Chitosan extends the shelf-life of filleted tilapia (Oreochromis niloticus) during refrigerated storage

Shelf-life extension of aquatic products is of significant economical importance. To determine the potential effect of chitosan on the shelf-life of filleted tilapia, this study analyzed the bacterial community diversity in fresh and spoiled tilapia fillets stored at (4 ± 1)℃ and examined the antimicrobial activity of chitosan against relevant bacteria isolates. Results showed that Pseudomonas (20%) and Aeromonas (16%) were abundant in fresh tilapia fillets, whereas Pseudomonas (52%), Aeromonas (32%) and Staphylococcus (12%) were dominant in the spoiled samples. Chitosan showed wide-spectrum antibacterial activity against bacteria isolated from tilapia and 5.0 g L-1 chitosan was selected for application in preservation. We further determined the shelf-life of chitosan-treated, filleted tilapia stored at (4 ± 1)℃ based on microbiological, biochemical and sensory analyses. Results showed that the shelf-life of chitosan-treated, filleted tilapia was extended to 12 d, whereas that of untreated, control samples was 6 d. These indicate that chitosan, as a natural preservative, has great application potential in the shelf-life extension of tilapia fillets.     

塔里木南缘布雅花岗岩和暗色包体的锆石U-Pb年代学及地球化学特征

塔里木西南缘铁克里克地区广泛发育早古生代中酸性侵入岩,本文对其中布雅岩体及其暗色包体进行系统的岩石学、年代学及岩石地球化学研究,确定了岩石成因及其构造属性。LA-MC-ICP-MS锆石U-Pb年代学研究表明,寄主石英二长闪长岩结晶年龄为432.6±2.5 Ma(MSWD=1.5),暗色包体结晶年龄为432.4±6.4 Ma(MSWD=0.031),二者形成时代相同,均为志留纪早期岩浆活动的产物。地球化学特征表明,布雅暗色包体应来源于地幔的部分熔融,而寄主岩石岩浆具有壳源岩浆的性质并经历了幔源岩浆不均匀的混合。野外及岩相学特征均显示暗色包体为铁镁质岩浆注入长英质岩浆快速冷凝形成的,是幔源岩浆底侵下地壳形成的岩浆混合作用的产物。它们是塔里木南缘早古生代碰撞造山作用晚期的岩浆记录。     

西昆仑奇台达坂一带晚三叠世中酸性侵入岩锆石LA-ICP-MS年龄、地球化学特征及其构造意义

奇台达坂位于新疆西昆仑新藏公路540km处,在其周围分布有小规模的中酸性侵入岩,岩体严格受郭扎错—金沙江缝合带控制呈北西向展布,岩性主要为英云闪长岩、石英闪长岩和花岗闪长岩。锆石环带结构明显,Th/U值介于0.15~0.73之间,为典型的岩浆锆石。LA-ICP-MS锆石U-Pb同位素测年结果表明,其形成年龄分别为214.7±2.0 Ma、211.3±3.3 Ma和210.6±2.8 Ma,时代为晚三叠世。主微量元素英云闪长岩→石英闪长岩→花岗闪长岩,岩石中SiO_2(58.96%~67.52%)含量逐渐增高,Al_2O_3(14.79%~16.37%)、CaO(3.41%~5.25%)和Mg O(1.47%~3.42%)逐渐降低;里特曼指数1.03~1.79,K_2O+Na_2O全碱含量为4.36%~6.4%,属钙碱性系列。稀土元素中等(∑REE=101.27×10-6~271.04×10-6),轻重稀土分异明显(LaN/YbN平均为10.69),Eu异常明显(δEu平均值0.60)。大离子亲石元素(LILE)Rb、K、Th富集、相对亏损Ba,高场强元素(HFSE)Nb、Zr、Hf、Sr、P、Ti亏损。对研究区侵入岩进行投图分析,认为它是形成于大陆边缘弧环境的未分异I型花岗岩,物质来源主要为岩浆弧地壳的角闪岩相变玄武岩,并卷入少量的地幔物质。根据岩体的成因类型并结合区域构造环境演化认为,在晚三叠世末,古特提斯洋南缘局限洋盆(巴颜喀拉中生代边缘裂陷海洋盆)发生自NE向SW的持续俯冲消减,洋—陆转换开始,该套中酸性侵入岩侵入就位。     

冻融循环条件下生境基材中三种功能微生物数量变化规律研究

为揭示高寒地区典型生境基材中功能微生物数量变化的规律,以黄棕壤土为试验土样,在两种初始含水率条件下,分别制备客土、植被混凝土以及加菌植被混凝土三种不同类型的生境基材进行冻融循环模拟试验,探讨生境基材类型、初始含水率和冻融循环次数对基材中功能微生物数量的影响。试验结果显示：三种基材中功能微生物数量均受冻融循环影响显著,且随冻融循环次数增加总体呈"下降~上升~下降"趋势;冻融循环过程中,三种基材中功能微生物总量存在显著性差异,排序为：加菌植被混凝土 > 植被混凝土 > 客土,且固氮、解钾微生物数量显著低于解磷微生物;含水率为22%的基材中功能微生物总量普遍高于含水率为18%的基材。研究结果表明,加菌植被混凝土的肥力、抗冻性相比客土和植被混凝土有一定程度提升,但仍需对现有功能菌剂配方加以改进,以适应高寒地区冻融环境。     

Isolation and characterization of melanin pigment from yesso scallop <Emphasis Type="Italic">Patinopecten yessoensis</Emphasis>

Melanin is one of the essential compounds in the pigments of molluscan shells. However, the effects of melanin on color variations in molluscs are largely unknown. Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell. We therefore isolated melanin from the pigmented shells using hydrochloric acid method, and characterized the types of melanin pigments by spectrophotometry. The purified melanin, which was verified by spectrophotometry scanning and HPLC analysis, showed the typical characteristics of melanin absorption spectra and HPLC chromatograms. The contents of pheomelanin and eumelanin in pigmented shells, which were determined by the linear standard curve of melanin at 405 nm and 350 nm absorbance, were 48.23 ± 1.350 and 157.65 ± 5.905 mg, respectively. The present results indicate that the brown-pigmented shells of scallops comprise approximately 76.6% of eumelanin and 23.4% of pheomelanin, which supports the presence of eumelanin-rich pigment in scallop shells. Therefore, the combination of hydrochloric acid extraction and spectrophotometric quantification is a rapid and efficient method to isolate and quantify melanin in shells. This will facilitate the melanin studies related to shell color polymorphism and the selective breeding of bivalves with different shell colors.     

The Effects of Rapid Temperature Changes on HK,PK and HSP70 of Litopenaeus vannamei in Different Seasons

Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subjected to a quick temperature change from 27℃ to 17℃ for the summer case(Cold temperature treatment),or from 17℃ to 27℃ for the winter case(Warm temperature treatment).After 0.5,1,3,6,12,24,48,and 72 h of exposure time,shrimps were sampled and prepared for further analysis.The results showed that the effect of acute temperature changes on activities of HK was significant.Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change.The HSP70 level increased in both cold and warm temperature treatments,suggesting that the rapid temperature changes activated the process of body's self-protection.But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L.vannamei.     

复杂矿区三维地质可视化及数值模型构建

复杂三维地质可视化和数值模型的构建是采矿、岩土及水利水电工程中常常遇到的关键问题。针对数值模拟软件在复杂矿区地质体模型构建及单元网格划分等前处理问题上存在的不直观、工作量大等缺点,结合矿业软件在复杂三维地质体建模的强大优势,在研究线框模型和块体模型差异的基础上,提出了基于线框模型的复杂矿区三维地质可视化及数值模型构建技术。根据线框模型特点不同,以地表和采空区模型为型,分别阐述了基于Surpac的表面模型和实体模型无缝转换成MIDAS/GTS数值模型的有效方法,并将该技术成功应用于冬瓜山铜矿复杂采空区群围岩稳定性研究中。结果表明,该方法是可行、有效的,从而为矿山开采设计、安全分析及复杂地质体结构的岩土工程数值模拟分析提供了一种新的建模方法。     

岩体破裂尺度与频率特征关系及其工程实证研究

将S变换时频分析技术引入微震信号波形分析领域,通过震源破裂尺度,研究微震信号频率特征与岩体变形破坏之间的联系。以西南地区3个水电工程作为研究对象,将大量微震监测数据作为统计样本以减小外部因素对参数计算的影响。在该基础上统计、分析岩体破裂尺度与微震信号频率之间的关系,结合大渡河猴子岩水电站地下厂房微震与多点位移计监测资料进行工程实证研究。研究结果表明,当岩体破裂尺度增大时微震信号高频成分减少,表现出低频特征;在围岩变形过程中微震信号高频成分呈现出先减少再增加的演化规律,围岩发生明显变形前微震信号出现由高频向低频转移的现象,因此,可将聚集区域内微震信号频率降低作为该区域岩体宏观变形破坏的前兆信息。研究结果可为基于微震监测的岩体工程稳定性分析及其灾害预测预报提供参考。     

北山花岗岩深部节理间距分布多重分形研究

岩体节理几何特征定量描述是岩体力学性质和渗透性研究的基础,更是核废料处置库场址筛选和评价过程的主要内容。基于甘肃北山高放废物深地质处置库预选区BS17、BS18和BS19钻孔岩芯节理数量修正数据,针对节理间距建立了类负指数分布数学统计模型,进一步应用于RQD体系定量评价深部岩体完整性,通过与RID体系对比验证了概率模型的可靠性。指出节理数量-间距关系具有明显的分段线性特征,分维数随着间距增大而增大。确定了节理间距-数量关系符合尺度律,得出了数量与间距关系的多重分形特征。进一步分析了数量与埋深的多重分形特征,初步实现了岩芯节理的定量描述。验证了多重分形谱可以很好地统计分形维特征,尤其是节理几何形状及分布特征。该研究结果可为实现基于钻孔岩芯节理几何特征数据的深部岩体节理三维网格模拟提供参考。     

内蒙古临河区地下水补径排特征及动态变化规律

查明地下水的补给、径流、排泄特征及动态变化规律,可为地下水资源开发与保护提供科学依据。通过长期观测内蒙古临河区地下水变化,综合研究前人资料,分析该区地下水补给、径流和排泄特征及动态变化规律。该区属于河套灌区,人类灌溉活动对地下水的补给占全部补给量的65.5%; 人工开采及排干沟排泄等人为因素排泄占排泄总量的67.7%。通过分析该区年内地下水动态变化趋势,认为该区灌溉区地下水位动态与灌溉活动一致,城区地下水位动态主要受人工开采活动影响。