Distributions of dissolved and particulate biogenic thiols in the subartic Pacific Ocean

Dissolved and particulate concentrations of the biogenic thiols cysteine (Cys), arginine–cysteine (Arg–Cys), glutamine–cysteine (Gln–Cys), γ-glutamate–cysteine (γ-Glu–Cys) and glutathione (GSH) were measured in the subartic Pacific Ocean in the summer of 2003 using high performance liquid chromatography (HPLC) with precolumn derivatization as reported in previous work. In this study, a preconcentration protocol for the derivatized thiols was utilized to extend detection limits of dissolved thiols to picomolar levels. The measured concentrations of particulate and dissolved thiols were uncoupled, with distinctive depth profiles and large differences in the particulate to dissolved ratios between individual compounds. Glutathione was the most abundant particulate thiol whereas the most abundant dissolved thiol was γ-Glu–Cys, with concentrations as high as 15 nM. Given the relatively small pool of intracellular γ-Glu–Cys and the very low dissolved concentrations of GSH, we hypothesize that glutathione released from cells is rapidly converted to the potentially degradation resistant γ-Glu–Cys outside the cell. The relatively high concentrations of other dissolved thiols compared to particulate concentrations implies both biological exudation and slow degradation rates. Some thiols appear to vary with changes in nutrient availability but this effect is difficult to decouple from changes in community structure inferred from pigment analyses. Dissolved thiol concentrations also exceed typical metal concentrations in the subartic Pacific, supporting previous arguments that they may be important in metal speciation.     

Validation of a time-domain strip method to calculate the motions and loads on a fast monohull

The paper presents a comparison between experimental data and numerical results of the hydrodynamic coefficients and also of the wave induced motions and loads on a fast monohull model. The model with 4.52 m length was constructed in Fibre Reinforced Plastic (FRP), and made up of 4 segments connected by a backbone in order to measure sectional loads. The objective of the investigation was to assess the capability of a nonlinear time domain strip method to represent the nonlinear and also the forward speed effects on a displacement high speed vessel advancing in large amplitude waves. With this objective in mind the experimental program included forced oscillation tests in heaving and pitching, for a range of periods, three different amplitudes and several speeds of advance. In head regular waves comprehensive ranges of wave periods, wave steepness and speeds, were tested in order to measure heave, pitch and loads in three cross sections.

The numerical method assumes that the radiation and diffraction hydrodynamic forces are linear and the nonlinear contributions arise from the hydrostatics and Froude–Krilov forces and the effects of green water on deck. The assumption of linearity of the radiation forces is validated by comparing calculated hydrodynamic coefficients with experimental data for three different amplitudes of the forced oscillations. Both global coefficients and sectional coefficients are compared. The motions and loads in waves are compared in terms of first and higher harmonic amplitudes and also in terms of sagging and hogging peaks.     

On the use of grate-turbulators for tests of microstructural probes

Highly sensitive microstructural probes subjected to noise disturbances are free of these in laboratory tests. This leads necessarily to the working out of methods for complex testsin situ. The paper reports on the successful use for this purpose of a grate (net) suspended from a free-falling probe. The grate sets up turbulence with reproducible structure in the area of sensor location.UDK 551.46.085     

Bioerosion by chemosynthetic biological communities on Holocene submarine slide scars

Geomorphic, stratigraphic, and faunal observations of submarine slide scars that occur along the flanks of Monterey Canyon in 2.0–2.5 km water depths were made to identify the processes that continue to alter the surface of a submarine landslide scar after the initial slope failure. Deep-sea chemosynthetic biological communities and small caves are common on the sediment-free surfaces of the slide scars, especially along the headwall. The chemosynthetic organisms observed on slide scars in Monterey Canyon undergo a faunal succession based in part on their ability to maintain their access to the redox boundaries in the sediment on which they depend on as an energy source. By burrowing into the seafloor, these organisms are able to follow the retreating redox boundaries as geochemical re-equilibration occurs on the sole of the slide. As these organisms dig into the seafloor on the footwall, they often generate small caves and weaken the remaining seafloor. While chemosynthetic biological communities are typically used as indicators of fluid flow, these communities may be supported by methane and hydrogen sulfide that are diffusing out of the fresh seafloor exposed at the sole of the slide by the slope failure event. If so, these chemosynthetic biological communities may simply mark sites of recent seafloor exhumation, and are not reliable fluid seepage indicators.     

Seagrass landscape-scale changes in response to disturbance created by the dynamics of barrier-islands: A case study from Ria Formosa (Southern Portugal)

This study documents long-term changes of a Zostera noltii landscape induced by a natural cyclic event in a coastal lagoon. The barrier-islands forming this system are very dynamic with drifting movements controlling ecological patterns and processes occurring in this area. Changes in the areal extent of the Z. noltii meadows were assessed using historical aerial photographs from 1940, 1980, 1989, 1996 and 1998. Landscape indices such as total patch area (TA), mean patch size (MPS), number of patches (NP), mean shape coefficient of variation (CV) and landscape fractal dimension (D) were calculated for each year and related to an index of disturbance intensity. The spatial distribution of the Z. noltii meadows varied greatly during the studied period and changes observed were related to the disturbance created by the barrier-islands' spatial dynamics. After an artificial inlet relocation the Z. noltii area, number of patches, patch mean size and coefficient of variation decreased. The fractal dimension of the Z. noltii landscape increased by 50% showing that besides a decrease in total area, number of patches, and patch mean area, patch fragmentation was an important consequence of this anthropogenic disturbance.Seagrass natural distribution patterns changed in response to natural and human-induced activities. This study emphasizes the importance of the landscape approach and the historical perspective when studying seagrass changes and the importance of taking into consideration long-term changes in seagrass landscapes to avoid confusion between man-induced effects with natural cyclic events.     

Cyclic inelastic behavior and analytical modelling of pile–leg interaction in jacket type offshore platforms

Structures in locations susceptible to severe seismic disturbances should be designed properly in order to resist lateral forces induced by earthquake motions. Steel offshore platforms are some of those structures which are built to withstand environmental and accidental loads during oil exploitation operation. Particular attention is being paid to earthquake loads in seismic active areas because it directly influences the capacity of the offshore installations.

In this paper, a small-scaled planar platform has been modelled analytically using nonlinear finite element program, based on an experimental test, conducted simultaneously in order to assess the local and global behavior of pile–leg interaction in Jacket Type Offshore Platforms (JTOPs). A combination of nonlinear beam column elements and fatigue affected elements are used to capture the inelastic cyclic behavior of planar frame as accurately as possible. Results of analytical tests are to be compared with experiments and it is concluded that an analytical approach can be best used for modelling JTOPs with reasonable accuracy regardless of the type and scale of the structure. Moreover, a special study on joints has been carried out and the best model has been selected to simulate brittle behavior of joints resulting from heat affected zone.