Biocalcification in the Eastern Oyster (<Emphasis Type="Italic">Crassostrea virginica)</Emphasis> in Relation to Long-term Trends in Chesapeake Bay pH

Anthropogenic carbon dioxide (CO₂) emissions reduce pH of marine waters due to the absorption of atmospheric CO₂ and formation of carbonic acid. Estuarine waters are more susceptible to acidification because they are subject to multiple acid sources and are less buffered than marine waters. Consequently, estuarine shell forming species may experience acidification sooner than marine species although the tolerance of estuarine calcifiers to pH changes is poorly understood. We analyzed 23 years of Chesapeake Bay water quality monitoring data and found that daytime average pH significantly decreased across polyhaline waters although pH has not significantly changed across mesohaline waters. In some tributaries that once supported large oyster populations, pH is increasing. Current average conditions within some tributaries however correspond to values that we found in laboratory studies to reduce oyster biocalcification rates or resulted in net shell dissolution. Calcification rates of juvenile eastern oysters, Crassostrea virginica, were measured in laboratory studies in a three-way factorial design with 3 pH levels, two salinities, and two temperatures. Biocalcification declined significantly with a reduction of ∼0.5 pH units and higher temperature and salinity mitigated the decrease in biocalcification.     

Assessing landslide movements in volcanic islands using near-shore marine geophysical data: south Pico island,Azores

Marine geophysical data from around the submarine flanks of volcanic islands can potentially help to resolve whether large-scale instability of an edifice has been geologically recently active. We use geophysical data to investigate part of the coast of Pico Island of the Azores where, above sea-level, a major slump of Topo volcano has been interpreted previously from arcuate escarpments and a rugged irregular topography seaward of them. Multibeam echo-sounder data collected offshore of this feature show remarkably little evidence for slump fault movements in the island's submarine slope. Mid-slope benches, like those associated with the Hilina Slump of Kilauea, are absent. The high-resolution data extends onto the island's shelf, allowing us to evaluate evidence for continuing activity there. In particular, as the shelf's rock platform will have been last modified by surf erosion during the postglacial period of sea-level transgression, it provides a reference surface of intermediate age (7–19 ka) that can potentially reveal whether any movements occurred in pre-historic times. Where the arcuate escarpments are continued offshore, the modern seabed shows no bathymetric evidence for active faults where the shelf rock platform crops out in the multibeam data. Elsewhere, mobile shelf sediments could be disguising evidence for active faulting so we examined boomer profiles able to image the rock platform beneath them. The data reveal a platform that is steep (6.6°) compared with the dips of platforms that we have studied previously around the coast of adjacent Faial Island and steeper than the platform outside the proposed slump. This suggests that it was created by coastal erosion over a shorter period and hence is consistent with a younger age of the coastline. As with the multibeam data, where escarpments are continued offshore onto the shelf, the rock surface imaged with these boomer data also shows no clear evidence of major slump-related fault displacements. This study therefore illustrates how high-resolution boomer seismic and multibeam data could usefully contribute to hazard assessment of volcanic islands, by helping to evaluate areas with no historical movements. Explanations to reconcile the onshore and offshore data here are also put forward.     

A multi-equation spatial econometric model,with application to EU manufacturing productivity growth

A multi-equation spatial econometric model is used to explain variations across EU regions in manufacturing productivity growth based on recent theoretical developments in urban economics and economic geography. The paper shows that temporal and spatial parameter homogeneity is an unrealistic assumption, contrary to what is typically assumed in the literature. Constraints are imposed on parameters across time periods and between core and peripheral regions of the EU, with the significant loss of fit providing overwhelming evidence of parameter heterogeneity, although the final model does highlight increasing returns to scale, which is a central feature of contemporary theory.      

Discrete numerical modeling of granular materials considering crushability

The aim of this study is to numerically investigate the influence of particle breakage on the mechanical behavior of granular materials using a discrete element method (DEM). To enable particle crushing, non-crushable elementary particles are boned together to represents the granular aggregates which can be crushed when the external force exceeds its strength. The flaw of the aggregate was also modeled by randomly distributed void. Single particle crushing tests were carried out to determine the distribution of particle strength. The results of single particle crushing tests illustrate that the simulated single particle fracture strength and pattern agree well with the Weibull’s distribution equation. Conventional oedometer tests, drained monotonic and cyclic triaxial tests were also carried out to investigate the crushing of the aggregates and the associated mechanical behaviors. The effect of confining pressure on the crushing of aggregates and the mechanical behavior was also analyzed. It was found that the peak stress and dilation decrease significantly when particle crushing was considered. The deformation behavior of the specimen is essentially controlled by two factors: particle rearrangement - induced dilation and particle crushing - induced contraction. The increase of permanent strain and the reduction of dilation were observed during cyclic loading and they tend to reach a stable state after a certain number of cycles. The crushing of aggregate is most significant in the first two cycles. The results also indicated that for the same axial strain the volumetric strain and the bound breakage in the cyclic loading tests are significantly larger than that in the monotonic loading tests, especially at high cyclic stress ratio.     

Selecting suitable sites for mountain ginseng (<Emphasis Type="Italic">Panax ginseng</Emphasis>) cultivation by using geographically weighted logistic regression

With the well-being trends to pursue a healthy life, mountain ginseng (Panax ginseng) is rising as one of the most profitable forest products in South Korea. This study was aimed at evaluating a new methodology for identifying suitable sites for mountain ginseng cultivation in the country. Forest vegetation data were collected from 46 sites and the spatial distribution of all sites was analyzed using GIS data for topographic position, landform, solar radiation, and topographic wetness. The physical and chemical properties of the soil samples, including moisture content, pH, organic matter, total nitrogen, exchangeable cations, available phosphorous, and soil texture, were analyzed. The cultivation suitability at each site was assessed based on the environmental conditions using logistic regression (LR) and geographically weighted logistic regression (GWLR) and the results of both methods were compared. The results show that the areas with northern aspect and higher levels of solar radiation, moisture content, total nitrogen, and sand ratio are more likely to be identified as suitable sites for ginseng cultivation. In contrast to the LR, the spatial modeling with the GWLR results in an increase in the model fitness and indicates that a significant portion of spatial autocorrelation in the data decreases. A higher value of the area under the receiver operating characteristic (ROC) curve presents a better prediction accuracy of site suitability by the GWLR. The geographically weighted coefficient estimates of the model are non-stationary, and reveal that different site suitability is associated with the geographical location of the forest stands. The GWLR increases the accuracy of selecting suitable sites by considering the geographical variations in the characteristics of the cultivation sites.     

Peat humification- and δ<Superscript>13</Superscript>C<Subscript>cellulose</Subscript>-recorded warm-season moisture variations during the past 500 years in the southern Altai Mountains within northern Xinjiang of China

To predict future spatio-temporal patterns of climate change, we should fully understand the spatio-temporal patterns of climate change during the past millennium. But, we are not yet able to delineate the patterns because the qualities of the retrieved proxy records and the spatial coverage of those records are not adequate. Northern Xinjiang of China is one of such areas where the records are not adequate. Here, we present a 500-yr land-surface moisture sequence from Heiyangpo Peat (48.34°N, 87.18°E, 1353 m a.s.l) in the southern Altai Mountains within northern Xinjiang. Specifically, peat carbon isotope value of cellulose (δ¹³C_cellulose) was used to estimate the warm-season moisture variations and the degree of humification was used to constrain the δ¹³C_cellulose-based hydrological interpretation. The climatic attributions of the interpreted hydrological variations were based on the warm-season temperature reconstructed from Belukha ice core and the warm-season precipitation inferred from the reconstructed Atlantic Multidecadal Oscillations (AMO). The results show that humification decreased and the δ¹³C_cellulose-suggested moisture decreased from ~1510 to ~1775 AD, implying that a constant drying condition may have inhibited peat decay. Our comparison with reconstructed climatic parameters suggests that the moisture-level decline was most likely resulted from a constant decline of precipitation. The results also show that humification kept a stable level and the δ¹³C_cellulose-suggested moisture also decreased from ~1775 to ~2013 AD, implying that peat decay in the acrotelm primarily did not depend on the water availability or an aerobic environment. Again, our comparison with reconstructed climatic parameters suggests that the land-surface moisturelevel decline was most likely resulted from a steady warming of growing-season temperature.     

Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

Identification of Gleissberg Cycles and a Rising Trend in a 315-Year-Long Series of Sunspot Numbers

We show in this short note that the method of singular spectrum analysis (SSA) is able to clearly extract a strong, clean, and clear component from the longest available sunspot (International Sunspot Number, ISN) time series (1700?–?2015) that cannot be an artifact of the method and that can be safely identified as the Gleissberg cycle. This is not a small component, as it accounts for 13% of the total variance of the total original signal. Almost three and a half clear Gleissberg cycles are identified in the sunspot number series. Four extended solar minima (XSM) are determined by SSA, the latest around 2000 (Cycle 23/24 minimum). Several authors have argued in favor of a double-peaked structure for the Gleissberg cycle, with one peak between 55 and 59 years and another between 88 and 97 years. We find no evidence of the former: solar activity contains an important component that has undergone clear oscillations of \(\approx90\) years over the past three centuries, with some small but systematic longer-term evolution of “instantaneous” period and amplitude. Half of the variance of solar activity on these time scales can be satisfactorily reproduced as the sum of a monotonous multi-secular increase, a \(\approx90\)-year Gleissberg cycle, and a double-peaked (\(\approx10.0\) and 11.0 years) Schwabe cycle (the sum amounts to 46% of the total variance of the signal). The Gleissberg-cycle component definitely needs to be addressed when attempting to build dynamo models of solar activity. The first SSA component offers evidence of an increasing long-term trend in sunspot numbers, which is compatible with the existence of the modern grand maximum.     

Variations in the Solar Coronal Rotation with Altitude – Revisited

Here we report an in-depth reanalysis of an article by Vats et al. (Astrophys. J. 548, L87, 2001) that was based on measurements of differential rotation with altitude as a function of observing frequencies (as lower and higher frequencies indicate higher and lower heights, respectively) in the solar corona. The radial differential rotation of the solar corona is estimated from daily measurements of the disc-integrated solar radio flux at 11 frequencies: 275, 405, 670, 810, 925, 1080, 1215, 1350, 1620, 1755, and 2800 MHz. We use the same data as were used in Vats et al. (2001), but instead of the twelfth maxima of autocorrelograms used there, we use the first secondary maximum to derive the synodic rotation period. We estimate synodic rotation by Gaussian fit of the first secondary maximum. Vats et al. (2001) reported that the sidereal rotation period increases with increasing frequency. The variation found by them was from 23.6 to 24.15 days in this frequency range, with a difference of only 0.55 days. The present study finds that the sidereal rotation period increases with decreasing frequency. The variation range is from 24.4 to 22.5 days, and the difference is about three times larger (1.9 days). However, both studies give a similar rotation period at 925 MHz. In Vats et al. (2001) the Pearson’s factor with trend line was 0.86, whereas present analysis obtained a \({\sim}\,0.97\) Pearson’s factor with the trend line. Our study shows that the solar corona rotates more slowly at higher altitudes, which contradicts the findings reported in Vats et al. (2001).     

Reconstruction of the North–South Solar Asymmetry with a Kuramoto Model

This paper applies a Kuramoto model of coupled oscillators to investigate the north–south (N–S) solar asymmetry and properties of meridional circulation. We focus our study on the asymmetry of the 11-year phase, which is slight but persistent: only two changes of sign (around 1928 and 1968) are observed in the past century. We present a model of two non-linear coupled oscillators that links the hemispheric phase asymmetry of sunspots with the asymmetry of the meridional flow. We use a Kuramoto model with evolving frequencies and constant symmetric coupling to show how asymmetry in meridional circulation could produce a persistent phase lead of one solar hemisphere over the other. We associate the natural frequencies of the two oscillators with the velocities of the meridional flow cells in the northern and southern hemispheres. We assume the respective circulations to be independent and estimate the value of the relevant cross-equatorial coupling by the coupling coefficient in the Kuramoto model. We find that a persistent N–S asymmetry of sunspots and the change of the leading hemisphere could indeed both be the result of the evolving frequencies of meridional circulation; the necessary asymmetry of the meridional flow may be small; and the cross-equatorial coupling has an intermediate range value. Possible applications of these results in solar dynamo models are discussed.