Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus).

DNA from five tumors, three other-injured livers and two normal liver tissue samples from the European flounder were analyzed for mutations in exons 5-8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-ras proto-oncogenes. No tumor-specific mutations were identified by direct sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need for more extensive analyses of flounder liver tumor samples for ras or p53 mutations, other cancer-related genes should be investigated.     

Improved Resolution of Ambient Flow through Fractured Rock with Temperature Logs

In contaminant hydrogeology, investigations at fractured rock sites are typically undertaken to improve understanding of the fracture networks and associated groundwater flow that govern past and/or future contaminant transport. Conventional hydrogeologic, geophysical, and hydrophysical techniques used to develop a conceptual model are often implemented in open boreholes under conditions of cross-connected flow. A new approach using high-resolution temperature (±0.001°C) profiles measured within static water columns of boreholes sealed using continuous, water-inflated, flexible liners (FLUTe™) identifies hydraulically active fractures under ambient (natural) groundwater flow conditions. The value of this approach is assessed by comparisons of temperature profiles from holes (100 to 200 m deep) with and without liners at four contaminated sites with distinctly different hydrogeologic conditions. The results from the lined holes consistently show many more hydraulically active fractures than the open-hole profiles, in which the influence of vertical flow through the borehole between a few fractures masks important intermediary flow zones. Temperature measurements in temporarily sealed boreholes not only improve the sensitivity and accuracy of identifying hydraulically active fractures under ambient conditions but also offer new insights regarding previously unresolvable flow distributions in fractured rock systems, while leaving the borehole available for other forms of testing and monitoring device installation.     

Diatom evidence for autochthonous artifact deposition in the Valsequillo region, Puebla, Mexico during the Sangamonian (sensu lato = 80,000 to ca. 220,000 yr BP and Illinoian (220,000 to 430,000 yr BP))

Fossil diatoms in the Valsequillo area are important in supplying adequate paleoecological evidence for the in situ deposition (in the absence of strong water currents necessary for the displacement and redeposition) of artifacts as large as those at the Hueyatlaco Archaeological Site. The paleoecology of lacustrine diatom-bearing samples from four nearby localities in the Valsequillo region all correlated with numerous diatomaceous samples from the Hueyatlaco Site (Puebla, Mexico) and indicate an autochthonous deposition of the artifacts at that site. This correlative evidence is consistent with a deposition in Sangamonian to Illinoian time and is based on the relationships of percentages of taxa in categories of the current, pH, and halobian spectra in six lines of correlation of samples between the Hueyatlaco Site and the four localities.     

Temporal variability in the maternal environment and its effect on seed size and seed quality in Flourensia cernua DC. (Asteraceae).

The biological phenomenon commonly referred to as maternal effect occurs whenever the environment exerts an influence upon the mother that is later expressed in characteristics of her offsprings. For example, the environmental conditions experienced by a mother plant during flowering and fruiting can modify the quality of her descendants (seeds), and even interrupt their development. Between-year variation in minimum temperature and the classic, between-year variation in precipitation represent an unpredictable environment for winter blooming plants in arid zones. In this study, we investigated the effect of maternal environment (temperature and precipitation) on seed size and seed quality in F. cernua, over a 5-year period. Results indicated that the proportion of filled seeds, as well as their size, increased with higher absolute minimum temperatures during seed formation, and that, to a lesser extent, both the precipitation occurred during the same period and the annual precipitation also have a positive influence on seed size. In this way, environmental conditions prevailing during seed formation exerted a strong maternal effect on the size and quality of seeds produced and, probably also, in the developmental possibilities of F. cernua seedlings. This effect was subject to important, between-year variation.     

Vertical migration ofcentropages typicus (copepoda) in Chesapeake Bay,with some thoughts on migration studies

Centropages typicus was collected at two hour intervals over a diel cycle at a station near the Chesapeake Bay mouth. The species migrated upward at dusk and downward at dawn. Neither light, temperature, salinity, nor density appeared to cue the migration. Attention is drawn to the need for information on the extent and regularity of migration by individual organisms, and how the lack of such information may lead to poor interpretations of migration data.     

Impact of Sea-level Rise and Storm Surges on a Coastal Community

A technique to evaluate the risk of storm tides (the combination of a storm surge and tide) under present and enhanced greenhouse conditions has been applied to Cairns on the north-eastern Australian coast. The technique combines a statistical model for cyclone occurrence with a state-of-the-art storm surge inundation model and involves the random generation of a large number of storm tide simulations. The set of simulations constitutes a synthetic record of extreme sea-level events that can be analysed to produce storm tide return periods. The use of a dynamic storm surge model with overland flooding capability means that the spatial extent of flooding is also implicitly modelled. The technique has the advantage that it can readily be modified to include projected changes to cyclone behaviour due to the enhanced greenhouse effect. Sea-level heights in the current climate for return periods of 50, 100, 500 and 1000 years have been determined to be 2.0 m, 2.3 m, 3.0 m and 3.4 m respectively. In an enhanced greenhouse climate (around 2050), projected increases in cyclone intensity and mean sea-level see these heights increase to 2.4 m, 2.8 m, 3.8 m and 4.2 m respectively. The average area inundated by events with a return period greater than 100 years is found to more than double under enhanced greenhouse conditions.     

First data on U-Pb SHRIMP-II isotopic dating based on zircons of ash sediments from Cenozoic depressions in Southwest of Primorie

U-Pb (SHRIMP-II) isotopic dating has been conducted for zircons of the ash sediments of the Ust’-Suifun Suite, which was the final stage of Late Cenozoic explosive volcanism in Southwest Primorskii krai. These pyroclastic units are widespread within sediments that fill in Cenozoic depressions including large coalfields. The concordant dates (23.7–24.6 Ma) are in line with the results of the K-Ar determinations for volcanic tephra (23.6–27.1 Ma) and correspond to the beginning of the active phase of spreading and taphrogenesis in the neighboring Trench of the Sea of Japan. These processes started as early as the Eocene and are reflected in the continental vicinity with the formation of riftogenic depressions and the occurrence of a peculiar gas volcanism.     

A theoretical model of Fanaroff-Riley I jets

A fluid model of the jets in Fanaroff-Riley I class, for extended extragalactic radio sources, based on the idea that they are turbulent low Mach number supersonic flows consisting of relativistic and nonrelativistic plasma, has been developed. Mean flow approach and modified mixing length theory by Prandtl are used to obtain a corresponding mathematical tool. It is assumed that magnetic field is weak. Boundary conditions concerned the problem of the jet propagation through a non-uniform atmospheres of an elliptical galaxies are considered. Short discussion of the observational data determining the choice of the input parameters of the jets is given.     

The British Tertiary igneous province: palaeomagnetism of the dyke swarm along the Sleat coast of Skye

Summary. A palaeomagnetic investigation has been made of a swarm of more than 400 dykes along the south coast of Skye, Scotland, by the Sound of Sleat.
Seven red lamprophyre dykes have palaeomagnetic directions inconsistent with Tertiary age, and not inconsistent with their previously held Caledonian age. The remaining 409 dykes have palaeomagnetic directions that are consistent with a Lower Tertiary age. We present evidence suggesting that the Tertiary dykes might have been emplaced during a short time, over which the geomagnetic polarity occupied as few as three polarity intervals (NRN or RNR).
Certain 'intermediate' directions of magnetization have also been found, and are presented here.