温度和盐度对半滑舌鳎幼鱼消化酶活性的影响

以半滑舌鳎(Cynoglossus semilaevis Günther)幼鱼为实验对象,分别设置3个盐度水平(22、26和30)和4个温度水平(18,21,24和27 ℃),采用酶活性分析的方法比较研究了在不同温度和盐度的养殖环境下,半滑舌鳎肝胰脏和肠道内不同部位蛋白酶、淀粉酶和脂肪酶3种消化酶活性的差异.结果表明,盐度和温度对半滑舌鳎消化酶活性影响均显著 (P<0.05).不同温度和盐度组合下,3种消化酶活性变化有所差异.总体比较,肝胰脏和肠道内3种消化酶比活力均以盐度26、温度21和24 ℃组合下活性较高.在相同温度和盐度下,肝胰脏和肠道的不同部位的3种消化酶活性有所差异,其中,蛋白酶比活力以肝胰脏和后肠最高,显著高于前肠和中肠(P<0.05);淀粉酶比活力以肝胰脏内最高,各肠段之间淀粉酶比活力差异不显著(P>0.05);淀粉酶比活力也以肝胰脏内最高,其次分别为前肠、后肠和中肠,各部位脂肪酶比活力差异显著(P>0.05).本研究发现半滑舌鳎幼鱼体内淀粉酶比活力相对比较高,说明半滑舌鳎幼鱼对淀粉具有较强的消化能力,建议在配制半滑舌鳎幼鱼饲料时可适当提高淀粉比例,以降低饲料成本.     

Identification of a Putative Tetrasporophyte-Specific Gene in Gracilaria lemaneiformis(Gracilariales, Rhodophyte)

A putative tetrasporophyte-specific gene, designated as SSH466 (GenBank accession No. DQ019223), was one of the genes identified in this work using suppression subtractive hybridization (SSH) method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides, including an open reading frame of 498 nucleotides encoding 166 amino acid residues, 158 nucleotides of 5' untranslated region and 363 nucleo- tides of 3' non-coding region. Protein motif and secondary structure prediction showed that there existed a transmembrane domain with a unique β-sheet. Thus, SSH466 protein might be a cross-membrane protein. Sequence homology search in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed that it was a tetrasporo- phyte-specific gene.     

3DGIS在公路设计工程中的应用

为了优化公路设计方法,探索了3DGIS技术在公路设计工程方面的应用。从公路设计中的道路选线优化、新设计道路的三维可视化以及土石方工程量的计算等几个方面研究了数字高程模型（DEM）与ArcGIS的三维分析模块在公路设计中的应用。再现了带状数字地面模型,为道路的平面选线工作提供了准确快速的坡度、坡向分析方法,通过行车模拟对道路设计效果进行评价。3DGIS技术辅助公路设计,使得公路设计方案更为直观、科学。     

基于ArcGIS Server的赣州市旅游信息系统设计

主要论述了赣州市旅游信息系统的总体设计原则、功能模块设计及其开发思路,简要介绍了系统构建所需的软硬件平台和关键技术,以及在.NET平台下利用ArcGIS Server来构建该系统的W ebGIS应用程序。     

Organic Carbon in the Surface Sediments from the Intensive Mariculture Zone of Sanggou Bay: Distribution,Seasonal Variations and Sources

Mariculture has rapidly grown worldwide, which might greatly influence the biogeochemical cycle of organic carbon in coastal seas. In this study, several geochemical parameters, including grain size composition, sedimentary total organic carbon(TOC), total nitrogen(TN), stable carbon(δ13 C) and nitrogen(δ15 N) isotopic compositions, were analyzed for surface sediments collected from different mariculture zones of Sanggou Bay and in different seasons. We investigated the composition and distribution of organic matter in surface sediments and further evaluated the contribution of mariculture activities to TOC sources. The TOC and TN contents(mass percentage) in the bay were in the range of 0.14% to 1.45% and 0.03% to 0.20%, respectively. The spatial distribution indicated that sedimentary TOC and TN contents in shellfish monoculture and shellfish-kelp polyculture zones were higher than in other mariculture zones, which might be related to grain size composition and mariculture organisms. Seasonal variations of TOC contents were observed in different mariculture zones. The TOC/TN atomic ratio(C/N), δ13 C and δ15 N were in the ranges of 5.97 to 10.97,-21.76‰ to-13.14‰ and 2.13‰ to 8.08‰, respectively, implying that sedimentary organic matter in Sanggou Bay was the mixture of marine phytoplankton, terrestrial and maricultural sources. A simple mixing model based on δ13 C was applied and the results indicated that the relative contributions of organic carbon sources in Sanggou Bay followed the order kelp(36.6%) ? marine phytoplankton(28.7%) ? shellfish bio-deposition(23.8%) ? terrestrial input(10.9%). Surface sediments in Sanggou Bay were dominated by mariculture-derived organic carbon, which on average accounted for 60.4% of TOC.     

地震后水汽异常变化初探

研究东日本地震、汶川地震和玉树地震震中及其附近区域在地震前后的水汽时间序列变化。首先分析震中MODIS水汽序列和震中附近探空站点水汽序列在地震前后的变化;然后基于GNSS ZTD与水汽之间的高相关性,以GNSS ZTD代替GNSS水汽,讨论震源区周围IGS站点的ZTD序列变化。研究发现,震后震中及其附近区域水汽值变化出现异常,且距离越近所受影响越大;水汽不断聚积,达到峰值后发生降水。     

胶莱盆地东缘早白垩世莱阳群沉积序列及岩相古地理

胶莱盆地东缘早白垩世早期莱阳群沉积体现了湖盆由开始形成—鼎盛—萎缩消亡的全过程,发育由冲洪积相—湖相—三角洲相—河流相沉积的完整序列,且由东至西,由湖相到河流相逐渐过渡;古水流方向显示莱阳期盆地中心位于朱吴断裂、海阳断裂带之间,以断裂带为界,以西水流方向大致为由NW至SE向,以东水流方向则为由SE至NW向;早白垩世早期莱阳期盆地的形成与演化明显受区域性牟(平)-即(墨)断裂带活动所控制。     

杭州湾及其邻近海域表层沉积物的沉积环境分区及重金属污染特征分析

于2017年春季和2018年春季,分两个航次,在杭州湾及其邻近海域采集了表层沉积物样品,并对其粒度组成及重金属含量进行了测定。结果表明,研究区域表层沉积物的粒级组成以砂和粉砂为主,平均占比分别为38.0%和55.4%。根据粒级组成的区域分布特征,将研究区域分为长江河口现代沉积区(Ⅰ-1)、钱塘江河口现代沉积区(Ⅰ-2)、长江羽状锋面积聚区(Ⅱ-1)、长江次级锋面积聚区(Ⅱ-2)、岛屿风化影响区(Ⅲ)、残留砂混合沉积区(Ⅳ)等4大类6个亚区。通过比较不同区域重金属含量发现,总体分布趋势为Ⅱ-1区和Ⅱ-2区含量较高,Ⅰ-2区和Ⅳ区含量较低,Ⅰ-1区和Ⅳ区相对居中。单因子污染指数评价结果显示,Ⅱ-1区和Ⅱ-2区的Cu、Pb、Zn、As、Cd、Hg已呈现出污染态势,需引起关注;Cr则未呈现出污染态势。     

LED光色对欧洲舌齿鲈幼鱼抗氧化能力和消化能力的影响

作者利用循环水养殖实验系统,研究了红、绿、白、黄、蓝5种光色对欧洲舌齿鲈(Dicentrarchus labrax)幼鱼抗氧化能力和消化能力的影响。结果表明,红光组的欧洲舌齿鲈幼鱼超氧化物歧化酶(SOD)活力显著高于其他光色组(P0.05),而绿、白、黄和蓝光色组的SOD活力没有显著性差异;红光组的还原型谷胱甘肽(GSH)含量显著高于其他光色组(P0.05),绿、白光组与黄、蓝光组的GSH含量之间有显著性差异(P0.05);红光组过氧化氢酶(CAT)活力显著高于其他光色组(P0.05),白光组的CAT活力显著低于其他光色组(P0.05)。黄光组的胃蛋白酶活力显著高于红、绿、白光组(P0.05),绿光组的胃蛋白酶活力显著低于其他光色组(P0.05);红、绿、蓝光组的淀粉酶活力要显著高于白、黄光组(P0.05),红、绿、蓝光组的淀粉酶活力没有显著性差异,黄、白光组的淀粉酶活力没有显著性差异;白光组纤维素酶活力显著高于其他光色组(P0.05),绿光组的纤维素酶活力显著高于红、蓝光组(P0.05),黄光组的纤维素酶活力和绿、红、蓝光组没有显著性差异。因此,欧洲舌齿鲈在红光下养殖,其体内的抗氧化能力强,能有效应对氧化应激,而在黄光或红光下养殖其消化能力更强。