Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

台风作用下的港湾型潮滩沉积过程 以2008年“凤凰”台风对福建省罗源湾的影响为例

通过对"凤凰"台风的现场观测和沉积物样品的分析,结果表明,在台风影响下潮水淹没时间增长,增水达1.1 m;台风期间互花米草盐沼内流速变化较复杂,而且盐沼内部流速大于盐沼边缘的;台风期间盐沼边缘潮周期平均悬沙含量是台风前2 d的7倍;台风期间十分之一波高最大为1.54 m。滩面重复测量结果显示,台风登陆期间整个光滩滩面都发生了侵蚀,盐沼内部有部分地区发生侵蚀,侵蚀深度为4.5~5.5 cm,盐沼边缘的侵蚀深度仅为0.7 cm,侵蚀原因主要是植被在风浪作用下从根部折断,从而带走了滩面的沉积物;随着台风强度的减弱,整个滩面均接受悬沙沉降;台风带来的强降雨是影响滩面沉积物活动的重要因素。     

东海陆架两期沙脊的时空对比东海陆架两期沙脊的时空对比

基于高分辨率的单道地震和多波束测深数据,识别并对比了东海陆架中部同一海区相距20余万年的层U14和层U2两期沙脊群,其中层U14期沙脊属于埋藏沙脊,位于东海海底以下90 m深处,推测属于距今320~200 ka的海侵体系域(TST),沙脊顶界面是该期海侵的最大洪泛面(MFS);层U2期沙脊位于东海陆架,属于衰退沙脊,系末次盛冰期(LGM)以来的TST,顶界面是LGM以来的MFS。尽管两期沙脊形成年代相距20余万年,地层层位相距近90 m,但是沙脊群总体走向一致,表明距今2×105 a以来东海陆架潮波基本格局稳定。从层U2期可识别出4个亚期沙脊,通过多波束海底地形图可识别出4组走向的沙脊,多亚期、多走向沙脊是LGM以来海平面阶梯状波动在海底地形演变过程中的响应证据。     

贻贝抗菌肽的研究进展

抗菌肽是一类广泛存在于整个生物界中的双亲性小分子碱性多肽,是生物体固有免疫的关键因子之一.自Steiner等在天蚕(Hyaophora cecropia)中发现世界上第一个抗菌肽-天蚕素(Cecropin)后,到目前为止,在各种生物中总共发现了1 200多种抗菌肽.     

Distribution of the nonlinear random ocean wave period

Because of the intrinsic difficulty in determining distributions for wave periods, previous studies on wave period distribution models have not taken nonlinearity into account and have not performed well in terms of describing and statistically analyzing the probability density distribution of ocean waves. In this study, a statistical model of random waves is developed using Stokes wave theory of water wave dynamics. In addition, a new nonlinear probability distribution function for the wave period is presented with the parameters of spectral density width and nonlinear wave steepness, which is more reasonable as a physical mechanism. The magnitude of wave steepness determines the intensity of the nonlinear effect, while the spectral width only changes the energy distribution. The wave steepness is found to be an important parameter in terms of not only dynamics but also statistics. The value of wave steepness reflects the degree that the wave period distribution skews from the Cauchy distribution, and it also describes the variation in the distribution function, which resembles that of the wave surface elevation distribution and wave height distribution. We found that the distribution curves skew leftward and upward as the wave steepness increases. The wave period observations for the SZFII-1 buoy, made off the coast of Weihai (37°27.6′ N, 122°15.1′ E), China, are used to verify the new distribution. The coefficient of the correlation between the new distribution and the buoy data at different spectral widths (ν=0.3−0.5) is within the range of 0.968 6 to 0.991 7. In addition, the Longuet-Higgins (1975) and Sun (1988) distributions and the new distribution presented in this work are compared. The validations and comparisons indicate that the new nonlinear probability density distribution fits the buoy measurements better than the Longuet-Higgins and Sun distributions do. We believe that adoption of the new wave period distribution would improve traditional statistical wave theory.     

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发射光谱法测定化探样品中的Cu、Pb、Zn、Co、Ag、Sn、Mo和B

以Al2O3、K2S2O7、NaF、C作为缓冲剂与样品混匀,装入石墨电极中,以平头石墨电极为上电极,样品电极为下电极进行摄谱,由光电译谱仪测定谱线的黑度值,以锗为内标用内标法测定样品中Cu、Pb、Zn、Co、Ag、Sn、Mo和B的质量分数。     

鄂尔多斯盆地西部上二叠统石千峰组沉积环境地球化学表征

在沉积学、生物和地球物理测井相标志研究基础上,运用锶钡质量分数比值和锶钡散点图法,锶钙和钍铀比值法、沉积磷酸盐法、碳氧同位素和氧化-还原电位法等地球化学方法,根据微量元素和稳定同位素的特征对鄂尔多斯盆地西部上二叠统石千峰组沉积时的古盐度和氧化还原条件进行了详细分析.结果表明:石千峰组为淡水沉积,属干旱、炎热、氧化的陆相湖泊环境.其结论与石千峰组的棕红、紫红和紫灰色陆源碎屑岩沉积特别与红色沉积层为特征是相一致的.     

基于条件平差的卫星双向时间传递链路性能优化方法

卫星双向时间传递(Two-Way Satellite Time and Frequency Transfer, TWSTFT)是目前精度最高的时间传递方法之一,同时也是参与国际原子时计算的守时实验室之间比较原子时尺度的一种主要方法.提高TWSTFT链路的短期稳定度,降低周日效应对链路时间传递结果的影响,对优化TAI (International Atomic Time)的性能具有现实意义.提出了一种基于条件平差的TWSTFT链路性能优化方法,先依据TWSTFT链路测量噪声水平与谱分析结果建立TWSTFT链路性能优化网络(简称优化网络),再根据优化网络中各链路测量噪声分析结果设置权系数阵,建立条件平差模型.选取亚太地区的中国计量科学研究院(National Institute of Metrology, NIM)-中国科学院国家授时中心(National Time Service Center, NTSC)卫星双向时间传递链路作为待优化链路,以NTSC、NIM以及德国联邦物理技术研究所(Physikalisch-Technische Bundesanstalt,PTB)之间的TWSTFT链路组成优化网络,对优化网络的组网方法和条件平差模型进行实验验证.结果表明,平差后待优化链路短期稳定度得到了改善,同时其受周日效应的影响降低了约24.6%.使用该方法,能够有效提高待优化链路的时间传递性能.     

Biomarker records of phytoplankton productivity and community structure changes during the last 14000 years in the mud area southwest off Cheju Island, East China Sea

The ecological environment in the East China Sea (ECS) and the Yellow Sea (YS) has changed significantly due to sea-level rising and the Kuroshio incursion since the last deglaciation. In this study, biomarker records of core F10B from the mud area southwest off Cheju Island (MSWCI) were generated to evaluate phytoplankton productivity and community structure changes in response to environmental evolution during the last 14 kyr. The contents of diatom, dinoflagellate and haptophyte biomarkers (brassicasterol, dinosterol and C₃₇ alkenones) display similar trends, with increasing phytoplankton productivity during the last 14 kyr due to the increased influences of the Kuroshio, and especially due to the eddy-induced upwelling during the late Holocene. On the other hand, the contents of terrestrial biomarkers (C₂₈ +C₃₀ +C₃₂ n-alkanols) and terrestrial organic matter (TOM) proxies (TMBR’ and BIT) all reveal decreasing TOM input into the area around the sampling site for the 14 kyr, mostly due to sea-level rising. Phytoplankton biomarker ratios reveal a shift from a haptophyte-dominated community at 6.2–2.5 kyr BP to a diatom-dominated community at 2.5–1.45 kyr BP, likely caused by a stronger cold eddy circulation system at 2.5–1.45 kyr BP in the MSWCI.