Harmful microalgae blooms (HAB); problematic and conditions that induce them

HAB occurrence is becoming more frequent and problematic in marine recreational waters. However, the exploitation of the coastal area for recreational use is promoting the necessary conditions for the HAB increase. In terms of the harmful effects, we can consider two types of causative organism: the toxic producers and the high-biomass producers. Toxic events can be produced by a very low concentration of the causative organism. This characteristic implies a difficulty for the monitoring programs in relation to human health. It is important to point out in the context of human health and HAB events, that in some coastal regions (e.g. the Mediterranean basin) HABs are an emerging problem. In these regions, the local population and visitors may face a health risk that is difficult to measure. The monitoring of toxic species has mainly been associated -with shellfish farming. However, the risk of intoxication could become even greater in areas not subject to legislation of aquaculture activities.     

Effects of observed and experimental climate change on terrestrial ecosystems in northern Canada: results from the Canadian IPY program

Tundra and taiga ecosystems comprise nearly 40?% of the terrestrial landscapes of Canada. These permafrost ecosystems have supported humans for more than 4500?years, and are currently home to ca. 115,000 people, the majority of whom are First Nations, Inuit and Métis. The responses of these ecosystems to the regional warming over the past 30?C50?years were the focus of four Canadian IPY projects. Northern residents and researchers reported changes in climate and weather patterns and noted shifts in vegetation and other environmental variables. In forest-tundra areas tree growth and reproductive effort correlated with temperature, but seedling establishment was often hindered by other factors resulting in site-specific responses. Increased shrub cover has occurred in sites across the Arctic at the plot and landscape scale, and this was supported by results from experimental warming. Experimental warming increased vegetation cover and nutrient availability in most tundra soils; however, resistance to warming was also found. Soil microbial diversity in tundra was no different than in other biomes, although there were shifts in mycorrhizal diversity in warming experiments. All sites measured were sinks for carbon during the growing season, with expected seasonal and latitudinal patterns. Modeled responses of a mesic tundra system to climate change showed that the sink status will likely continue for the next 50?C100?years, after which these tundra systems will likely become a net source of carbon dioxide to the atmosphere. These IPY studies were the first comprehensive assessment of the state and change in Canadian northern terrestrial ecosystems and showed that the inherent variability in these systems is reflected in their site-specific responses to changes in climate. They also showed the importance of using local traditional knowledge and science, and provided extensive data sets, sites and researchers needed to study and manage the inevitable changes in the Canadian North.     

Polarization Models for Rayleigh Scattering Planetary Atmospheres

We present Monte Carlo simulations for the polarization of light reflected from planetary atmospheres. We investigate dependencies of intensity and polarization on three main parameters: single scattering albedo, optical depth of a scattering layer, and albedo of a Lambert surface underneath. The main scattering process considered is Rayleigh scattering, but isotropic scattering and enhanced forward scattering on haze particles are also investigated. We discuss disk integrated results for all phase angles and radial profiles of the limb polarization at opposition. These results are useful to interpret available limb polarization measurements of solar system planets and to predict the polarization of extra-solar planets as a preparation for VLT/SPHERE. Most favorable for a detection are planets with an optically thick Rayleigh-scattering layer. The limb polarization of Uranus and Neptune is especially sensitive to the vertically stratified methane mixing ratio. From limb polarization measurements constraints on the polarization at large phase angles can be set.     

Chemical and chronologic complexity in the convecting upper mantle: Evidence from the Taitao ophiolite, southern Chile

Exposure of the ca. 6 Ma Taitao ophiolite, Chile, located 50 km south of the Chile Triple Junction, allows detailed chemical and isotopic study of rocks that were recently extracted from the depleted mantle source of mid-ocean ridge basalts (DMM). Ultramafic and mafic rocks are examined for isotopic (Os, Sr, Nd, and O), and major and trace element compositions, including the highly siderophile elements (HSE). Taitao peridotites have compositions indicative of variable extents of partial melting and melt extraction. Low δ¹⁸O values for most whole rock samples suggest some open-system, high-temperature water–rock interaction, most likely during serpentinization, but relict olivine grains have δ¹⁸O values consistent with primary mantle values. Most of the peridotites analyzed for Nd–Sr isotopes have compositions consistent with estimates for the modern DMM, although several samples are characterized by ⁸⁷Sr/⁸⁶Sr and ¹⁴³Nd/¹⁴⁴Nd indicative of crustal contamination, most likely via interactions with seawater. The peridotites have initial ¹⁸⁷Os/¹⁸⁸Os ratios that range widely from 0.1168 to 0.1288 (γ_Os = −8.0 to +1.1), averaging 0.1239 (γ_Os = −2.4), which is comparable to the average for modern abyssal peridotites. A negative correlation between the Mg^# of relict olivine grains and Os isotopic compositions of whole rock peridotites suggests that the Os isotopic compositions reflect primary mantle Re/Os fractionation produced by variable extents of partial melting at approximately 1.6 Ga. Recent re-melting at or near the spatially associated Chile Ridge further modified these rocks, and Re, and minor Pt and Pd were subsequently added back into some rocks by late-stage melt–rock or fluid–rock interactions.In contrast to the peridotites, approximately half of the mafic rocks examined have whole rock δ¹⁸O values within the range of mantle compositions, and their Nd and Sr isotopic compositions are all generally within the range of modern DMM. These rocks have initial ¹⁸⁷Os/¹⁸⁸Os ratios, calculated for 6 Ma, that range from 0.126 (γ_Os = −1) to as high as 0.561 (γ_Os = +342). The Os isotopic systematics of each of these rocks may reflect derivation from mixed lithologies that include the peridotites, but may also include pyroxenites with considerably more radiogenic Os than the peridotites. This observation supports the view that suprachondritic Os present in MORB derives from mixed mantle source lithologies, accounting for some of the worldwide dichotomy in ¹⁸⁷Os/¹⁸⁸Os between MORB and abyssal peridotites.The collective results of this study suggest that this >500 km³ block of the mantle underwent at least two stages of melting. The first stage occurred at 1.6 Ga, after which the block remained isolated and unmixed within the DMM. A final stage of melting recently occurred at or near the Chile Ridge, resulting in the production of at least some of the mafic rocks. Convective stirring of this mantle domain during a >1 Ga period was remarkably inefficient, at least with regard to Os isotopes.     

Carbon and nitrogen utilization in two species of Red Sea corals along a depth gradient: Insights from stable isotope analysis of total organic material and lipids

We examined the utilization of carbon and nitrogen in two common Red Sea coral species (Stylophora pistillata and Favia favus), differing in colony morphology and polyp size, along a depth gradient down to 60 m. We describe the changes in C/N ratios and in the stable isotope composition of carbon and nitrogen of coral’s tissue and algal symbionts. We also measured the carbon isotopic composition of the lipid fraction extracted from both coral tissue and algal symbionts in order to reveal the changes in the carbon source utilized by the host coral for lipid synthesis.The results show that for both species, δ¹³C decreases by 7–8‰ in animal tissue, algal symbionts and in the lipid fractions as depth increases. However, in contrast to previous reports, the difference between δ¹³C values of coral tissue and algal symbionts does not increase with depth. δ¹⁵N values of coral tissue and algal symbionts in both species do not correlate with depth suggesting that the heterotrophic capacity of these corals does not increase with depth. δ¹³C values of tissue lipids were depleted by an average of 3.5‰ compared to δ¹³C of the entire tissue at all depths. δ¹³C values of algal lipids were depleted by an average of 2‰ compared to δ¹³C of the entire zooxanthellae at all depths, indicating high efficiency of carbon recycling between the two symbiotic partners along the entire gradient. The depletion of lipids is attributed to the fractionation mechanism during lipid synthesis. In addition, for both species, δ¹³C values of algal lipids were enriched compared with δ¹³C of tissue lipids. In S. pistillata, the difference between δ¹³C values of tissue lipids and algal lipids increased linearly with depth, indicating a change in the sources of carbon utilized by the coral for lipid synthesis below 20 m from an autotrophic to a heterotrophic source. However, in F. favus, this average difference was 4 times larger compared to shallow S. pistillata and was constant along the entire depth gradient, suggesting that F. favus uses heterotrophically-acquired carbon for lipid synthesis regardless of depth. Overall, F. favus exhibited enriched δ¹³C and δ¹⁵N values compared to S. pistillata along the entire gradient. We attribute these differences to both morphological differences (i.e. colony morphology, tissue thickness and polyp size) between the two species and to a higher heterotrophy/autotrophy ratio in F. favus at all depths. The C/N ratio in S. pistillata tissue decreased with increasing water depth whereas in F. favus it remained constant. This reflects a higher heterotrophic capacity in the large polyped F. favus, at all depths.     

Compound- and enzyme-specific phosphodiester hydrolysis mechanisms revealed by δO of dissolved inorganic phosphate: Implications for marine P cycling

We have studied the oxygen isotope signature of inorganic phosphate (P_i) generated by hydrolysis of nucleic acid phosphodiester (P-diester) compounds by cell-free enzymes (Deoxyribonuclease 1, Phosphodiesterase 1, Alkaline phosphatase) and microbial cultures at natural isotopic abundances. We demonstrate that the diesterase-catalyzed hydrolytic step leads to incorporation of at least one water O into released P_i for a total of two O atoms from water incorporated into P_i released from P-diesters. In the presence of Phosphodiesterase 1, ¹⁶O is preferentially incorporated into nucleotides released from DNA; whereas ¹⁸O is preferentially incorporated into nucleotides released from RNA. A strong consistency between predicted O-isotope regeneration signatures based on results of cell-free enzyme experiments and measured isotopic signatures from independent experiments with E. coli cultures was observed and confirms proposed models for phosphoester hydrolysis. Results from these studies made at natural ¹⁸O abundance levels provide a new tool, enzyme-specific O-isotope fractionation, for investigations of organophosphate metabolism and phosphorus cycling pathways in natural aquatic systems.     

Babbling brook to thunderous torrent: Using sound to monitor river stage

The passive, ambient sound above the water from a river has previously untapped potential for determining flow characteristics such as stage. Measuring sub-aerial sound could provide a new, efficient way to continuously monitor river stage, without the need for in-stream infrastructure. Previous published work has suggested that there might be a relationship between sound and river stage, but the analysis has been restricted to a narrow range of flow conditions and river morphologies. We present a method to determine site suitability and the process of how to record and analyse sound. Data collected along a 500 m length of the River Washburn during July 2019 is used to determine what makes a site suitable for sound monitoring. We found that sound is controlled by roughness elements in the channel, such as a boulder or weir, which influences the sound produced. On the basis of these findings, we collect audio recordings from six sites around the northeast of England, covering a range of flow conditions and different roughness elements, since 2019. We use data from those sites collected during storms Ciara and Dennis to produce a relationship between this sound and river stage. Our analysis has shown a positive relationship between an R² of 0.73 and 0.99 in all rivers, but requires careful site selection and data processing to achieve the best results. We introduce a filter that is capable of isolating a river's sound from other environmental sound. Future work in examining the role of these roughness elements is required to understand the full extent of this technique. By demonstrating that sound can operate as a hydrometric tool, we suggest that sound monitoring could be used to provide cost-effective monitoring devices, either to detect relative change in a river or, after more research, a reliable stage measurement.     

Brine evolution in two inland evaporative environments: influence of microbial mats in mineral precipitation

This paper gives new insight into the precipitation sequences in six playa basins that host microbial mats. The study basins are distributed across two evaporitic endorheic drainage systems located in the Central part of Spain with markedly different hydrochemistry and mineralogy. One group, in the north, consists of highly alkaline, brackish to saline lakes containing a high concentration of chloride with dominant carbonate over sulphates. A second group of lakes are mesosaline to hypersaline, with sulphate the dominant anion over chloride. Mineral assemblages identified in both contain several phases that provide evidence for mixed carbonate-sulphate precipitation pathways, in the north, and sulphate-dominated pathways in the south. Regardless of their ionic composition, saline lakes support thin veneers of microbial mats which, by integrating several lines of evidence (hydrochemical and physical analyses, statistical analyses of ions, mineralogical assemblages, textural relationships among mineral phases and microbial mats) are shown to modify the chemical behavior of the evaporitic sediment and promote the formation of carbonates and sulphates from Ca-poor waters with high Mg/Ca ratios. Geochemical changes induced in the environment surrounding the microorganism favor the nucleation of hydrated Mg-carbonates (hydromagnesite and nesquehonite), calcite and dolomite. Simultaneously, the microbial mats provide nucleation sites for gypsum crystals, where they are subjected to episodic stages of growth and dissolution due to saturation indices close to zero. In addition, the bubbles produced by the metabolic activities of microorganisms are shown to promote the precipitation of hydrated Mg-sulphates, despite permanent subsaturation levels. Although common in the studied playa basins, this effect has not been previously reported and is key to understanding sulphate behavior and distribution. Modern and natural evaporitic microbial environments are important analogs for understanding brine evolution and mineral precipitation pathways in shallow water settings that have existed since the Archean on Earth and perhaps on Mars.