印尼热泉菌Bacillus sp. BI-3产琼胶酶的发酵条件优化

采用响应面法对1株高产琼胶酶的印度尼西亚热泉菌Bacillus sp. BI-3的发酵条件进行优化。首先以温度、pH、不同碳源、不同氮源、不同金属离子及接种量作为唯一变量进行单因素实验, 筛选出对酶活有显著影响的单因素取值范围; 参考单因素实验结果, 采用Plackett-Burman实验设计筛选出影响酶活主要因素, 再利用Box-Behnken设计及响应面分析法进行回归分析以确定最佳发酵条件。结果表明, D-甘露糖、氯化锶和氯化钙与酶活存在着显著的相关性, 通过求解回归方程得到Bacillus sp. BI-3的发酵条件系统优化的结果为: 酵母粉0.3%、D-甘露糖0.66%、蛋白胨0.6%、氯化钙7.21mmol/L、氯化锶6.00mmol/L、氯化钠添加量6‰、培养温度为55℃、接种量1%、初始pH 6.0, 优化后发酵上清液的酶活达到6.0U/mL, 比优化前提高了2.4倍。     

Constitutive approach for rate‐sensitive anisotropic structured clays

This paper describes a constitutive approach to model the behavior of rate‐dependent anisotropic structured clay. Rate‐sensitivity is modeled using overstress viscoplasticity. Clay structure is treated as a viscous phenomenon whereby the viscosity of the undisturbed structured clay is initially very high and the viscosity degrades or decreases with plastic straining until the intrinsic or residual viscosity is reached. A microstructure tensor approach is used to make the structured viscosity anisotropic; whereas, the intrinsic viscosity is assumed to be isotropic. The behavior of the constitutive model is compared with the measured response of two clays (Gloucester and St. Vallier clay) from Eastern Canada during triaxial compression tests on specimens trimmed at different orientations to the vertical. The comparisons show that the constitutive framework is able to describe the anisotropic and rate‐sensitive response of both clays. The response of the model is also examined for the more general case of anisotropic consolidated triaxial compression and extension. Copyright © 2010 John Wiley & Sons, Ltd.     

高精度磁测考古的进展

概述了高精度磁测考古的对象,及其对测网布置、仪器和测量方式的选择以及日变观测方面的要求,并提出了进行高精度磁测考古的野外工作方法和技术要领。     

Geochemical characteristics of crude oils from Well Zheng-1 in the Junggar Basin, Xinjiang, China

Well Zheng-1 is located in the combined area of the central uplift and the north Tianshan piedmont depression in the Junggar Basin. Two oil-bearing beds are recognized at 4788–4797 m of the Lower Cretaceous Tugulu Formation (K1tg) and 4808.5–4812.5 m of the Lower Jurassic Sangonghe Formation (J1s). The geochemical characteristics of family composition, carbon isotopic composition, saturated hydrocarbons, sterane and terpane biomarkers and carotane of two crude oils are described in this paper. The results show that the geochemical characteristics of the two crude oils are basically similar to each other, indicating they were all derived mainly from the high mature, brine, algae-rich lake facies sediments. Oil-source correlation revealed that crude oils of the two beds were derived mainly from the source rocks of Permian and mixed by the oil derived from the source rocks of Jurassic and Triassic. This is consistent with the geological background with several sets of source rocks in the area studied.     

福建东南沿海第四纪盆地构造沉降

利用福建东南沿海地区城市活断层探测的研究资料,重新确认了福州、泉州和漳州盆地的断层组合与第四纪沉积。研究区内晚更新世“福州海侵”产生的炭质淤泥沉积现代高程的分析对比表明,晚更新世以来该区地壳发生不均匀隆升,而这些盆地相对于研究区内其他地区发生沉降。断层的静态位错理论研究认为,正断层深部活动引发地表的沉降,该区断裂的深部正断层活动是盆地沉降的重要因素。盆地的发展演化可划分为晚更新世以前的断陷阶段,和其间或其后的拗陷沉积阶段。     

Morphological analysis on the coherence of kHz QPOs

We take the recently published data of twin kHz quasi-period oscillations (QPOs) in neutron star (NS) low-mass X-ray binaries (LMXBs) as the samples, and investigate the morphology of the samples, which focuses on the quality factor, peak frequency of kHz QPOs, and try to infer their physical mechanism. We notice that: (1) The quality factors of upper kHz QPOs are low (2～20 in general) and increase with the kHz QPO peak frequencies for both Z and Atoll sources. (2) The distribution of quality factor versus frequency for the lower kHz QPOs are quite different between Z and Atoll sources. For most Z source samples, the quality factors of lower kHz QPOs are low (usually lower than 15) and rise steadily with the peak frequencies except for Sco X-1, which drop abruptly at the frequency of about 750 Hz. While for most Atoll sources, the quality factors of lower kHz QPOs are very high (from 2 to 200) and usually have a rising part, a maximum and an abrupt drop. (3) There are three Atoll sources (4U 1728-34, 4U 1636-53 and 4U 1608-52) of displaying very high quality factors for lower kHz QPOs. These three sources have been detected with the spin frequencies and sidebands, in which the source with higher spin frequency presents higher quality factor of lower kHz QPOs and lower difference between sideband frequency and lower kHz QPO frequency.     

J—A2500射频发生器及其维修

本文介绍J-A2500射频发生器的简单工作原理、调试方法和典型故障排除以及使用维修注意事项。     

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VP型宽频带倾斜仪映震能力分析

根据所掌握的仪器映震能力、震前(后)大震特殊捕捉现象,以及对相同地震不同仪器映震的对比,可以看出,VP型宽频带垂直摆倾斜仪具备前兆仪器捕捉(反映)地震孕育及地震发生的相关作用,能够正确、客观的作用于地震前兆数据产出,对地震预测研究具有深远影响.     

Strength Behavior of Shanghai Clayey Soil Reinforced with Wheat Straw Fibers

It is generally recognized that the low strength and high compressibility are the characteristics of soft soil. In addition to other techniques, reinforcement can also be used in increasing the strength and decreasing the deformation of this kind of soil. The results of an investigation into the effects of a natural fiber on the consolidation and shear strength behavior of Shanghai clayey soil reinforced with wheat straw fibers are presented in this paper. A series of one dimensional consolidation and triaxial tests were conducted on samples of unreinforced and reinforced Shanghai clayey soil with different percentages of randomly distributed wheat straw fibers. The results show that the preconsolidation pressure decreases and the coefficient of swelling and compression generally increase with increasing the fiber content until a optimum content value. Furthermore, the addition of wheat straw fiber leads to a significant increase in shear strength and friction angle of the natural soil and there is an optimum wheat fiber content that makes this increase maximal.