Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Investigation of internal waves generated by a diurnal barotropic tide in the vicinity of an oceanic ridge

Internal waves occurring near an oceanic ridge as a result of impingement of a barotropic tide are scrutinized in terms of the long theory for long waves. The ocean is assumed to be double-layered, the relief continuously changing, and the tidal wave on-running at an arbitrary angle. The dependences of the wave amplitudes and horizontal velocities on the ridge's latitude, the angle of tide running, and the depth of the density jump layer are obtained. Similar investigations for the model bottom relief have been conducted in refs 1 and 3.Translated by Vladimir A. Puchkin.     

The choice of a dynamico-stochastic model for ocean monitoring — A compromise between the accuracy and cost of computations

In this paper we compare, two dynamico-stochastic models (DSMs) with different hydrodynamic parts, designed to study a large-scale North Atlantic region. As a performance criterion, RMS errors (over the horizon) in density and velocity field reconstruction are used. It has been shown that in some cases it seems rational to use DSMs with simplified hydrodynamic parts. This helps to expedite the computations and to reduce the related costs without much detriment to the quality of hydrophysical field simulation.UDK 551.465.001.573(261.1).Translated by Vladimir A. Puchkin.     

Preservation conditions and the use of sediment pigments as a tool for recent ecological reconstruction in four Northern European estuaries

Down-core sediment pigment concentrations from four Northern European estuaries were measured using high-performance liquid chromatography (HPLC) to investigate phytoplankton community structure and preservation conditions over the last ca. 100 years where all sites have experienced different levels of eutrophication. Phytoplankton pigments have been shown to be useful biomarkers for phytoplankton community structure and abundance due to their taxonomic specificity. The pigment concentrations and sediment pigment inventory showed large variation between the four sites. Concentrations ranged from more than 6000 nmol/g OC to less than 100 nmol/g OC and the inventory integrated over the top 10 cm from more than 300 nmol/cm² to less than 30 nmol/cm² for total identified pigments. Good pigment preservation in Mariager Fjord (Denmark) reflected the almost permanently anoxic conditions. Pigments in Laajalahti (Finland) showed peak concentrations around the time of highest nitrogen loading events known from historical and modelled records over the past 100 years. In contrast, poor down-core preservation of pigments (especially carotenoids) was observed in the Ems-Dollard (The Netherlands) and Himmerfjärden (Sweden) estuaries. The Ems-Dollard site is an intertidal mudflat that experiences daily exposure to light and air, which enhances pigment degradation. In Himmerfjärden, resuspension is an important process affecting both the sedimentation rate and degradation properties. The different preservation conditions at the four sites were supported by the differences in two degradation indicators; the ratio of pheopigment-a to chlorophyll-a and total carotenoids to total pigments. Class-specific carotenoid pigments represented the dominant algal groups reported from each site, however, no distinct down-core changes in the pigment composition were observed at any of the four sites. This indicated that changes in plankton community structure on the group level have been limited over this time period or masked by low preservation of pigments.     

Wave interaction with a submarine pipeline in clayey soil due to random waves

The wave pressure and uplift force due to random waves on a submarine pipeline (resting on bed, partially buried and fully buried) in clayey soil are measured. The influence of various parameters viz., wave period, wave height, water depth, burial depth and consistency index of the soil on wave pressures around and uplift force on the submarine pipeline was investigated. The wave pressures were measured at three locations around the submarine pipeline (each at 120° to the adjacent one). It is found that the wave pressure and uplift force spectrum at high consistency index of the soil is smaller compared to that of low consistency index. Just burying the pipeline (e/D=1.0) in clayey soil reduces the uplift force to less than 60% of the force experienced by a pipeline resting on the seabed (e/D=0.0) for I_c=0.33.     

Pressure prediction in the Bullwinkle Basin through petrophysics and flow modeling (Green Canyon 65, Gulf of Mexico)

Reservoir pressures within the Bullwinkle minibasin (Green Canyon 65, Gulf of Mexico continental slope) increase at a hydrostatic gradient whereas pressures predicted from porosity within mudstones bounding these reservoirs increase at a lithostatic gradient: they are equal at a depth 1/3 of the way down from the crest of the structure. Two- and three-dimensional steady-state flow models demonstrate that bowl-shaped structures will have lower pressures than equivalent two-dimensional structures and that if a low permeability salt layer underlies the basin, the pressure is reduced. We conclude that at Bullwinkle, pressure is reduced due to an underlying salt body and the bowl-shape of the basin. A geometric approach to predict sandstone pressure is to assume that the reservoir pressure equals the area-weighted average of the mudstone pressure. When the mudstone pressure gradient is constant, as at Bullwinkle, the reservoir pressure equals the mudstone pressure at the average depth (centroid) of the reservoir.     

Jason-1: Assessment of the System Performances

On 7 December 2001, Jason-1 was successfully launched by a Boeing Delta II rocket from the Vandenberg Air Force Base, California. The Jason-1 satellite will maintain the high accuracy altimeter service provided since 1992 by TOPEX/Poseidon (T/P), ensuring the continuity in observing and monitoring the Ocean Dynamics (intraseasonal to interannual changes, mean sea level, tides, etc.). Despite one-fourth the mass and power, the Jason-1 system has been designed to have basically the same performance as T/P, measuring sea surface topography at a centimetric level. This new CNES/NASA mission also provides near real-time data for sea state and ocean forecast. The first two months of the Jason-1 mission have been dedicated to the assessment of the overall system. The goals of this assessment phase were:

1. To assess the behavior of the spacecraft at the platform and payload levels (Jason-1 being the first program to call on the PROTEUS versatile multimission platform for Low and Medium Earth Orbit Missions developed in partnership between Alcatel Space and CNES);

2. To verify that platform performance requirements are met with respect to Jason-1 requirements;

3. To verify that payload instruments performance requirements evaluated at instrument level are met;

4. To assess the performance of the Jason-1 Ground System.

This article will display the main outputs of the assessment of the system. It will demonstrate that all the elements of the onboard and ground systems are within the specifications. Provision of data to the Jason-1 Science Working Team started at the end of March 2002. This is the goal of a six-month phase after closure of the initial assessment phase to derive the error budget of the system in terms of altimetry user products.