西太平洋深海海水中乙醛降解菌多样性及群落结构研究

As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

一种新型张力保护自动收放脐带缆绞车装置

介绍一种新型张力保护自动收放的脐带缆绞车装置的组成及特点。该装置克服了传统脐带缆绞车全人工操作模式所存在的效率低和安全性差等问题,实现了脐带缆绞车的自动收放和排缆,准确控制脐带缆张力,有效保护脐带缆的安全,大幅提高了潜水器等水下作业设备的工作效率和作业安全性。     

莫桑比克楠普拉省喀祖祖地区砂金成因及其对岩金指示意义

莫桑比克楠普拉省喀祖祖地区多条河流均有人工砂金开采活动.砂金分布在海拔300～450 m之间的石英砾石层,底部岩石主要为中元古代片麻状杂岩体.研究区为准平原-丘陵地貌,水动力条件受季节影响较大.砂金分布与河流不存在明显的耦合性,暗示了原生金矿化可能较分散.同时,研究区露头受第四纪覆盖严重,物化探效果不显著,砂金与重砂矿...     

试论中国城市化发展模式

结合城市化的新观念,提出了中国城市化发展新理论模式--中国城乡网络化发展模式.城乡网络化发展模式建立在区域综合协调发展基础之上,指的是在一定区域范围内城乡之间各种经济活动主体构成一个有序化的关联系统及其运行过程,并通过这个过程获得一种特有的网络组织功能效应.同时,还在此理论基础上针对我国东部、中部、西部地区各自的现实条件,分别对其实施城乡网络化发展进行了初步构想.     

基于MSC MARC的三维构造应力场数值模拟与裂缝预测——以宝浪油田宝北区块为例

MSC MARC的高度非线性有限元分析能力与强大的二次开发接口为复杂三维地质数值模型的建立与三维构造应力模拟与非线性分析提供了有力工具; 并可以通过二次开放子程序建立复杂的岩石本构与破裂准则。本文基于MSC MARC建立了新疆焉耆盆地宝浪油田宝北区块背斜构造三维有限元模型并进行了构造应力模拟分析和裂缝预测。裂缝预测结果与实际动态资料十分吻合。      

基于灰色关联分析预测法的腾冲硅藻土矿床资源评价

在矿产资源评价中,可以利用灰色理论的特点,来对目前只有少量信息的预测区进行评价和排序,确定预测靶区,指导找矿工作.应用该方法对云南西部腾冲地区的界头、团田、蛮朵、瑞滇、左所营5个工作区硅藻土资源进行排序,确定了腾冲硅藻土矿床开发下一步的工作方向,明确了矿床灰色关联分析预测法在矿产资源评价中有着重要的应用价值和独特的应用效果.     

冲绳海槽Jade热液活动区块状硫化物的铅同位素组成及其地质意义

新测行Ｊａｄｅ热液活动区中５件块状硫化物样品的铅同位素组成,具有较小的变化范围,表现出较均一的铅同位素组成特征。在Ｐｂ－Ｐｂ图解上,块状硫化物的铅同位素数据构成线形排列,与该区沉积物和蚀变火山岩的铅同位素组成一致,而与该区新鲜火山岩相比具较高的放射成因铅,证实了该区海底块状硫化物中的铅是由沉积物长英质火山岩来源铅共同构成的混合铅。不同热液活动区铅同位素组成对比研究表明,地质－构造环境的不同是导致各     

赣南东部银坑-青塘地区上施组硅质白云岩特征及其找矿意义

在赣南东部银坑－－青塘地区广泛分布晚元古代地层,近年普查找矿发现青白口纪上施组地层有两层典型硅质白云岩层,该白云岩层层位稳定,特征明显,并与本地区的铅锌矿床关系密切。本文主要从硅质白云岩层和层控铅锌矿床的一些基本特征入手,阐述了它们之间的相互关系,明确指出上施组硅质白云岩层对寻找富铅锌矿床具重要的意义。     

青山-横塘矿区铅锌矿床控矿断裂特征及找矿评价

黔西北铅锌成矿区海西期经历了北西向水城断陷的发育演化,沉积了巨厚的泥盆系、古炭系地层,形成区域内主要的矿化层位。青山－横塘矿区位于威水铅锌成矿带之水城中部矿田,区内构造发育,成矿条件有利,产出了青山、横塘等中小型矿床,从矿区内控矿断裂的同生活动特征分析,断陷盆地内ＮＷ 断裂的同生活动控制了矿带,活动较强烈地段控制了矿区,ＮＥ向断裂的同生活动控制了矿床。