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31.
Leaños-Castañeda O Gold-Bouchot G Van Der Kraak G Lister A Ceja-Moreno V Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342
The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT. 相似文献
32.
Jonsson H Sandnes KV Schiedek D Schneider R Grøsvik BE Goksøyr A 《Marine environmental research》2004,58(2-5):655-658
In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels. 相似文献
33.
双刺镰状水蚤Drepanopus bispinosus为南极沿海伯尔顿盐湖(Burton lake)中的越冬种类之一,其雌性性成熟大约于每年的初冬季节,在随后8个多月的生活史中,经历了一个盐度从33(5月份)到39(11月份)的环境变化.本文报道了该水蚤雌性成体对环境盐度适应的季节差异,动物在冬季的耐低盐能力较夏季强,从冬季到夏季,动物对盐度的适应范围明显地向高盐度方向偏移,这与环境盐度对动物的驯化有关.在低盐水中,动物的呼吸率增高,且夏季大于冬季,因而进一步证实了环境盐度的变化对动物盐度适应性的影响. 相似文献
34.
M.R.Li B.Z.Bian 《山东海洋学院学报》1986,16(4):215-216
From a stock culture of Cylindrotheca sp., non-motile colony-forming cells were observed and isolated.Colony-forming cells produced both single cells and colony-forming cells in liquid medla.the rate of single cell production varied among clones. 相似文献
35.
In order to assess in fish the maternal transfer of alkylphenolic compounds to the progeny, the identification and quantification of the labelled compounds present in oocytes and embryos was conducted after dietary exposure of mature female mosquitofish to 14C-4n-nonylphenol during vitellogenesis and embryogenesis respectively. Radioactivity found in bile and liver extracts accounted for 0.9-0.6 and 0.2-0.1% of ingested radioactivity for females exposed during vitellogenesis and embryogenesis respectively. The amount of extractable radioactivity present in oocytes and embryos was 0.19 and 0.07% of the ingested dose respectively. The radio-HPLC profiles obtained from bile, liver, oocyte and embryo extracts were similar. They showed the presence of 4n-NP-glucuronide as the major metabolite and traces of unchanged 4n-NP. The other metabolites corresponded to 8-hydroxynonylphenol, 9-(4-hydroxyphenyl)-nonanoic acid and para-hydroxybenzoic acid which is the final product of the alkyl chain oxidation. Our results indicate that exposure of ovoviviparous female fish to 4-NP during vitellogenesis and embryogenesis leads to the contamination of the progeny by 4-NP and its metabolites. 相似文献
36.
Geracitano LA Bocchetti R Monserrat JM Regoli F Bianchini A 《Marine environmental research》2004,58(1):1-17
Acute (4 days) and chronic (14 days) effects of copper were evaluated on the antioxidant defenses of Laeonereis acuta (Polychaeta) collected in unpolluted (UP) and polluted (P) sites. In the acute assay (125 and 250 micro g Cu/l) superoxide dismutase (SOD) and glutathione S-transferase (GST) activities did not change, whereas catalase (CAT) increased in worms from both the sites. Lipid peroxidation was higher in copper exposed worms from the P site. In the chronic assay (62.5 micro g Cu/l) polychaetes from the P site showed enhanced activities of SOD, GST and CAT and higher contents of metallothionein-like proteins and sulfhydrils compared to worms from UP. Differences in responses between polychaetes from UP and P sites suggest that organisms from the polluted site, P, are more susceptible to oxidative stress conditions. 相似文献
37.
巴巴多斯和新几内亚的海平面变化表明:在过去的15000a中曾有过两次大规模的冰川融水流.同其它环境变化(如北大西洋深水环流)相比,这两次融水流形成的时间显然有助于我们进一步了解从未次冰期到问冰期的气候变迁及新仙女木事件. 相似文献
38.
39.
Trends in emigration from Ireland over time are reviewed. "During the eighteenth and nineteenth centuries overseas migration to the United States and seasonal harvest migration to Britain were the main types of movement, but over the past 100 years the Irish have developed a special affinity for settling in British towns. Although the outflow was halted for a time during the 1970s, when return migration took over, the 1980s have seen a renewal of the exodus. This time, however, the character of the flow has changed from predominantly low-skill construction and factory workers to embrace better-educated emigrants, including many graduates. This shift reflects Ireland's changing position in the international market for labour." 相似文献
40.