawater and liquid highly salty food.     

The effect of environmental factors on spatial variability in land use change in the high-sediment region of China’s Loess Plateau

In areas with topographic heterogeneity, land use change is spatially variable and influenced by climate, soil properties, and topography. To better understand this variability in the high-sediment region of the Loess Plateau in which soil loss is most severe and sediment diameter is larger than in other regions of the plateau, this study builds some indicators to identify the characteristics of land use change and then analyze the spatial variability as it is affected by climate, soil property, and topography. We build two indicators, a land use change intensity index and a vegetation change index, to characterize the intensity of land use change, and the degree of vegetation restoration, respectively. Based on a subsection mean method, the two indicators are then used to assess the spatial variability of land use change affected by climatic, edaphic, and topographic elements. The results indicate that: 1) Land use changed significantly in the period 1998-2010. The total area experiencing land use change was 42,302 km2, accounting for 22.57% of the study area. High-coverage grassland, other woodland, and forest increased significantly, while low-coverage grassland and farmland decreased in 2010 compared with 1998. 2) Land use change occurred primarily west of the Yellow River, between 35 and 38 degrees north latitude. The four transformation types, including (a) low-coverage grassland to medium-coverage grassland, (b) medium-coverage grassland to high-coverage grassland, (c) farmland to other woodland, and (d) farmland to medium-coverage grassland, were the primary types of land use change, together constituting 60% of the area experiencing land use change. 3) The spatial variability of land use change was significantly affected by properties of dryness/wetness, soil conditions and slope gradient. In general, land use changed dramatically in semi-arid regions, remained relatively stable in arid regions, changed significantly in clay-rich soil, remained relatively stable in clay-poor soil, changed dramatically in steeper slopes, and remained relatively stable in tablelands and low-lying regions. The increase in vegetation coincided with increasing changes in land use for each physical element. These findings allow for an evaluation of the effect of the Grain to Green Program, and are applicable to the design of soil and water conservation projects on the Loess Plateau of China.     

北京交通网络拓扑结构及可达性格局历史变化研究

基于过去800年（1160～2000年）北京城市交通网络时间序列数据,研究了北京交通网络的发展变化。主要从交通网络的拓扑结构、可达性等角度分别对网络直径、平均最短路径长度、平均聚集指数、相对可达性等指标进行了定性定量分析。研究结果表明：第一,北京交通网络扩张呈“两段式”特点：前数百年只加密路网,近53年网络直径扩张了2．62倍;第二,从网络拓扑结构的角度分析,北京过去800年交通网络度分布结构呈现良好的一致性,交通网络中顶点与路段的比例保持不变,网络内部联系日趋紧密;第三,北京交通网络可达性格局趋于有序：从金元时期的不规则格局发展为明清时期的椭圆形格局,建国后呈圆盘形格局扩张,但可达性中心由天安门向西北方向偏移至阜成门处。通过搜集研究北京过去800年的交通网络数据,本文记录了北京交通网络演化的全过程,并揭示了其演化过程中蕴含的宏观规律。     

TEQC在GPS数据分析及多路径效应中的应用研究

介绍了TEQC及其辅助软件QCVIEW 32的功能以及在DOS系统下的操作命令,并运用这两种软件对试验数据进行了GPS多路径误差分析,得出了影响多路径误差的部分因素,试验证明,TEQC可以方便地用于多路径误差分析,并能取得良好的效果。     

川西高原红原机场建设气象条件可行性分析

在为川西高原红原机场气候论证观测取得连续一年多资料的基础上,结合相邻的红原县、阿坝县气象站30年整编气候资料,利用山地气候推算分析方法,对机场场址的气候资料进行推算分析,根据推算的气候资料结合实际观测资料,对机场建设可能产生影响的气象要素进行分析评估,形成了可行性综合论证报告。本研究为红原机场工程建设提供了气象科学依据,取得了良好的社会效益和经济效益。