Management of a shallow temperate estuary to control eutrophication: The effect of hydrodynamics on the system’s nutrient loading

The Mondego estuary, a shallow warm-temperate intertidal system located on the west coast of Portugal, has for some decades been under severe ecological stress, mainly caused by eutrophication. Water circulation in this system was, until 1998, mainly dependent on tides and on the freshwater input of a small tributary artificially controlled by a sluice. After 1998, the sluice opening was effectively minimised to reduce the nutrient loading, and the system hydrodynamics improved due to engineering work in the upstream areas. The objective of the present study was to evaluate the effect of the mitigation measures implemented in 1998. Changes to the hydrodynamics of the system were assessed using precipitation and salinity data in relation to the concentrations of dissolved inorganic nutrients, as well as the linkage between dissolved N:P ratios and the biological parameters (phytoplankton chlorophyll a concentrations, green macroalgal biomass and seagrass biomass). Two distinctive periods were compared, over a ten year period: from January 1993 to January 1997 and from January 1999 until January 2003. The effective reduction in the dissolved N:P atomic ratio from 37.7 to 13.2 after 1998 is a result of lowered ammonia, but not the oxidised forms of nitrogen (nitrate plus nitrite), or increased concentrations of dissolved inorganic phosphorus. Results suggest that the phytoplankton is not nutrient limited, yet maximum and mean biomass of green macroalgae was reduced by one order of magnitude after the mitigation measures. This suggests that besides lowering the water residence time of the system, macroalgal growth became nitrogen limited. In parallel to these changes the seagrass-covered area and biomass of Zostera noltii showed signs of recovery.     

Numerical Solution of Lock-Release Gravity Current with Viscous Self-Similar Regime

Lock-release gravity currents with a viscous self-similar regime are simulated by use of the renormalization group(RNG) k - ε model for Reynolds-stress closure. Besides the turbulent regime with initially a slumping phase of a conslant current front speed and later an inviseid self-similar phase of front speed decreasing as t^-1/3(where t is the time measured from release), the viseous self-similar regime is satisfactorily reproduced with front speed decreasing as t^-4/5,consistent with well known experimental observations.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

The optimum control of thruster system for dynamically positioned vessels

An offshore vessel with a dynamic positioning system (DP system) needs fast response to produce thrust to counteract the environmental forces acting on it for the purpose of maintaining its position and heading as close as possible to the working position. Therefore, quick and effective modulation of the thrust is the problem to determine the thrust and the rotation angle of the thruster devices of the ship. This paper presents an effective optimum control for a thruster system, using the sequential quadratic method to achieve economical and effective modulation of the thrust and the direction of the thruster. An optimum control study of a 2280 tons DP coring vessel with five rotary azimuth thruster marine positioning is studied in detail, which can quickly and exactly estimate the thrusts and angles of direction of all the thrusters. The results can provide a valuable thruster system for a dynamically positioned vessel.     

Calibration of JASON-1 Altimeter over Lake Erie

This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr.     

基于EOF分析对南海西北海域水体光谱特性的研究

This study presents an analysis of the spectral characteristics of remote sensing reflectance(R_(rs)) in northwestern South China Sea based on the in situ optical and water quality data for August 2018.R_(rs)was initially divided into four classes,classes A to D,using the max-classification algorithm,and the spectral properties of whole R_(rs) were characterized using the empirical orthogonal function(EOF) analysis.Subsequently,the dominant factors in each EOF mode were determined.The results indicated that more than 95% of the variances of R_(rs) are partly driven by the back-scattering characteristics of the suspended matter.The initial two EOF modes were well correlated with the total suspended matter and back-scattering coefficient.Furthermore,the first EOF modes of the four classes of R_(rs)(A-D R_(rs)-EOF_1) significantly contributed to the total variances of each R_(rs) class.In addition,the correlation coefficients between the amplitude factors of class A-D R_(rs)-EOF_1 and the variances of the relevant water quality and optical parameters were better than those of the unclassified ones.The spectral shape of class AR_(rs)-EOF_1 was governed by the absorption characteristic of chlorophyll a and colored dissolved organic matter(CDOM).The spectral shape of class B R_(rs)-EOF_1 was governed by the absorption characteristic of CDOM since it exhibited a high correlation with the absorption coefficient of CDOM(a_g(λ)),whereas the spectral shape of class C R_(rs)-EOF_1 was governed by the back-scattering characteristics but not affected by the suspended matter.The spectral shape of class D R_(rs)-EOF_1 exhibited a relatively good correlation with all the water quality parameters,which played a significant role in deciding its spectral shape.     

Effects of the water-soluble fraction of microbiologically or physically altered crude petroleum on the heterotrophic activity of marine bacteria

Crude petroleum was emulsified or degraded by marine microorganisms and physically altered by an artificial weathering process. Only the water-soluble fraction from emulsified crude petroleum inhibited glutamate uptake by marine microorganisms from South Carolina and Georgia coastal waters. Chloroform extraction recovered almost equal amounts of total hydrocarbon from each water-soluble fraction. Chromatographic data indicated that the composition of microbially emulsified petroleum was similar to that of microbially degraded petroleum. Emulsification of crude petroleum was only observed in summer water samples and did not consistently occur at a given location. Results of this study indicate that the process by which crude petroleum is altered will influence its toxicity to microbial heterotrophic activity.     

Observed foundation behaviour of concrete gravity platforms installed in the North Sea 1973–1978

Available measurements of foundation behaviour of the 13 concrete gravity platforms installed in the North Sea in the years 1973–1978 are presented. The measurements cover both the installation phase and the early part of the operation period. For the installation phase, measurements of dowel and skirt penetration resistance, base contact stresses, tilt, piping and erosion, are presented. For the operation period, the presentation includes measurements of settlements, pore water pressures, base contact stresses, permanent lateral displacements, cyclic displacements, dynamic behaviour and erosion. The measurements are used to back calculate soil properties and to evaluate the adequacy of the design procedures which have been used. The observations give valuable experience with respect to the foundation performance of offshore gravity platforms.