Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

Multiple Unmanned Underwater Vehicles Consensus Control with Unmeasurable Velocity Information and Environmental Disturbances Under Switching Directed Topologies

China Ocean Engineering - A consensus algorithm proposed in the paper is applied to tackle remarkable problems of unmeasurable velocities, the environmental disturbances, and the limited...     

基于功能视角的广州都市边缘区乡村发展类型分化及其动力机制

基于村庄规划详细调查数据库,采用空间集中度和功能识别等方法,深度剖析广州都市边缘区村域尺度乡村发展的类型分化及其动力机制.结果 表明:①广州都市边缘区村域乡村功能的空间梯度差异特征明显.乡村经济发展功能和社会保障功能呈现出由城区向城郊逐渐减弱的分异特征,农业生产功能和生态保育功能则由城区向城郊逐渐增强态势.以乡村主导功...     

Perceptions of Land Tenure Security in Rural China: The Impact of Land Reallocations and Certification

Abstract

Tenure security is commonly recognized as an important factor in stimulating long-term investments in land. Recent studies suggest that a distinction between legal, actual and perceived tenure security needs to be made in analyzing tenure security. This study discusses the relationships between legal, actual and perceived land tenure security in rural China, and empirically examines the impact of actual on perceived land tenure security by applying Probit models to household and village survey data collected in four provinces. Using household expectations about the absence of land reallocations within the next five years as the dependent variable, we find that tenure security is positively affected by the possession of land certificates in villages that periodically reallocated land but not in villages that did not do so. The estimated impact is larger for land certificates issued in the new round of land certification than for land certificates that were issued earlier.     

耀变体在射电波段的偏振研究

利用UMRAO数据库22个耀变体源的偏振数据,根据Lazarian&Pogosyan理论模型分析研究了耀变体射电波段的偏振随波长的变化,得出不同类的耀变体偏振随波长变化的原因。结果表明,与Lazarian&Pogosyan理论模型吻合较好。由此得出如下结论:(1)反常去偏振耀变体源中反常法拉第旋光起主导作用,反常去偏振相对较少;(2)热辐射与同步辐射混合作用,高频波段的偏振度小于低频波段的偏振度(反常去偏振);(3)当被观测波段波长小于吸积盘热辐射对应的维恩波长时,高频波段的偏振度大于低频波段的偏振度(常规去偏振)。     

Rural settlement spatial patterns and effects: Road traffic accessibility and geographic factors in Guangdong Province,China

Journal of Geographical Sciences - Based on land use classification data of remote sensing images, using kernel density, the minimal cumulative resistance model of road traffic accessibility, and a...     

Rift structures and its related unconformities on and adjacent the Dongsha Rise: insights into the nature of the high-velocity layer in the northern South China Sea

Marine Geophysical Research - It is curious that the typical feature of high-velocity layer (HVL) underneath the crust for the magma-rich margin was found in the magma-poor margin of the South...     

多向不规则波浪作用下群墩结构上爬高的计算研究

波浪的方向分布对波浪的传播及其与工程结构物的作用都具有明显影响,目前现有的研究大多是基于单向波浪进行的。为了研究方向分布对群墩结构上的爬高影响,基于规则波浪与群墩作用的理论解,结合多向不规则波浪的造波方法,建立了多向不规则波浪与群墩作用的计算模型,同时进行了物理模型试验对模型的有效性进行了验证。系统地对群墩周围及表面上的波浪爬高进行了计算分析,结果表明,方向分布对波浪爬高具有较大的影响,且不同位置处的影响并不相同,在实际的工程设计中如果按照单向波浪计算,可能低估或者高估群墩周围的爬高。     

海州湾4种鱼类生长特征的空间异质性

传统的渔业资源评估均假设鱼类的生长参数是匀质的,然而近年来越来越多的研究表明海洋鱼类生长存在空间异质性。为探究海州湾鱼类生长参数的空间异质性现象,本研究分析了2013–2018年海州湾及其邻近海域方氏云鳚(Pholis fangi)、尖海龙(Syngnatus acus)、小黄鱼(Larimichthys polyactis)和赤鼻棱鳀(Thryssa kammalensis)的空间分布,使用电子体长频率分析方法结合Bootstrap重抽样方法估算了这4种鱼类的生长参数及其在深、浅水区域中的差异。结果显示,这4种鱼类生长参数均表现出一定的空间异质性,其中尖海龙和小黄鱼生长参数的空间异质性表现较为明显。这种差异可能是由于空间上的理化条件、群落结构以及物种本身洄游分布的差异而产生的。     

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.