Numerical study on dry deposition processes in canopy layer

A coupling model between the canopy layer (CL) and atmospheric boundary layer (ABL) for the study of dry deposition velocity is developed. The model consists of six parts: chemical species conservation equation including absorptive factor; the species uptake action including detailed vertical variation of absorptive element in CL; momen-tum exchange in CL which is represented by a first-order closure momentum equation with an additional larger-scale diffusive term; momentum exchange in ABL which is described by a complete set of the ABL turbulent statistic parameters; absorptivity (or solubility or reflection) at the surface including effects of the physical and chemi-cal characters of the species, land type, seasonal and diurnal variations of the meteorological variables; and deposition velocity derived by distributions of the species with height in CL. Variational rules of the concentration and deposi-tion velocity with both height and time are simulated with the model for both corn and forest canopies. Results pre-dicted with the bulk deposition velocity derived in the paper consist well with experimental data.     

High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production

The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil production may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish (Myxine glutinosa) collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of Myxine contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed.     

赤道太平洋的环流

一.引 言 赤道太平洋上层海洋环流的主要特征已被建立十多年了。1942年斯维尔德鲁普等人描述了中太平洋的表面流:往西流的北和南赤道流以及往东流的北赤道逆流。随后,南赤道逆流也被用表面动力高度图表示出来(Reia,1961),Cromwell等人(1954)发现了赤道潜流,从而对上述环流的描述进行了主要修正。Hisard和Rual(1970)发现了赤道潜流下面     

缺少密度或孔隙度测定数据的块体堆积速率的计算

缺少密度或孔隙度测定数据的块体堆积速率的计算Ｔ．Ｊ．Ｓ．Ｓｙｋｅｓ等块体堆积速率（ＭＡＲ）计算已被应用于深海钻探计划（ＤＳＤＰ）和大洋钻探计划（ＯＤＰ）岩心，以研究大洋盆地的沉积物演化，或把ＭＡＲ数据分离成岩石学成分以决定方解石补偿深度（ＣＣＤ）的历...     

大视野水下激光观察系统

严重受限的水下观察是无人遥控潜不器作业中的一个问题。小型二极管激励绿激光器的问世，使人科重新对作为克服某些水下观察问题手段的同步体积扫描产生了兴趣。由于所用光学装置的原因，同步扫描的局限之一在于所能达到的视野深度有限。     

Use of immunofluorescence technique in cultured fibroblasts from Mediterranean cetaceans as new "in vitro" tool to investigate effects of environmental contaminants

The aim of the present study was to propose the immunofluorescence technique in cultured fibroblasts from Mediterranean cetaceans as a new "in vitro" tool to explore the susceptibility of these marine mammals to different xenobiotic compounds. The cell lines were cultured from integument biopsies of free-ranging and stranded cetaceans (dead within 12h). Using the indirect immunofluorescence assay, we detected endogenous proteins induced by different contaminants. Here we present the method used for qualitative and quantitative evaluation of cytochromes P450 (CYP1A1 and CYP2B) induced by some POPs (DDTs and PCBs) and emerging contaminants (PBDEs) in fibroblast cell cultures of striped dolphin (Stenella coeruleoalba) and bottlenose dolphin (Tursiops truncatus). Immunofluorescence was quantified with a specially designed Olympus macro, DetectIntZ. A major result was the possibility of using this "in vitro" assay to quantify induction of endogenous proteins.     

Anoxia over the western continental shelf of India: bacterial indications of intrinsic nitrification feeding denitrification

Studies on the Arabian Sea coastal anoxia have been of immense interest, but despite its ecological significance there is sparse understanding of the microbes involved. Hence, observations were carried out off Goa (15 degrees 30'N, 72 degrees 40'E to 15 degrees 30'N, 72 degrees 59'E) to understand the processes that mediate the changes in various inorganic nitrogen species in the water column during anoxia. Water column chemistry showed a clear distinct oxic environment in the month of April and anoxic condition in October. Our study based on microbial signatures indicated that oxygen deficit appeared as a well-defined nucleus almost 40 km away from the coast during the oxic period (April) and spreads there after to the entire water column synchronizing with the water chemistry. Striking results of net changes in inorganic nitrogen species in nitrification blocked and unblocked experimental systems show that denitrification is the predominant process in the water column consuming available nitrate ( approximately 0.5 microM) to near zero levels within approximately 72 h of incubation. These observations have been supported by concomitant increase in nitrite concentration ( approximately 4 microM). Similar studies on denitrification-blocked incubations, demonstrate the potential of nitrification to feed denitrification. Nitrification could contribute almost 4.5 microM to the total nitrate pool. It was found that the relation between ammonium and total dissolved inorganic nitrogen (DIN) pool (r=0.98, p<0.001, n=122) was significant compared to the latter with nitrite and nitrate. The occurrence of high ammonium under low phosphate conditions corroborates our observations that ammonium does not appear to be locked under low oxygen regimes. It is suggested that ammonium actively produced by detrital breakdown (ammonification) is efficiently consumed through nitrification process. The three processes in concert viz. ammonification, nitrification and denitrification appear to operate in more temporal and spatial proximity than hitherto appreciated in these systems and this gives additional cues on the absence of measurable nitrate at surface waters, which was earlier attributed only to efficient algal uptake. Hence we hypothesize that the alarming nitrous oxide input into the atmosphere could be due to high productivity driven tighter nitrification-denitrification coupling, rather than denitrification driven by extraneous nitrate.     

Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae Enteromorpha spp. from the Scheldt Estuary (SW Netherlands, Belgium) and Thermaikos Gulf (N Aegean Sea, Greece)

Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200microgL(-1)Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (gamma-glutamyl-cysteinyl)(n)-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH+0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16nmolSgdwt(-1) in controls (at 0.5micromolCdgdwt(-1)) to 179nmolSgdwt(-1) (at 1.9micromolCdgdwt(-1)) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH+0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9micromolZngdwt(-1)) to 0.26 (at 4.9nmolSgdwt(-1)) (at 0.5molCdgdwt(-1)). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress.     

Wave Interactions with Multiple Semi-Immersed Jarlan-Type Perforated Breakwaters

This study examines wave interactions with multiple semi-immersed Jarlan-type perforated breakwaters. A numerical model based on linear wave theory and an eigenfunction expansion method has been developed to study the hydrodynamic characteristics of breakwaters. The numerical results show a good agreement with previous analytical results and experimental data for limiting cases of double partially immersed impermeable walls and double and triple Jarlan-type breakwaters. The wave transmission coefficient C_T; reflection coefficient C_R, and energy dissipation coefficient C_E coefficients and the horizontal wave force exerted on the front and rear walls are examined. The results show that C_R reaches the maximum value when B/L = 0.46n while it is smallest when B/L=0.46n+0.24 (n=0, 1, 2,...). An economical triple semi-immersed Jarlan-type perforated breakwater can be designed with B/L = 0.25 and C_R and C_T ranging from 0.25 to 0.32 by choosing a relative draft d/h of 0.35 and a permeability parameter of the perforated front walls being 0.5 for an incident wave number kh nearly equal to 2.0. The triple semi-immersed Jarlan-type perforated breakwaters with significantly reduced C_R, will enhance the structure’s wave absorption ability, and lead to smaller wave forces compared with the double one. The proposed model may be used to predict the response of a structure in the preliminary design stage for practical engineering.     

Analysis of EMD algorithm for identification and extraction of an acoustic signal in underwater channel against wind driven ambient noise

Sonar generated acoustic signals transmitted in underwater channel for distant communications are affected by numerous factors like ambient noise, making them nonlinear and non-stationary in nature. In recent years, the application of Empirical Mode Decomposition （EMD） technique to analyze nonlinear and non-stationary signals has gained much attention. It is an empirical approach to decompose a signal into a set of oscillatory modes known as intrinsic mode functions （IMFs）. In general, Hilbert transform is used in EMD for the identification of oscillatory signals. In this paper anew EMD algorithm is proposed using FFT to identify and extract the acoustic signals available in the underwater channel that are corrupted due to various ambient noises over a range of 100 Hz to 10 kHz in a shallow water region.Data for analysis are collected at a depth of 5 m and 10 m offshore Chennai at the Bay of Bengal. The algorithm is validated for different sets of known and unknown reference signals. It is observed that the proposed EMD algorithmidentifies and extracts the reference signals against various ambient noises. Significant SNR improvement is alsoachieved for underwater acoustic signals.