Sensitivity of bacterioplankton nitrogen metabolism to eutrophication in sub-tropical coastal waters of Key West, Florida

Expression of intracellular ammonium assimilation enzymes were used to assess the response of nitrogen (N) metabolism in bacterioplankton to N-loading of sub-tropical coastal waters of Key West, Florida. Specific activities of glutamine synthetase (GS) and total glutamate dehydrogenase (GDH_T) were measured on the bacterial size fraction (<0.8 μm) to assess N-deplete versus N-replete metabolic states, respectively. Enzyme results were compared to concentrations of dissolved organic matter and nutrients and to the biomass and production of phytoplankton and bacteria. Concentrations of dissolved inorganic N (DIN), dissolved organic N (DON), and dissolved organic carbon (DOC) positively correlated with specific activities of GDH_T and negatively correlated with that of GS. Total dissolved N (TDN) concentration explained 81% of variance in bacterioplankton GDH_T:GS activity ratio. The GDH_T:GS ratio, TDN, DOC, and bacterial parameters decreased in magnitude along a tidally dynamic trophic gradient from north of Key West to south at the reef tract, which is consistent with the combined effects of localized coastal eutrophication and tidal exchange of seawater from the Southwest Florida Shelf and Florida Strait. The N-replete bacterioplankton north of Key West can regenerate ammonium which sustains primary production transported south to the reef. The range in GDH_T:GS ratios was 5–30 times greater than that for commonly used indicators of planktonic eutrophication, which emphasizes the sensitivity of bacterioplankton N-metabolism to changes in N-bioavailability caused by nutrient pollution in sub-tropical coastal waters and utility of GDH_T:GS ratio as an bioindicator of N-replete conditions.     

Application of biomarkers for improving risk assessments of chemicals under the Water Framework Directive: a case study

To answer the requirement of the European Commission's Water Framework Directive (WFD) for biological-effects endpoints to classify the ecological health of aquatic ecosystems, we propose the biomarker response index (BRI). The BRI, based on a suite of biomarkers at different levels of biological response at the individual level, provides an integrated relative measure of the general health status of coastal invertebrates. Using the BRI, the health of mussels (Mytilus edulis) from 10 estuaries classified by the Environment Agency of England and Wales under the WFD was compared. Eight sites were healthier than predicted and two showed a similar health status to that of the predicted point-source pollution risk classification. Results indicate that the BRI offers a potential measure of organism health that can be used in monitoring under the WFD as an additional aid to reduce uncertainty in defining risk classification and to provide better evidence of existing impact.     

Overview of integrative tools and methods in assessing ecological integrity in estuarine and coastal systems worldwide

In recent years, several sets of legislation worldwide (Oceans Act in USA, Australia or Canada; Water Framework Directive or Marine Strategy in Europe, National Water Act in South Africa, etc.) have been developed in order to address ecological quality or integrity, within estuarine and coastal systems. Most such legislation seeks to define quality in an integrative way, by using several biological elements, together with physico-chemical and pollution elements. Such an approach allows assessment of ecological status at the ecosystem level ('ecosystem approach' or 'holistic approach' methodologies), rather than at species level (e.g. mussel biomonitoring or Mussel Watch) or just at chemical level (i.e. quality objectives) alone. Increasing attention has been paid to the development of tools for different physico-chemical or biological (phytoplankton, zooplankton, benthos, algae, phanerogams, fishes) elements of the ecosystems. However, few methodologies integrate all the elements into a single evaluation of a water body. The need for such integrative tools to assess ecosystem quality is very important, both from a scientific and stakeholder point of view. Politicians and managers need information from simple and pragmatic, but scientifically sound methodologies, in order to show to society the evolution of a zone (estuary, coastal area, etc.), taking into account human pressures or recovery processes. These approaches include: (i) multidisciplinarity, inherent in the teams involved in their implementation; (ii) integration of biotic and abiotic factors; (iii) accurate and validated methods in determining ecological integrity; and (iv) adequate indicators to follow the evolution of the monitored ecosystems. While some countries increasingly use the establishment of marine parks to conserve marine biodiversity and ecological integrity, there is awareness (e.g. in Australia) that conservation and management of marine ecosystems cannot be restricted to Marine Protected Areas but must include areas outside such reserves. This contribution reviews the current situation of integrative ecological assessment worldwide, by presenting several examples from each of the continents: Africa, Asia, Australia, Europe and North America.     

Role of a rheophyte in bench development on a sand‐bed river in southeast Australia

Casuarina cunninghamiana Miq. is an important rheophytic tree in New South Wales, Australia because it is fast growing and can tolerate flood disturbance. Widden Brook is an active sand‐bed stream that has widened substantially since initial European settlement in the early 1800s and is characterized by high flood variability and multi‐decadal periods of alternating high and low flood frequency, called flood‐ and drought‐dominated regimes. Channel contraction by bench formation is currently occurring. Conversion of coarse‐grained point bars to benches is an important process of channel contraction. When point bars grow to a height where suspended sediment is first deposited to thicknesses of at least 50 mm by sub‐bankfull floods, rapid establishment of C. cunninghamiana occurs. As the trees grow they partially block bankside flows, thereby locally reducing flow velocity and inducing further deposition on the benches. Such synergistic relationships between bar height and inundation, fine‐grained sediment deposition, tree establishment and the development of a bankside low current velocity zone are fundamental for bench development. Size‐class frequency data demonstrate that C. cunninghamiana on the benches consists of pure even‐aged stands with most trees clustering near the average diameter. Two benches have similar size class frequency distributions but a third has significantly smaller trees. Recruitment on benches is episodic, may occur in areas open to grazing and is dependent on favourable conditions that allow tree survival. These favourable conditions include high seed availability, low levels of competition, deposition of fine sediments and adequate moisture for tree growth. Although C. cunninghamiana germinates on bars, seedlings are eliminated by prolonged inundation or flood scour and do not reach maturity. Recurring catastrophic floods or a sequence of large floods in rapid succession episodically destroy benches by substantial channel widening and initiate a new phase of bar and bench development. A conceptual model of the conversion of point bars to benches by thick mud deposition and C. cunninghamiana recruitment has been developed for sand‐bed streams draining similar sandstone catchments. Copyright © 2009 John Wiley & Sons, Ltd.     

Quantifying sediment storage in a high alpine valley (Turtmanntal,Switzerland)

The determination of sediment storage is a critical parameter in sediment budget analyses. But, in many sediment budget studies the quantification of magnitude and time‐scale of sediment storage is still the weakest part and often relies on crude estimations only, especially in large drainage basins (>100 km²). We present a new approach to storage quantification in a meso‐scale alpine catchment of the Swiss Alps (Turtmann Valley, 110 km²). The quantification of depositional volumes was performed by combining geophysical surveys and geographic information system (GIS) modelling techniques. Mean thickness values of each landform type calculated from these data was used to estimate the sediment volume in the hanging valleys and the trough slopes. Sediment volume of the remaining subsystems was determined by modelling an assumed parabolic bedrock surface using digital elevation model (DEM) data. A total sediment volume of 781·3×10⁶–1005·7×10⁶ m³ is deposited in the Turtmann Valley. Over 60% of this volume is stored in the 13 hanging valleys. Moraine landforms contain over 60% of the deposits in the hanging valleys followed by sediment stored on slopes (20%) and rock glaciers (15%). For the first time, a detailed quantification of different storage types was achieved in a catchment of this size. Sediment volumes have been used to calculate mean denudation rates for the different processes ranging from 0·1 to 2·6 mm/a based on a time span of 10 ka. As the quantification approach includes a number of assumptions and various sources of error the values given represent the order of magnitude of sediment storage that has to be expected in a catchment of this size. Copyright © 2009 John Wiley & Sons, Ltd.     

The relationship of Irgarol and its major metabolite to resident phytoplankton communities in a Maryland marina, river and reference area

The objectives of this study were to: (1) measure water column concentrations of Irgarol 1051 and its major metabolite GS26575 annually (2004-2006) during mid-June and mid-August at 14 sites in a study area comprised of three sub-regions chosen to reflect a gradient in Irgarol exposure (Port Annapolis marina, Severn River and Severn River reference area); (2) use a probabilistic approach to determine ecological risk of Irgarol and its major metabolite in the study area by comparing the distribution of exposure data with toxicity-effects endpoints; and (3) measure both functional and structural resident phytoplankton parameters concurrently with Irgarol and metabolite concentrations to assess relationships and determine ecological risk at six selected sites in the three study areas described above. The three-year summer mean Irgarol concentrations by site clearly showed a gradient in concentrations with greater values in Back Creek (400-500 ng/L range), lower values in the Severn River sites near the confluence with Back Creek (generally values less than 100 ng/L) and still lower values (<10 ng/L) at the Severn River reference sites at the confluence with Chesapeake Bay. A similar spatial trend, but with much lower concentrations, was also reported for GS26575. The probability of exceeding the Irgarol plant 10th centile of 193 ng/L and the microcosm NOEC (323 ng/L) suggested high ecological risk from Irgarol exposure at Port Annapolis marina sites but much lower risk at the other sites. There were no statistically significant differences among the three site types (marina, river and reference) with all years combined or among years within a site type for the following functional and structural phytoplankton endpoints: algal biomass, gross photosynthesis, biomass normalized photosynthesis, chlorophyll a, chlorophyll a normalized photosynthesis and taxa richness. Therefore, based on the above results, Irgarol adverse effects predicted from the plant 10th centile and the microcosm NOEC in the high Irgarol exposure area (Back Creek/Port Annapolis marina) were not confirmed with the actual field data for the receptor species (phytoplankton). These results also highlight the importance of unconfined field studies with a chemical gradient in providing valuable information regarding the responses of resident phytoplankton to herbicides.     

Dipping-interface Mapping Using Mode-separated Rayleigh Waves

Multichannel analysis of surface waves (MASW) method is a non-invasive geophysical technique that uses the dispersive characteristic of Rayleigh waves to estimate a vertical shear (S)-wave velocity profile. A pseudo-2D S-wave velocity section is constructed by aligning 1D S-wave velocity profiles at the midpoint of each receiver spread that are contoured using a spatial interpolation scheme. The horizontal resolution of the section is therefore most influenced by the receiver spread length and the source interval. Based on the assumption that a dipping-layer model can be regarded as stepped flat layers, high-resolution linear Radon transform (LRT) has been proposed to image Rayleigh-wave dispersive energy and separate modes of Rayleigh waves from a multichannel record. With the mode-separation technique, therefore, a dispersion curve that possesses satisfactory accuracy can be calculated using a pair of consecutive traces within a mode-separated shot gather. In this study, using synthetic models containing a dipping layer with a slope of 5, 10, 15, 20, or 30 degrees and a real-world example, we assess the ability of using high-resolution LRT to image and separate fundamental-mode Rayleigh waves from raw surface-wave data and accuracy of dispersion curves generated by a pair of consecutive traces within a mode-separated shot gather. Results of synthetic and real-world examples demonstrate that a dipping interface with a slope smaller than 15 degrees can be successfully mapped by separated fundamental waves using high-resolution LRT.     

Kinematic transformations for planar multi‐directional pseudodynamic testing

The pseudodynamic (PSD) test method imposes command displacements to a test structure for a given time step. The measured restoring forces and displaced position achieved in the test structure are then used to integrate the equations of motion to determine the command displacements for the next time step. Multi‐directional displacements of the test structure can introduce error in the measured restoring forces and displaced position. The subsequently determined command displacements will not be correct unless the effects of the multi‐directional displacements are considered. This paper presents two approaches for correcting kinematic errors in planar multi‐directional PSD testing, where the test structure is loaded through a rigid loading block. The first approach, referred to as the incremental kinematic transformation method, employs linear displacement transformations within each time step. The second method, referred to as the total kinematic transformation method, is based on accurate nonlinear displacement transformations. Using three displacement sensors and the trigonometric law of cosines, this second method enables the simultaneous nonlinear equations that express the motion of the loading block to be solved without using iteration. The formulation and example applications for each method are given. Results from numerical simulations and laboratory experiments show that the total transformation method maintains accuracy, while the incremental transformation method may accumulate error if the incremental rotation of the loading block is not small over the time step. A procedure for estimating the incremental error in the incremental kinematic transformation method is presented as a means to predict and possibly control the error. Copyright © 2009 John Wiley & Sons, Ltd.