Thermolability of glycolysis enzymes from a psychrophilic marine bacterium

Cell extracts ofVibrio marinus MP-1 grown at 15°C and suspended in either tris-HCl buffer or NaCl were compared for heat lability of hexokinase, glucose phosphate isomerase, and aldolase. In tris buffer glucose phosphate isomerase and aldolase lost 50 per cent activity after one hour at low thermal exposures of 34°C and 28°C, respectively. Complete loss of enzyme activity occurred at 36°C and 34°C, respectively. Hexokinase suspended in tris buffer lost all activity above 17°C. The cell extracts suspended in 3.6 per cent NaCl were more heat stable and the enzymes lost 50 per cent activity after exposure between 31°C and 35°C. The enzymes lost all activity on thermal exposure of 33°C to 38°C in the presence of NaCl.     

Effects of environmental parameters of low temperature and hydrostatic pressure on L-serine deamination byVibrio marinus

Vibrio marinus, an obligately psychrophilic marine bacterium, deaminated nine of 17 amino acids tested with both L-glutamine and L-serine displaying the greatest deamination rates.The L-serine deamination temperature response of washed cells depended upon the growth temperature ofV. marinus MP-1. Cells grown at 15°C displayed optimum activity at 40°C, and a shoulder at 15°C, whereas 4°C grown cells revealed two temperature optima, one at 38 and the other at 11°C, this suggests that the 4°C grown cells are physiologically different than the 15°C grown cells.It is suggested that these peaks in deamination of L-serine at different temperatures might be due to the loss of permeability control above the maximum growth temperature (20°C) of the organism.Hydrostatic pressure stimulated or suppressed L-serine deamination by washed cells depending upon the temperature at which the cells were grown and the incubation temperature of the reaction mixture. Cells grown at 15 or 4°C had deamination stimulated under pressure in the following cases: (i) cells grown at 15°C and tested for deamination at 15°C, (ii) cells grown at 4°C and tested at 4°C and (iii) cells grown at 4°C and tested at 15°C. When cells were grown at 15°C and tested at 4°C no stimulation of deamination activity due to pressure was observed.     

Processes influencing iron distribution in the coastal waters of the Tsugaru Strait,Japan

We report measurements of iron, nutrients, dissolved oxygen, humic-type fluorescence intensity and chlorophyll a concentrations in the coastal waters at the inflow (western) and outflow (eastern) ends of Tsugaru Strait (Japan) in June 2003 and 2004. Two different water masses (intensive eastward flow “subtropical Tsugaru Warm Current Water (TWCw)” and weak westward flow “subarctic Oyashio Water (OW)”) were observed at the eastern end of the strait. TWCw at the southern part of the eastern strait was vertically homogeneous with a uniform concentrations of iron (0.7–1.1 nM for labile dissolved Fe and 14–20 nM for total dissolvable Fe in 2003) as well as other chemical, biological and physical components throughout the water column of 200 m due to strong vertical mixing in the strait. The degree of mixing in the Tsugaru Warm Current (TWC) is predominantly affected by diurnal tidal current, which is strong during the period of tropical tides and weak during the period of equinoctial ones. The especially strong vertical water mixing in 2003 is caused by large dissipation energy input due to the bottom friction of passage-flow through the strait and tidal current. At the northern part of the eastern strait, the fresh surface layer overlying the OW and the deep-bottom waters in 2003 contained large concentrations of dissolved iron, resulting from iron supplied from river runoff and shelf sediments, respectively. These results suggest that the most important mechanism for transporting iron in the strait is the strong vertical water mixing due to the tidal current, and that the iron sources in the coastal waters are the organic-associated, iron-rich freshwater input into the surface water.     

Seismic imaging of the Formosa Ridge cold seep site offshore of southwestern Taiwan

Multi-scale reflection seismic data, from deep-penetration to high-resolution, have been analyzed and integrated with near-surface geophysical and geochemical data to investigate the structures and gas hydrate system of the Formosa Ridge offshore of southwestern Taiwan. In 2007, dense and large chemosynthetic communities were discovered on top of the Formosa Ridge at water depth of 1125 m by the ROV Hyper-Dolphin. A continuous and strong BSR has been observed on seismic profiles from 300 to 500 ms two-way-travel-time below the seafloor of this ridge. Sedimentary strata of the Formosa Ridge are generally flat lying which suggests that this ridge was formed by submarine erosion processes of down-slope canyon development. In addition, some sediment waves and mass wasting features are present on the ridge. Beneath the cold seep site, a vertical blanking zone, or seismic chimney, is clearly observed on seismic profiles, and it is interpreted to be a fluid conduit. A thick low velocity zone beneath BSR suggests the presence of a gas reservoir there. This “gas reservoir” is shallower than the surrounding canyon floors along the ridge; therefore as warm methane-rich fluids inside the ridge migrate upward, sulfate carried by cold sea water can flow into the fluid system from both flanks of the ridge. This process may drive a fluid circulation system and the active cold seep site which emits both hydrogen sulfide and methane to feed the chemosynthetic communities.     

Upward transport of iron at the west shelf edge–slope of the Okinawa Trough in the East China Sea

We studied the behavior of chemical substances in the upper 300 m of the water column across the continental shelf–slope interface in the East China Sea off the Okinawa Trough. The behaviors of iron, inorganic nutrients, and humic-like fluorescent dissolved organic matter were strongly influenced by the extensive water exchange between the East China Sea and the Kuroshio Current across the shelf break and slope via upwelling and frontal processes. We attributed the high humic-like fluorescent intensity at the subsurface of the shelf break and slope regions to the lateral supply of humic-like fluorescent dissolved organic matter from the shelf sediments to the outer shelf region due to the intrusion of shelf water into Kuroshio subsurface water. We found that the behavior of iron at the continental shelf–slope was remarkably different from the conservative mixing of inorganic nutrients and humic-like fluorescent dissolved organic matter. In deep and bottom waters at the shelf–slope, high total iron concentrations, which were closely related to water transmittance, possibly resulted from the swept transport of iron-rich resuspended sediments over the shelf floor from the slope by the invading Kuroshio Intermediate Water close to the bottom.     

A New Solar Imaging System for Observing High-Speed Eruptions: <Emphasis Type="Italic">Solar Dynamics Doppler Imager</Emphasis> (SDDI)

A new solar imaging system was installed at Hida Observatory to observe the dynamics of flares and filament eruptions. The system (Solar Dynamics Doppler Imager; SDDI) takes full-disk solar images with a field of view of \(2520~\mbox{arcsec} \times 2520~\mbox{arcsec}\) at multiple wavelengths around the \(\mathrm{H}\alpha\) line at 6562 Å. Regular operation was started in May 2016, in which images at 73 wavelength positions spanning from \(\mathrm{H}\alpha -9~\mathring{\mathrm{A}}\) to \(\mathrm{H}\alpha +9~\mathring{\mathrm{A}}\) are obtained every 15 seconds. The large dynamic range of the line-of-sight velocity measurements (\({\pm}\,400~\mbox{km}\,\mbox{s}^{-1}\)) allows us to determine the real motions of erupting filaments in 3D space. It is expected that SDDI provides unprecedented datasets to study the relation between the kinematics of filament eruptions and coronal mass ejections (CME), and to contribute to the real-time prediction of the occurrence of CMEs that cause a significant impact on the space environment of the Earth.     

Variation of dissolved organic matter and fluorescence characteristics before,during and after phytoplankton bloom

The variation of dissolved organic matter (DOM) and fluorescence characteristics during the phytoplankton bloom were investigated in Yashima Bay, at the eastern part of the Seto Inland Sea, Japan. We found significant accumulations of dissolved organic carbon (DOC), dissolved organic nitrogen (DON), chromophoric dissolved organic matter (CDOM) fluorescence, and UV₂₆₀ during the phytoplankton bloom period in 2005, although lower accumulations of DOC and DON and only increases of CDOM fluorescence were observed during the bloom period in 2006. Little or no correlation between DOM and phytoplankton abundance might be due to the composition of DOM, which is a complex mixture of organic materials. The 3D-EEM results revealed that the DOM produced around the phytoplankton bloom period contained tyrosine, tryptophan, and humic-like substances. Our results showed that the occurrence of phytoplankton bloom contributed to the production of DOM in coastal water but the DOM accumulation depended on the type of phytoplankton bloom, the phytoplankton species in particular. From our results, we concluded that phytoplankton have a great role in the dynamics of DOM as a producer in a coastal environment.