基于三维荧光光谱-平行因子技术联用的浮游藻化学分类学技术研究

An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis(PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix(EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis(BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM–PARAFAC–CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios(CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ.     

基于UTC（NTSC）的GPS定时接收机时延测量

GPS提供了一种精密定时的方法,但接收机绝对时延的测量是一个难题,现有的测量方法成本高且难以操作,因而提出了一种GPS定时接收机时延测量的简单方法。这种方法根据国家授时中心保持的时间尺度UTC（NTSC）与GPS定时接收机输出的秒脉冲信号（1PPS）的比对结果,利用UTC（NTSC）的国际比对数据以及UTC与GPST的时差数据,可以测量GPS定时接收机时延,该方法简单易行。最后,对这种方法的测量误差进行了分析。     

渤、黄、东海潮汐、潮流的数值模拟与研究

基于FVCOM海洋数值模式,采用高分辨率的三角形网格,对渤、黄、东海的潮汐、潮流进行数值模拟,并通过比较120个沿岸验潮站和14个潮流观测站的实测与模拟结果进行模型验证,两者符合较好。根据模拟结果,给出了四个主要分潮的潮汐同潮图和5m层潮流最大流速及最大潮流同潮时分布。渤、黄、东海共有5个半日分潮和3个全日分潮的独立旋转潮波系统,且都呈逆时针方向旋转;半日潮流和全日潮流各有12个圆流点;在冲绳岛和奄美岛两侧的4个半日潮流圆流点分别呈对称分布,其中有3个为本文首次给出;在日本九州岛西侧还新给出2个全日潮流圆流点。有关它们的存在性需要实测资料的进一步检验。     

2004年春季东海赤潮高发区COD分布及其与赤潮关系的初步研究

2004年4～5月初在东海赤潮高发区暴发的特大规模原甲藻赤潮前期和暴发初期对该海域进行的现场调查,并对该海域COD的分布特征进行了探讨。结果表明,赤潮暴发前COD为0．295-1.836mg／L,主要受陆源输入影响。根据其在局部海区底层出现的异常升高结合其他参数分析可对特定海区潜在赤潮暴发的可能性进行评估。赤潮暴发时COD为0．36～3．14mg／L,表层和中层与叶绿素存在显著正相关关系,表明其主要受生物影响。富营养化指数表明赤潮暴发前近一半海域已经处于富营养化状态,但COD对富营养化的贡献不如营养盐重要。     

营养限制对三种浮游动物的影响

Nutrient imbalance—a mismatch in nutrient ratios between the available food supply and the demands of consumers—has the potential to be transported up food chains,exposing zooplankton to nutrient limitations.In this study,the response of Calanus sinicus(copepod),Moina mongolica(cladocera),and Brachionus plicatilis(rotifer) to nutrient-limited(no-limited,P-limited,and N-limited) food were evaluated from the perspective of growth,reproduction,and stoichiometric homoeostasis.The results indicated that the growth of three species was suppressed under nutrient-limited(especially P-limited) conditions.However,the effect of nutrient limitations on their reproduction was species-specific.For C.sinicus,the dietary UFA(unsaturated fatty acid) as a major phospholipid component affected their egg production and total FA supporting energy promoted the hatchability of eggs.Furthermore,excess carbon in the diet promoted egg production but reduced hatching success.For M.mongolica,nutritional(P and UFA) and energy(total FA) support affected their fecundity.B.plicatilis fecundity exhibited the same pattern of growth(no-limitedN-limitedP-limited).In terms of stoichiometric homeostasis,B.plicatilis' s elemental compositions were less likely to be affected by nutrient limitations than M.mongolica.We suggest that the effects of nutrient imbalances could potentially become an evolutionary force affecting ecosystem structure and stability in eutrophic waters.     

马蔺根系固土护坡效应研究

研究以青藏高原东北部观赏地被植物马蔺为研究对象,通过野外调查和材料力学、土力学试验,定量评价了野生马蔺植株和根系生长特征、单根抗拉特性以及根系对土体抗剪强度的增强效应。结果表明,马蔺平均株高、冠幅和植株密度分别为(75.22±11.40)cm、(106.09±25.62)cm和(1.51±0.55)株/m²;马蔺标准株根系分布深度可达50 cm,主要分布在0～30 cm深度范围内,根幅约40～50 cm。马蔺根径为0.20～0.70 mm,单根抗拉力、抗拉强度和延伸率平均值分别为(7.94±2.91)N,(46.30±11.06)MPa和54.17%±17.08%。随着根径的增大,单根抗拉力和单根抗拉强度分别呈幂函数增大和幂函数降低趋势,单根极限延伸率随着根径的增大呈逐渐增大变化趋势,但二者之间未表现出相对显著性的关系。马蔺根系对土体黏聚力和内摩擦角均有增强作用,可分别显著增强0～30 cm和0～10 cm深度范围内土体黏聚力和内摩擦角,增长率分别为9.48%～17.40%和7.62%。本文研究成果对马蔺用于坡面水土流失、浅层滑坡等地质灾害现象的生态防护工作,具有...     

青海省气候异常偏暖的成因分析

在全球气候变化的背景下,1998年青海省创下了有气象记录以来最暖的一年。从资料分析得知:全球气候变暖及1998年全球范围内部分地区环流异常,以及近几年来省内干旱、水土流失、土地荒漠化等因素是导致1998年我省异常偏暖气候的主要成因。     

喷涂聚脲弹性体技术在海港码头预应力大管桩上的应用

针对北仑电厂预应力大管桩表面潮湿,需候潮作业,施工条件复杂,施工作业时间短的特点,从表面处理、参数的设定、喷涂施工、质量控制及验收和存在问题及解决方案等方面,介绍了喷涂聚脲弹性体技术在已建海港码头预应力大管桩表面防腐保护工程中的应用情况。可为类似结构物的设计、施工、维护及管理提供参考。     

水中矿物油荧光特性实验研究

为了实现现场测量地表水中矿物油含量的全光纤荧光计的研制。采用荧光光谱测量分析方法 ,于 1 999年 1 0月— 2 0 0 0年 5月 ,采集了国内外不同的地区原油和成品油共 2 3种 ,用1 4种不同波长的激发光激励 ,进行荧光光谱特性测量实验研究。同时 ,对不同浓度的矿物油、水混合液也进行了测量实验 ,得出其最低检测浓度为 0 .0 0 5mg/L ;测量了青岛近岸海域表层受油污染海水的含油量为 0 .0 1～ 0 .2 2mg/L ;此外对水中矿物油受激荧光发射原理也作了简述。并对水中矿物油荧光光谱特性、浓度与荧光强度的关系 ,以及为研制现场测量水中矿物油浓度的全光纤荧光计的可能性作出了结论性的意见     

江苏淤泥质潮滩对海平面变化的形态响应

通过对江苏滨海平原淤泥质潮滩1980年以来19个固定潮滩断面112个测次滩面高程测量的统计分析，探讨典型淤泥质潮滩剖面形态对海平面变化的形态响应过程。结果表明，典型淤涨岸段海平面上升，多年平均潮位线以上滩面仍将淤积加高，但淤高幅度除多年平均高潮线附近滩面相对较大外，其余均较小，表明随海平面上升该滩带总体淤积速率将趋于减小；与此相反，多年平均潮位线以下滩面则趋于蚀低，且侵蚀强度较大，表明该滩带的侵蚀有加剧趋向，最终滩面总体形态将因上带不断淤高和下带不断蚀低而逐渐变陡，剖面上凸形态的曲率不断加大。曲型侵蚀岸段，海平面上升的效应则相反，海平面上升，多年平均潮位线以上滩面强烈蚀低；而多年平均潮位线以下滩面则强烈淤积加高，剖面的上凹形态最终将因上带不断蚀低和下带不断淤高而趋于平直。