Diversity of marine and brackish water nitrite-oxidizing consortia developed for activating nitrifying bioreactors in aquaculture

Nitrite is a well-known toxicant in aquaculture, produced as intermediate in nitrification. Two nitrite-oxidizing bacterial consortia, one from marine environment and the other from brackish water, were developed by enrichment technique at National Centre for Aquatic Animal Health, for removal of nitrite from recirculating aquaculture systems. In the present study, bacterial diversity of the consortia was assessed based on 16S ribosomal RNA and the functional gene analysis. Clone libraries of 16S ribosomal RNA gene and nitrite oxidoreductase A gene were constructed, and amplified ribosomal DNA restriction analysis was carried out to cluster the clones. Dendrograms generated through molecular characterization showed 29 and 27 clusters in marine and brackish water consortia, respectively. Phylogenetic analyses of representative clones from each cluster depicted profound diversity in the consortia consisting autotrophic nitrifiers belonging to Proteobacteria, anaerobic ammonia oxidizers, Actinobacteria, Bacteroidetes and heterotrophic denitrifiers. Functional gene analysis corroborated with the presence of specific nitrite oxidizers. Quantitative polymerase chain reaction showed the abundance of nitrite oxidizers in the order of 1.51 ± 0.38 × 10⁹/g and 4.88 ± 0.42 × 10⁷/g in marine and brackish water consortia, respectively. Diversity indices and pattern of distribution of organisms within the consortia were analyzed using Geneious, VITCOMIC, Mega 5 and Primer software. The marine nitrite-oxidizing consortium showed higher Shannon–Wiener diversity and mean population diversity than brackish water consortium, suggesting that the former was having more diverse flora and higher potential to be used as startup cultures for activating nitrifying bioreactors subsequent to acclimatization to the required salinity.     

Potency of constructed wetlands for deportation of pathogens index from rural,urban and industrial wastewater

Pathogen removal is essential for wastewater treatment and its potential reuse in agriculture. Three field-scale wastewater treatment systems consisting of free surface flow were operated around 1.5 years receiving water from urban domestic, rural domestic and industrial sources. The study was conducted to evaluate seasonal performance of constructed wetland systems in removing Escherichia coli, Enterococci and total coliforms under continuous hydraulic flow. Results displayed that all three wetlands gain recognition in removing pathogen load with high removal efficacy till water reaches output ports. Removal efficiencies were even higher, 66–93, 78–92 and 80–94% for E. coli, Enterococci and total coliforms, respectively, within constructed wetlands. Remarkably at shorter temporal scales in CW-A, greater homogeneity of pathogen concentrations was assessed at wetland outlet sites. In outlet ports, results displayed a highly effective removal of E. coli concentration 80–90% (June 2015), 86–92% (October 2015) and 79–92% (February 2016), Enterococci 80–94% (June 2015), 83–94% (October 2015) and 80–94% (February 2016) and total coliforms 85–93% (June 2015), 87–95% (October 2015) and 88–96% (February 2016). Positive correlation was observed between bacterial indicators (E. coli–Enterococci, r = 0.038; p < 0.01 and E. coli–total coliforms, r = 0.142; p < 0.01). Removal of bacterial indicators in constructed wetland was also displayed by PCA in which three-component analysis of variance was 98.39% and showed a clear decrease in measured parameter gradients toward samples from outlet ports. Constructed wetlands provide cost-effective treatment systems for reducing the pathogen load in wastewater in variable agro-climatic conditions and thus improve water quality.     

Management of shock loads wastewater produced from water heaters industry

Water heater manufacturing represents one sector of household electrical appliance industry. It includes several batch processes which resulted in a highly polluted wastewater as shock loads. The objective of this study was to manage the shock loads wastewater with a simple and cost-effective approach prior to final discharge into municipality. To achieve this objective, two approaches were studied. The first approach was the chemical treatment of the accumulated shock loads wastewater using alum and an anionic polymer. Although this approach produced a very high-quality effluent, it was economically and technically infeasible. The second approach was a controlled release of the shock loads to the normal daily discharge in a way that guarantees the compliance of the end-off-pipe with the National Regulatory Standards. This solution required establishment of an equalization tank for normal daily flow and a holding tank for controlled release of the shock loads. Mathematical calculations were carried out to determine the most violating parameters in order to calculate the mixing ratio the of shock loads with the normal daily flow. Full engineering design of the proposed solution was carried out. This approach was implemented and proved to be simple, easy to operate, cost-effective and can be replicated in similar batch processing manufacturing plants.     

Water stress index and its implication for agricultural land-use policy in Thailand

Land-use change and expansion for agriculture significantly affect freshwater resource availability which in turn results in different levels of water scarcity and competition among users in different areas and time. The study uses the water stress index as an indicator for determining the potential impact of water use considering water deprivation potential. Considering the case of Thailand, the temporal aspects of water withdrawal and availability have been analyzed and characterized as the monthly water stress index for the 25 watersheds in the country. Wide variations of monthly water stress index are obtained; extreme water stress is observed in the areas of the Chao Phraya and Tha Chin watersheds (the central region) during the dry season because of the large areas cultivating second rice (rice planted in dry season). The target of the Thai government on agricultural land-use change and zoning toward the conversion of about 0.37 M·ha of the upland paddy fields with low productivity to sugarcane was evaluated showing serious implications on the monthly water stress index of the relevant watersheds and the water scarcity footprint potentials of rice and sugarcane production. The results reveal that proper policy can help reduce the amount of water requirement for agriculture in June, July, August and September by about 60–220 M·m³, which in turn results in the decrease in monthly water stress index values. Nevertheless, appropriate measures of water resource management for agriculture still need to be designed to avoid water competition as well as protect the ecosystem.     

An orthotropic interface damage model for simulating drying processes in soils

The study of drying process in soils has received an increased attention in the last few years. This is very complex phenomenon that generally leads to the formation and propagation of desiccation cracks in the soil mass. In recent engineering applications, high aspect ratio elements have proved to be well suited to tackle this type of problem using finite elements. However, the modeling of interfaces between materials with orthotropic properties that generally exist in this type of problem using standard (isotropic) constitutive model is very complex and challenging in terms of the mesh generation, leading to very fine meshes that are intensive CPU demanding. A novel orthotropic interface mechanical model based on damage mechanics and capable of dealing with interfaces between materials in which the strength depends on the direction of analysis is proposed in this paper. The complete mathematical formulation is presented together with the algorithm suggested for its numerical implementation. Some simple yet challenging synthetic benchmarks are analyzed to explore the model capabilities. Laboratory tests using different textures at the contact surface between materials were conducted to evaluate the strengths of the interface in different directions. These experiments were then used to validate the proposed model. Finally, the approach is applied to simulate an actual desiccation test involving an orthotropic contact surface. In all the application cases the performance of the model was very satisfactory.     

Anisotropic Continuous-in-Scale Universal Multifractal Cascades: Simulation,Analysis and Correction Methods

Continuous-in-scale multifractal cascades has long been an attractive choice for mathematically modeling turbulent and turbulent-like geophysical fields. These fields are usually anisotropic as they are subject to both stratification and rotation, thereby questioning the isotropy assumption often made to model them. The self-affine and generalized scale invariance approaches to scaling are used here to introduce anisotropy in such models. These anisotropic simulations have (1) unresolved large-scale features and (2) statistics that deviate from the desired power-law scaling mainly in the small scales. The former issue is solved via nesting, whereas the latter is attempted to be overcome using singularity correction methods. While earlier studies have proposed isotropic correction methods, here they have been generalized to correct anisotropic simulations. These singularity corrections seem to improve the small-scale statistical properties of mildly anisotropic simulations; nesting, on the other hand, appears to enhance statistics over almost all scales even for strongly anisotropic simulations. Both the correction and nesting techniques lead to a reduction in computational time and memory usage suggesting that nested singularity-corrected cascades offer a better framework for quantitatively modeling the atmosphere, ocean, solid earth, and associated fields.     

Calorimetric determination of the standard enthalpy of formation of stannite,Cu<Subscript>2</Subscript>FeSnS<Subscript>4</Subscript>

The standard enthalpy of formation of stannite (Cu₂FeSnS₄) was calculated from the calorimetric measurements of the reactions of its formation from simple synthetic sulfides: Cu₂S + FeS₂ + SnS → Cu₂FeSnS₄ and ₂CuS + FeS + SnS → Cu₂FeSnS₄. Using published data for the binary sulfides, the standard enthalpy of formation of stannite from the elements was determined as ΔfH°_298.15(Cu₂FeSnS₄) =–(417.28 ± 2.28) kJ mol^–1.