Axenic culture of microalgae
Chlorella vulgaris ATCC
® 13482 and
Scenedesmus obliquus FACHB 417 was used for phycoremediation of primary municipal wastewater. The main aim of this study was to measure the effects of normal air and CO
2-augmented air on the removal efficacy of nutrients (ammonia N and phosphate P) from municipal wastewater by the two microalgae. Batch experiments were carried out in cylindrical glass bottles of 1 L working volume at 25 °C and cool fluorescent light of 6500 lux maintaining 14/10 h of light/dark cycle with normal air supplied at 0.2 L min
?1 per liter of the liquid for both algal strains for the experimental period. In the next set of experiments, the treatment process was enhanced by using 1, 2 and 5% CO
2/air (vol./vol.) supply into microalgal cultures. The enrichment of inlet air with CO
2 was found to be beneficial. The maximum removal of 76.3 and 76% COD, 94.2 and 92.6% ammonia, and 94.8 and 93.1% phosphate after a period of 10 days was reported for
C. vulgaris and
S. obliquus, respectively, with 5% CO
2/air supply. Comparing the two microalgae, maximum removal rates of ammonia and phosphate by
C. vulgaris were 4.12 and 1.75 mg L
?1 day
?1, respectively, at 5% CO
2/air supply. From kinetic study data, it was found that the specific rates of phosphate utilization (
q phsophate) by
C. vulgaris and
S. obliquus at 5% CO
2/air supply were 1.98 and 2.11 day
?1, respectively. Scale-up estimation of a reactor removing phosphate (the criteria pollutant) from 50 MLD wastewater influent was also done.
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