Sediment Transport in Rivers and Coastal Waters

Following Bagnold's approach, a relationship between sediment transport and energy dissipation is developed. The major assumption made in the study is that the near bed velocity plays a dominant role in the process of sediment transport. A general relationship between energy dissipation and sediment transport is first proposed. Then the equations for total sediment transport are derived by introducing the appropriate expression of energy dissipation rate under different conditions, such as open channel flows, combination of wave and current, as well as longshore sediment transport. Within the flows investigated, the derived relationships are fairly consistent with the available data over a wide range of conditions.     

Hydroelastic Analysis of Very Large Floating Structures Using Plate Green Functions

1 .IntroductionRecentlygreatinteresthasbeenshowninthedevelopmentofverylargefloatingstructuressuchasMegaFloatofJapan (Isobe ,1 999)andMOBofUSA (Remmers ,1 999) .Owingtotheirextremelargesizeandgreatflexibility ,thecouplingbetweenthestructuraldeformationandfluidmotionissignifi cant.Thisisatypicalproblemofhydroelasticity .Efficientandaccurateestimationofthehydroelasticresponseofverylargefloatingstructuresinwavesisveryimportantfordesign .Manymethodshavebeenproposedinliteratureforthepredictiono…     

浮游植物叶绿素a含量简易测定方法的比较

用乙醇、DMF(N,N-二甲基甲酰胺)、丙酮3种不同有机溶剂分别萃取苏州环城河水样中的浮游植物细胞叶绿素a,在分光光度计上测定其叶绿素a含量,试验数据经方差分析、Q检验,结果显示,乙醇法、DMF法的叶绿素a萃取率极显著高于丙酮(F=54.20＞F0.01(2,18)(6.01)).另用这3种萃取方法分别测定无常蓝纤维藻(Dactylococcopsis irregularis )纯培养液叶绿素a含量,通过直线回归,同样证明乙醇法、DMF法重复率较高,稳定性好,萃取率高.从安全、简便、重复性高等方面综合考虑,认为用乙醇萃取法是目前水域环境中浮游植物叶绿素a简易测定的最佳方法.     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

中国海洋天然活性物质开发新技术21世纪发展战略

海洋天然活性物质是指海水、沉积物和在海洋环境生存的动植物体中所含有的具有生理活性的物质，一般情况下则指海洋生物体中的生物活性物质。几千年来，人们一直在探索和发现这类物质，并扩大它的应用，在当今人类回归大自然的浪潮中，海洋天然活性物质的开发成了国际热点之一 [1～ 9]。本文在分析了国外的进展，我国的水平与差距后，提出了我国海洋天然活性物质开发新技术的发展战略。     

The Working Mechanism and An Analytic Method of the Deep Embedded Large Cylinder Structure

The large cylinder is a new-type structure that has been applied to harbor and offshore engineering. An analytic method of the relationship between loads and the structure displacement is developed based on the failure mode of deep embedded large cylinder structures. It can be used to calculate directly the soil resistance and the ultirnate bearing capacity of the structure under usage. A new criterion of the large cylinder structure, which discriminates the deep embedded cylinder from the shallow embedded cylinder, is defined. Model tests prove that the proposed method is feasible for the analysis of deep embedded large cylinder structures.     

卤虫无节幼体的营养强化对黑斑口虾蛄(Oratosquilla kempi)幼体发育的影响

用扁藻、酵母、“鱼油 酵母”强化和“不强化”4种方式处理的卤虫无节幼体投喂黑斑口虾蛄各期幼体,比较不同强化方法对黑斑口虾蛄幼体成活率、变态率、生长速度及总脂与脂肪酸组成的影响。结果表明,3种方式强化12h后的卤虫无节幼体与对照组相比,总脂含量都有不同程度的增加。卤虫无节幼体的脂肪酸组成与强化方式密切相关,其中“鱼油 酵母”强化的卤虫无节幼体20∶5n-3(EPA)和22∶6n-3(DHA)占总脂肪酸的比例最高,分别为5·74%和4·84%。投喂强化后富含EPA和DHA的卤虫无节幼体,可增加黑斑口虾蛄幼体体内脂肪酸尤其是EPA和DHA等不饱和脂肪酸的含量,从而提高其幼体的成活率、变态率与发育速度。     

Rain-Flow and Reverse Rain-Flow Counting Method for the Compilation of Fatigue Load Spectrum

The rain-flow counting method is widely used to compile the fatigue load spectrum. The second stage counting of the rain-flow method is a troublesome process. In order to overcome this drawback, the rain-flow and reverse rain-flow counting method is proposed in this paper. In this counting method, the rule for counting of the rain-flow method is modified, so that the sequence of load-time need not be adjusted. This is a valid and useful method to count cycles and compile the load spectrum and it can be widely used in ocean engineering.