褐菖鲉(Sebastiscus marmoratus)受精卵以及仔鱼期氨基酸与脂肪酸变化研究

为研究褐菖鲉(Sebastiscus marmoratus)发育早期氨基酸和脂肪酸的组成及变化规律,采用GC/MS法等方法分析了褐菖鲉的受精卵、初产仔鱼、前仔鱼期和后仔鱼期四个阶段的氨基酸和脂肪酸组成特点及其含量变化。结果表明:(1)总氨基酸含量从受精卵至初产仔鱼显著下降、前仔鱼期又显著回升、至后仔鱼期再次呈显著下降(P0.05);(2)总游离氨基酸量以初产仔鱼最高,是受精卵的10倍,各期的游离氨基酸占总氨基酸比值为1.4%—20.0%;(3)受精卵中以DHA、C16:0两者实际含量最高,分别为104.88 mg/g和68.74 mg/g,C18:1n-9和EPA次之,分别为41.23 mg/g和27.23 mg/g;(4)褐菖鲉内源性营养阶段被选择性消耗的主要脂肪酸依次为C16:0C18:1EPADHAARA,就脂肪酸的利用率而言MUFASFAPUFA;(5)褐菖鲉仔鱼在开口后的外源营养阶段对脂肪酸的利用率为PUFASFAMUFA,其中DHA相对EPA和ARA被选择性消耗。研究表明,褐菖鲉受精卵的游离氨基酸/总氨基酸比值符合海水鱼类沉性卵的特征,褐菖鲉在早期发育不同阶段对脂肪酸消耗具有不同的选择性,C16:1、C16:0、C18:0和C18:1是褐菖鲉早期发育的主要能源物质,在褐菖鲉开口饵料中添加一定水平的异亮氨酸、亮氨酸、酪氨酸等氨基酸及高度不饱和脂肪酸DHA等是十分必要的。     

温度、盐度和光照对浒苔生长和光合作用的影响

Intensive Pyropia aquaculture in the coast of southwestern Yellow Sea and its subsequent waste, including disposed Ulva prolifera, was speculated to be one of the major sources for the large-scale green tide proceeding in the Yellow Sea since 2007. It was, however, unclear how the detached U. prolifera responded and resumed growing after they detached from its original habitat. In this study, we investigated the growth and photosynthetic response of the detached U. prolifera to various temperature, salinity and irradiance in the laboratory. The photosynthetic rate of the detached U. prolifera was significantly higher at moderate temperature levels(14–27°C)and high salinity(26–32), with optimum at 23°C and 32. Both low(14°C) and highest temperature(40°C), as well as low salinity(8) had adverse effects on the photosynthesis. Compared with the other Ulva species, U. prolifera showed higher saturated irradiance and no significant photoinhibition at high irradiance, indicating the great tolerance of U. prolifera to the high irradiance. The dense branch and complex structure of floating mats could help protect the thalli and reduce photoinhibition in field. Furthermore, temperature exerted a stronger influence on the growth rate of the detached U. prolifera compared to salinity. Overall, the high growth rate of this detached U. prolifera(10.6%–16.7% d~(–1)) at a wide range of temperature(5–32°C) and salinity(14–32) implied its blooming tendency with fluctuated salinity and temperature during floating. The environmental parameters in the southwestern Yellow Sea at the beginning of green tide were coincident with the optimal conditions for the detached U. prolifera.     

放养密度对大杂交鲟生长性能的影响及生理应答机制

为探讨放养密度对鱼类生长及生理应答机制的影响规律和作用,作者以大规格大杂交鲟(达氏鳇(Huso dauricus)♀×施氏鲟(Acipenser schrenckii)♂)为实验材料,研究了平均初始体质量约为243 g/尾,放养密度分别为6(SD1组)、9(SD2组)、12(SD3组)、15 kg/m3(SD4组)条件下,不同放养密度处理70 d后的实验鱼生长性能变化及生理应答机制。结果显示,放养密度对大杂交鲟肥满度影响不显著,SD2组鱼类具有最大的特定生长率和生长效率,随着放养密度增加,日增质量显著降低(P0.05),特定生长率和生长效率下降。测定了血液甲状腺素(T4)、三碘甲腺原氨酸(T3)和皮质醇水平变化,发现放养密度能引起大杂交鲟3个血液生理指标发生显著改变;随着养殖时间推移,T3和皮质醇浓度显著升高,T4浓度显著下降(P0.05)。这些结果说明神经内分泌活动的变化引起大杂交鲟血液生理指标变化,进而影响实验鱼生长性能。因此,在该养殖条件下推荐的养殖密度为9 kg/m3。     

西菲律宾海2万年以来浮游有孔虫壳体重量变化

为探讨2万年以来浮游有孔虫的壳体重量变化及其影响因素,对取自西菲律宾海MD06-3052岩芯的浮游有孔虫表层种Globigerinoides ruber进行壳体重量分析,基于氧同位素年龄模式,获得了2万年以来有孔虫传统壳体重量(WMS)和标准化壳体重量(WSN)变化特征。对比两种壳体重量指标,发现在本海区标准化壳体重量指标更为准确。结果表明,2万年以来,浮游有孔虫WSN具有不断变轻的趋势,整体表现为冰期时壳体重量较重,而全新世时壳体重量相对较轻的特征。末次冰盛期(Last Glacial Maximum,LGM)以来浮游有孔虫WSN变化曲线同大气CO2浓度变化曲线具有较好的相似性,推测在该海区控制壳体重量的主要因素为与全球大气CO2相关的表层海水的CO32–浓度。     

天津市滨海新区芦苇湿地恢复适宜性评价

利用综合评价模型,结合地理信息系统技术的空间分析功能,以土地利用、土壤类型、坡度、降雨量和人类干扰活动作为评价因子,对天津滨海新区芦苇湿地恢复进行了适宜性评价。结果表明,较适宜开展湿地恢复的比例为21.46%,一般适宜比例为21.87%,不适宜比例占56.68%,其中较适宜湿地恢复的区域集中分布在北大港湿地自然保护区周边、独流减河下游、北塘入海口以及沿海滩涂,海河流域两侧也有零星分布。在适宜性评价基础上,对未来芦苇湿地恢复与建设的重点区域进行了成效预评估,芦苇对TC、TN、TP的年吸收通量约为1.3935×104、0.0258×104、0.0017×104t,能有效地减少入海排污压力和污染物对近岸海域环境质量的影响。研究结论可为天津湿地治理与生态恢复、土地利用结构调整、景观优化等提供理论依据和科学参考。     

Beyond dual-porosity modeling for the simulation of complex flow mechanisms in shale reservoirs

The state of the art of modeling fluid flow in shale reservoirs is dominated by dual-porosity models which divide the reservoirs into matrix blocks that significantly contribute to fluid storage and fracture networks which principally control flow capacity. However, recent extensive microscopic studies reveal that there exist massive micro- and nano-pore systems in shale matrices. Because of this, the actual flow mechanisms in shale reservoirs are considerably more complex than can be simulated by the conventional dual-porosity models and Darcy’s law. Therefore, a model capturing multiple pore scales and flow can provide a better understanding of the complex flow mechanisms occurring in these reservoirs. This paper presents a micro-scale multiple-porosity model for fluid flow in shale reservoirs by capturing the dynamics occurring in three porosity systems: inorganic matter, organic matter (mainly kerogen), and natural fractures. Inorganic and organic portions of shale matrix are treated as sub-blocks with different attributes, such as wettability and pore structures. In kerogen, gas desorption and diffusion are the dominant physics. Since the flow regimes are sensitive to pore size, the effects of nano-pores and micro-pores in kerogen are incorporated into the simulator. The multiple-porosity model is built upon a unique tool for simulating general multiple-porosity systems in which several porosity systems may be tied to each other through arbitrary connectivities. This new model allows us to better understand complex flow mechanisms and eventually is extended into the reservoir scale through upscaling techniques. Sensitivity studies on the contributions of the different flow mechanisms and kerogen properties give some insight as to their importance. Results also include a comparison of the conventional dual-porosity treatment and show that significant differences in fluid distributions and dynamics are obtained with the improved multiple-porosity simulation.     

唐山市海岛岸滩旅游环境质量评价

海滩作为宝贵的滨海旅游资源,其质量问题越来越受到关注,海滩质量评价不仅能为海滩使用者提供帮助,也为提高旅游海滩质量提供指导方法。唐山市砂质岸滩资源丰富,岸线总长度约50 km,几乎全部位于海岛,砂质细腻。参考国内外质量评价标准,选取25个评价因子(自然因子12个,社会因子13个)对唐山市海滩进行评价,综合来看,海滩质量以祥云岛最好,龙岛次之,其余海岛均表现为一般。     

南海某导管架海洋平台倒塌分析

针对在役老龄导管架平台进行倒塌计算分析,确定极限承载力进而评估老龄导管架的安全裕度。采用非线性有限元方法,考虑平台的波流载荷及桩-土的非线性相互作用,利用SACS软件建立导管架整体三维有限元计算分析模型,并用逐步加载的方式,对南海某导管架平台进行了全过程非线性倒塌分析。计算分析表明,该导管架平台极限强度很高,具有较大的安全裕度;导管架倒塌过程呈逐步破坏形式,先是撑杆屈服,造成局部结构破坏,然后是钢桩发生屈服,降低结构承载力,最后节点逐步失效,造成结构倒塌。揭示了导管架平台结构失效倒塌的机理,给出了倒塌分析的可行方法和步骤。     

Factors driving riverbed scouring and sedimentation in the Bayangaole to Toudaoguai reaches of the Upper Yellow River

The Inner Mongolia reaches of the Yellow River face problems of severe sedimentation caused by a variety of complex factors. The sedimentation process in those reaches has been characterized using the sediment balance method, and the key factors affecting the process have been analyzed using the correlation analysis method. The results show that during the period 1952–2012 the Bayangaole (Bayan Gol) to Toudaoguai reaches in Inner Mongolia have undergone successive processes of accumulative sedimentation, then relative balance, and then accumulative sedimentation once again. The total annual sedimentation is 12.0341×10⁸ m³, of which accumulations from July to October account for 95.1% and the reaches from Sanhuhekou to Toudaoguai account for 98.5%. The main factor affecting scouring and sedimentation of the Bayangaole to Sanhuhekou reaches is the combined water and sediment condition. The critical conditions for equilibrium are an incoming sediment coefficient < 0.007 kg·s·m^–6 and a flow discharge > 700 m³·s^–1. The main factor affecting scouring and sedimentation of the Sanhuhekou to Toudaoguai reaches is the incoming sediment from the tributaries on the south bank and the combined water and sediment condition of the main stream. The critical conditions of the main stream for maintaining equilibrium status are a flow discharge of the main stream exceeding 800 m³·s^–1 and a comprehensive incoming sediment coefficient < 0.005 kg·s·m^–6. The incoming sediment from the tributaries has little impact on the main stream when the annual sediment load is less than 0.1×10⁸ t. The incoming sediment coefficient of the main stream and the incoming sediment from the tributaries both play vital roles in the riverbed evolution of the Inner Mongolia reaches, but the latter contributes the most.     

崇明岛东滩湿地芦苇光合作用对土壤水盐因子的响应

2015年3~10月,在崇明岛东滩湿地设置7块采样地,以不同水盐条件下生长的芦苇(Phragmites australis)为材料,应用便携式光合系统分析仪(Li-6400),对芦苇的光响应特征进行实地测定,探讨芦苇光合过程对干旱和盐胁迫的响应机理。结果表明:1各月采样日的水位与土壤电导率显著负相关(n=420,p0.01),适当增加土壤水位,有利于降低土壤电导率,从而缓解植物盐胁迫伤害;28月21日的芦苇叶片净光合速率、蒸腾速率和气孔导度与水位显著正相关(n=42,p0.01),而与土壤电导率显著负相关(n=42,p0.01);胞间CO2浓度与水位和土壤电导率不相关。当水位高于-50 cm,净光合速率随着水位下降而降低,非气孔因素可能是净光合速率下降的主要原因;当水位低于-50 cm时,土壤电导率高于10 m S/cm,叶片蒸腾速率快速降低,水分利用效率不断提高,芦苇叶片以较低的净光合速率来适应干旱胁迫和盐胁迫;芦苇叶片通过降低最大净光合速率、暗呼吸速率、光补偿点和光饱和点等光合生理特征来适应干旱胁迫和盐胁迫;芦苇叶片净光合速率和最大净光合速率对土壤水盐因子具有高度相关性,可以作为土壤水盐胁迫程度的反应指标。因此,在芦苇快速生长阶段,合理调控水位,对芦苇光合作用具有重要意义。