Isotopic variation of fishes in freshwater and estuarine zones of a large subtropical coastal lagoon

We used stable C and N isotope ratios of tissues from 29 fish species from a large subtropical lagoon in southern Brazil to examine spatial variability in isotopic composition and vertical trophic structure across freshwater and estuarine habitats. Nitrogen isotope ratios indicated a smooth gradation in trophic positions among species, with most fishes occupying the secondary and tertiary consumer level. Fish assemblages showed a significant shift in their carbon isotopic signatures between freshwater and estuarine sites. Depleted carbon signatures (from −24.7‰ to −17.8‰) were found in freshwater, whereas more enriched signatures (from −19.1‰ to −12.3‰) were obtained within the estuarine zone downstream. Based on our survey of the C₃ and C₄ plants and isotopic values for phytoplankton and benthic microalgae reported for ecosystems elsewhere, we hypothesized that the observed δ¹³C differences in the fish assemblage between freshwater and estuarine sites is due to a shift from assimilating organic matter ultimately derived from C₃ freshwater marsh vegetation and phytoplankton at the freshwater site (δ¹³C ranging from −25‰ to −19‰), to C₄ salt-marsh (e.g. Spartina) and widgeon grass (Ruppia maritima), benthic microalgae and marine phytoplankton at the estuarine sites (from −18‰ to −12‰). Our results suggested that fish assemblages are generally supported by autochthonous primary production. Freshwater fishes that likely were displaced downstream into the estuary during periods of high freshwater discharge had depleted δ¹³C values that were characteristic of the upper lagoon. These results suggest that spatial foodweb subsidies can occur within the lagoon.     

Reduction of hydroelastic responses of a very-long floating structure by a floating oscillating-water-column breakwater system

The hydroelastic responses of a very-long floating structure (VLFS) placed behind a reverse T-shape freely floating breakwater with a built-in oscillating water column (OWC) chamber are analyzed in two dimensions. The Bernoulli–Euler beam equation is coupled with the equations of rigid and elastic motions of the breakwater and the VLFS. The interaction of waves between the floating rigid breakwater and the elastic VLFS is formulated in a consistent manner. It has been shown numerically that the structural deflections of the VLFS can be reduced significantly by a suitably designed reverse T-shape floating breakwater.     

Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

Numerical study of the motions and drift force of a floating OWC device

The motion and the drift force of a floating OWC (oscillating water column) wave energy device in regular waves are studied taking account of the oscillating surface-pressure due to the pressure drop across the duct of the air chamber. The potential problem inside the chamber is formulated by making use of the Green integral equation associated with the Rankine-type Green function while the outer problem with the Kelvin-type Green function. The added mass, wave damping and excitation coefficients as well as the motion and drift force of the OWC device are calculated for various values of parameter related to the pressure drop.     

Numerical simulation of undersea cable dynamics

A fully three-dimensional code has been written to compute the motion of a towed cable. The code is based on a robust and stable finite difference approximation to the differential equations derived from basic dynamics. A 3500-ft (1.07 km) cable pulled at 18.5 knots (34.3 km hr⁻¹) through a circular turn of 700 yd (0.64 km) radius has been computed in about half of the real time of the maneuver. The computed displacements are close to the measured ones; the changes in depth are within 2%.     

Carbon dioxide partial pressures in North Pacific surface waters — Time variations

Observations were made of time variations of the carbon dioxide partial pressures (Pco₂) of the atmosphere and surface sea waters in the Pacific subarctic region. Data were obtained on a cruise of the USC & GSSSURVEYOR in October, 1968 and on the TRANSPAC expedition of the CNAVENDEAVOUR in March–April, 1969. A rise in surface water Pco₂ of 18×10^–6 atm occurred in a period of 30–45 days in March–April due principally to spring warming of surface waters. An average increase of 60×10^–6 atm occurred between October, 1968 and March, 1969 as a result mainly of cessation of summer phytoplankton production and the onset of winter-storm-driven vertical mixing. Because the air-sea Pco₂ gradient not only changed appreciably in magnitude but also changed sign, there are important implications for calculations of air-sea exchange of carbon dioxide on the ocean wide scale.Data contained in this paper comprise part of a dissertation to be submitted by Louis I. Gordon in partial fulfillment of the requirements for the Ph. D. at Oregon State University.     

The estimation of acid dissociation constants in seawater media from potentionmetric titrations with strong base. I. The ionic product of water — Kw

The various assumptions implicit in the calculation of acid dissociation constants (based on ionic medium standard states) from potentiometric titrations using a cell with liquid junction (i.e. a pH measuring cell) have been examined. It was concluded that results can be obtained having an accuracy commensurate with the experimental precision. It has been shown that although the precise composition of the medium is a function of the hydrogen ion concentration (because of the protolytic nature of some of the ions in the media, e.g., sulphate and fluoride), the effect of such variations in the medium composition can be compensated for when defining the activity of hydrogen ion on an ionic medium standard state by defining the concentration of hydrogen ion as:

where β_HSO4 and β_HF are the relevant association constants and S_T and F_T are the total concentrations of sulphate and fluoride, respectively.This approach was used to obtain values for the ionic product of water (K_W) in artificial seawater media at various temperatures and ionic strengths. These were fitted to give the equation (molal concentration units):

$\text{pK}{}_{\mathrm{w}}\text{=}\text{3441.0}\text{T}\text{+2.256-0.7091}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}\text{(}\text{rms deviation 0.01}\text{)}$

where I is the formal ionic strength of the artificial seawater medium and T is the absolute temperature. The values obtained are in reasonable agreement with those found by previous workers.     

Assessment of imposex in the common whelk, Buccinum undatum (L.) from the Eastern Scheldt, The Netherlands

Several cruises were carried out on the Eastern Scheldt between September 1992 and March 1995 to study the frequency and appearance of imposex in the common whelk, Buccinum undatum L. Incidences of imposex were always > 90%. For the first time several stages of imposex in Buccinum undatum are reported. The evolution of imposex in the whelk seems to follow a similar route as in Hinia reticulata belonging to the same superfamily (Buccinacea). Tissue organotin concentrations of animals collected in February 1995 showed the highest organotin concentrations in the digestive gland and lowest in the foot of the animals. Concentrations of phenyltin compounds (up to 625 ngSn/g DW) were much higher than those of butyltin compounds (up to 40 ngSn/g DW).