In situ velocity profiles in gassy sediments: Kiel Bay

Acoustic behavior of gas-bearing sediments is significantly different from that of gas-free sediments. In situ velocity profiles and acoustic signal characteristics in gas-bearing sediments of the upper several meters of the sea floor in Kiel Bay are presented in this study. Observed velocities in gas-bearing sediments are both higher and lower than those of the gas-free sediments. Small amounts of gas appear to cause signal reverberation without much attenuation. whereas large amounts of gas cause substantial attenuation.     

Numerical experiments on the four-dimensional assimilation of the observation data derived in the Black Sea in June 1984 on the basis of a numerical energy-balanced model

A procedure for the four-dimensional (4D) analysis of the hydrophysical fields in the Black Sea with an assimilation of the temperature and salinity (T, S) data was realized on the basis of a numerical model which involves the primitive equations of motion, and the heat and salt advection equations. Two experiments were carried out which differed by the observation data assimilation procedure. Analysis has shown that the observation data assimilation procedure realized using the energy-balanced model allows the reproduction of some synoptic features of the circulation in the Black Sea. A comparison of two computations demonstrates the efficiency of assimilating the measurement data on the basis of the 4D analysis as compared with the sequential objective analysis.Translated by Mikhail M. Trufanov.     

Serum biochemical and cellular responses to experimental cupric ion challenge in mussels

Our intent has been to develop rapid and sensitive bioassay procedures for laboratory and field applications based on hemolymph factors in animals such as the bay mussel, Mytilus edulis. We found that mussel hemolymph could be easily and repeatedly sampled from individual animals in sufficient amounts to allow analyses for lysozyme, proteolytic activity, total protein and hemocyte counts. In static tests there seemed to be graded responses indicating release of increasing amounts of the above factors into the hemolymph in roughly direct proportion to Cu²⁺ concentration at lethal and sublethal levels. We interpreted this to be evidence for increased lysosomal and cellular disruption as cupric ion exposure was increased or prolonged. At the same time, Cu²⁺ concentrations in the mussel hemolymph increased up to an order of magnitude above ambient in the range of 26 to 267 ppb. We felt that this was evidence for the release of Cu-binding proteins into the mussel hemolymph. Simultaneously with the above events the serum granulocytes increased in numbers above control values by factors of 3 or 4 in response to copper challenge while macrophage (terminology of Moore & Lowe, 1977) counts, including controls, declined precipitously.     

In situ tests for ice strength measurements

Ice effects have caused extensive damage. Enormous forces have been recorded. On the other hand, ice has been used as a structural material under various situations. To use ice beneficially and to assess the danger of ice forces, it is necessary to know the mechanical properties of ice.Results from the large number of ice tests carried out in laboratories show the difficulties of sampling and testing procedures. Most of the tests have not reproduced faithfully enough the conditions existing in nature. To obtain adequate results, laborious and slow samplings and preparations of test specimens have been necessary. Recently, extensive use has been made of in situ tests in order to reduce the number of factors whixh can induce errors in measurements. Large scale tests have simulated actual structures. General ice properties have become available from small-scale in situ tests. Since these tests give rapid results, a large number are already available with a good coverage of field conditions.A theoretical basis has been devised for these in situ tests which measure essentially the unconfined and confined compressive strengths of ice, and practical devices have been developed to execute these tests. A correlation of measurements from laboratory tests and in situ tests has established a link between natural and artificial environments. Brittle elastic and clastic theories have been applied, to find the best correlations for ice tests. Test results have been related to the mechanical properties as used in engineering practice.A very summary conclusion of results is that cold sea ice and cold fresh water ice are both very strong. However, depending on the load rate, ice can be quite brittle, which mitigates dangerous effects from ice action against massive structures.     

Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach

An integrate approach will be discussed for investigations on oxidative stress in xenobiotic toxicity. While the analysis of individual antioxidants is useful for their sensitivity and to understand the mode of action of a stressor, the integration with the analysis of the total antioxidant capacity provides a more holistic assessment of the overall biological significance of such variations. TOSC has a greater predictive value on the health condition of the organisms and allows to discriminate the different role of specific ROS in oxidative stress syndrome.