Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Experimental impact cratering: A summary of the major results of the MEMIN research unit

This paper reviews major findings of the Multidisciplinary Experimental and Modeling Impact Crater Research Network (MEMIN). MEMIN is a consortium, funded from 2009 till 2017 by the German Research Foundation, and is aimed at investigating impact cratering processes by experimental and modeling approaches. The vision of this network has been to comprehensively quantify impact processes by conducting a strictly controlled experimental campaign at the laboratory scale, together with a multidisciplinary analytical approach. Central to MEMIN has been the use of powerful two-stage light-gas accelerators capable of producing impact craters in the decimeter size range in solid rocks that allowed detailed spatial analyses of petrophysical, structural, and geochemical changes in target rocks and ejecta. In addition, explosive setups, membrane-driven diamond anvil cells, as well as laser irradiation and split Hopkinson pressure bar technologies have been used to study the response of minerals and rocks to shock and dynamic loading as well as high-temperature conditions. We used Seeberger sandstone, Taunus quartzite, Carrara marble, and Weibern tuff as major target rock types. In concert with the experiments we conducted mesoscale numerical simulations of shock wave propagation in heterogeneous rocks resolving the complex response of grains and pores to compressive, shear, and tensile loading and macroscale modeling of crater formation and fracturing. Major results comprise (1) projectile–target interaction, (2) various aspects of shock metamorphism with special focus on low shock pressures and effects of target porosity and water saturation, (3) crater morphologies and cratering efficiencies in various nonporous and porous lithologies, (4) in situ target damage, (5) ejecta dynamics, and (6) geophysical survey of experimental craters.     

Current and thermohaline characteristics of the Arabian Sea during January 1998

Based on a ship survey during January 1998, the characteristics of the flow, the thermohaline properties and the volume transport of the Arabian Sea are discussed. A strong westward flow exists between 10.5?N and 11?N, part of which turns to the south as the Somali current near the coast at about 10?N and the rest turns north. At the passage between the African continent and the So- cotra Island, the northern branch separates into two flows: the left one enters the passage and the right one flows eastward along the southern slope of the island. Off the island the flow separates once more, most of it meandering northeast and a small fraction flow- ing southeast. Volume transport calculation suggests that the tidal transport is one or two orders of magnitude smaller than the total transport in this region and it becomes more important near the coast. The average velocity of the flow in the upper layer (0-150 m) is about 20 cm s-1, with a maximum of 53 cm s-1 appearing east of the Socotra Island, and the subsurface layer (200-800 m) has an aver- age velocity of 8.6 cm s-1; the velocity becomes smaller at greater depths. The depth of the seasonal thermocline is about 100 m, above which there is a layer with well mixed temperature and dissolved oxygen. High-salinity and oxygen-rich water appears near the surface of the northern Arabian Sea; a salinity maximum and oxygen minimum at 100 m depth along 8?N testifies the subduction of surface water from the northern Arabian Sea. Waters from the Red Sea and the Persian Gulf also influence the salinity of the area.     

Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa

1　Introduction　Generallyknownasacodominantgeneticmarker ,microsatellitehasbeenwidelyusedinstudiesonpopu lationgenetics,high resolutiongenotyping ,genemap ping ,evolution ,linkageanalysis ,conservationbiology ,behaviouralecology ,relationsbetweenparasite…     

VARIATIONAL ITERATION SOLVING METHOD FOR SEA-AIR OSCILLATOR MODEL OF INTERDECADAL CLIMATE FLUCTUATIONS

1IN T R O D U C T IO NInteractionsbetween the ocean and atmosphere con-tributeto climatefluctuationosver a broad spectrum oftime scales.Studiesof those interactionhsave farfo-cused on El Ni o-SouthernOscillation(ENSO)phe-nomenon thathas a period of3to4yea…     

A search for periodicities from a ULX in the LINER galaxy NGC 4736

We report our findings on a new quasi-periodic oscillation (QPO) and a long period from the ultraluminous X-ray source (ULX) X-2 in nearby galaxy NGC 4736 based on the Chandra and XMM-Newton archival data. To examine the timing properties, power density spectra of the source have been obtained using Fast Fourier Transform. Also the spectral parameters of the source have been calculated by obtaining and fitting the energy spectra. Power density spectrum of this source reveals a QPO peak at \(0.73_{-0.14}^{+0.16}~\mathrm{mHz}\) with an fractional rms variability of 16 % using the Chandra data (in the year 2000-lower state of the source). The XMM-Newton data analysis indicates a peak at \(0.53_{-0.35}^{+0.09}~\mathrm{mHz}\) with a fractional rms variation of 5 % (in the year 2006-higher state of the source). These recovered QPOs overlap within errors and may be the same oscillation. In addition, we detect a long periodicity or a QPO in the Chandra data of about (5.2±2.0)×10^?5 Hz (～5.4 h) over 3 σ confidence level. If this is a QPO, it is the lowest QPO detected from a ULX. The mass of the compact object in ULX X-2 is estimated using the Eddington luminosity and a disk blackbody model in the range (10–80) M _⊙.     

A proposal for the theoretical analysis of the interactive coupled effects between urbanization and the eco-environment in mega-urban agglomerations

Mega-urban agglomerations are strategic core areas for national economic development and the main regions of new urbanization. They also have important roles in shifting the global economic center of gravity to China. However, the development of mega-urban agglomerations has triggered the interactive coercion between resources and the eco-environment. The interactive coupled effects between urbanization and the eco-environment in mega-urban agglomerations represent frontier and high-priority research topics in the field of Earth system science over the next decade. In this paper, we carried out systematic theoretical analysis of the interactive coupling mechanisms and coercing effects between urbanization and the eco-environment in mega-urban agglomerations. In detail, we analyzed the nonlinear-coupled relationships and the coupling characteristics between natural and human elements in mega-urban agglomerations. We also investigated the interactive coercion intensities between internal and external elements, and the mechanisms and patterns of local couplings and telecouplings in mega-urban agglomeration systems, which are affected by key internal and external control elements. In addition, we proposed the interactive coupling theory on urbanization and the eco-environment in mega-urban agglomerations. Furthermore, we established a spatiotemporal dynamic coupling model with multi-element, multi-scale, multi-scenario, multi-module and multi-agent integrations, which can be used to develop an intelligent decision support system for sustainable development of mega-urban agglomerations. In general, our research may provide theoretical guidance and method support to solve problems related to mega-urban agglomerations and maintain their sustainable development.     

Ammonium Silicate Stability Relations

A method has been developed to control ammonium fugacity, \(f_{{\text{NH}}_{3}}\), at elevated temperatures and pressures. The method uses an internal nitrogen buffer, the assemblage Cr + CrN, in conjunction with a traditional external hydrogen buffer. In this manner, all gas fugacities in the system N-O-H can be calculated.The Cr + CrN buffer has been applied to study equilibria between buddingtonite (ammonium feldspar), ammonium muscovite, sillimanite, and quartz at a constant gas pressure of 2,000 bars. Two of the five relevant reactions were measured experimentally; from these data, it is possible to calculate isothermal sections at 500, 600, and 700° C.Below 600° C, ammonium muscovite is stable even at extremely low levels of \(f_{{\text{NH}}_{3}}\), while buddingtonite requires \(f_{{\text{NH}}_{3}}\;\geqq\;10^4\) bars. Release of NH₃ during progressive metamorphism can be achieved by three processes: thermal decomposition, dehydration, and cation exchange. Within the crust, \(f_{{\text{NH}}_{3}}\) predominates over \(f_{{\text{N}}_{2}}\) by several orders of magnitude; but on the surface, nitrogen released as NH₃ by metamorphism will be oxidized to N₂. Biological materials provide important intermediate storage for nitrogen compounds during the nitrogen cycle.