太平洋鳕鱼脑中硫苷脂的分离纯化和分析

以太平洋鳕鱼为原料,从鳕鱼脑中分离硫苷脂,确立了提取及纯化的条件,并对硫苷脂的纯度和分子种进行分析。首先,采用氯仿/甲醇(2∶1,v/v)提取总脂。然后,依次采用氯仿/甲醇/水(7∶3∶0.3,v/v/v)洗脱硅胶柱层析,含0.2mol/L乙酸铵的氯仿/甲醇/水(30∶60∶8,v/v/v)洗脱DEAE Sephadex-A25离子交换柱层析,40%甲醇脱盐和100%甲醇洗脱反相C8柱层析,获得硫苷脂纯品。最后,利用500YMC Diol液相色谱柱(3.0mm×250mm,5μm)分离,以正己烷/异丙醇(70∶30,v/v),异丙醇/水/甲酸/氨水(100∶13∶1∶0.14,v/v/v/v)为流动相梯度洗脱,采用负电喷雾电离(ESI)和母离子扫描模式,用液相色谱-串联质谱(LC-MS/MS)法分析了鳕鱼脑硫苷脂的纯度和分子种组成,并比较了其与哺乳动物脑中硫苷脂分子种组成的异同。结果表明,本实验制得的鳕鱼脑硫苷脂纯度为90.74%,与哺乳动物类似,鳕鱼脑硫苷脂的长链碱基以鞘氨醇为主,主要分子种为d18∶1-C24∶1,但脂肪酸的羟基化程度略低,且含少量独特的分子种,如d18∶1-22∶1和d18∶2-25∶2。     

Evaluation of the biological toxicity of fluorine in Antarctic krill

Antarctic krill is a potentially nutritious food source for humans, but fluorine(F) toxicity is a matter of concern. To evaluate the toxicity of F in Antarctic krill, 30 Wistar rats were divided into three groups with different dietary regimens: a control group, a krill treatment group(150 mg·kg-1 F), and a sodium fluoride(NaF) treatment group(150 mg·kg-1 F). After three months, F concentrations in feces, plasma, and bone were determined, and the degree of dental and skeletal fluorosis was assessed. The F concentrations in plasma and bone from the krill treatment group were 0.167 0±0.020 4 mg.L-1 and 2 709.8±301.9 mg·kg-1, respectively, compared with 0.043 8±0.005 5 mg·L-1 and 442.4±60.7 mg·kg-1, respectively, in samples from the control group. Concentrations of F in plasma and bone in the krill treatment group were higher than in the control group, but lower than in the NaF treatment group. The degree of dental fluorosis in the krill treatment group was moderate, compared with severe in the NaF treatment group and normal in the control group. The degree of skeletal fluorosis did not change significantly in any group. These results showed that the toxicity of F in Antarctic krill was lower than for an equivalent concentration of F in NaF, but it was toxic for rats consuming krill in large quantities. To conclude, we discuss possible reasons for the reduced toxicity of F in Antarctic krill. The present study provides a direct toxicological reference for the consideration of Antarctic krill for human consumption.     

大数据时代的人类移动性研究

人类个体/群体移动特征是多学科共同关注的研究主题。移动定位、无线通讯和移动互联网技术的快速发展使得获取大规模、长时间序列、精细时空粒度的个体移动轨迹和相互作用定量化成为可能。同时,地理信息科学、统计物理学、复杂网络科学和计算机科学等多学科交叉也为人类移动性研究的定量化提供了有力支撑。本文首先系统总结了大数据时代开展人类移动性研究的多源异构数据基础和多学科研究方法,然后将人类移动性研究归纳为面向人和面向地理空间两大方向。面向人的研究侧重探索人类移动特性的统计规律,并建立模型解释相应的动力学机制,或分析人类活动模式,并预测出行或活动;面向地理空间的研究侧重从地理视角分析人类群体在地理空间中的移动,探索宏观活动和地理空间的交互特征。围绕这两大方向,本文评述了人类移动性的研究进展和存在问题,认为人类移动性研究在数据稀疏性、数据偏斜影响与处理、多源异构数据挖掘、机器学习方法等方面依然面临挑战,对多学科研究方法的交叉与融合提出了更高要求。     

Modeling seasonal variations of subsurface chlorophyll maximum in South China Sea

In the South China Sea(SCS), the subsurface chlorophyll maximum(SCM) is frequently observed while the mechanisms of SCM occurrence have not been well understood. In this study, a 1-D physical-biochemical coupled model was used to study the seasonal variations of vertical profiles of chlorophyll-a(Chl-a) in the SCS. Three parameters(i.e., SCM layer(SCML) depth, thickness, and intensity) were defined to characterize the vertical distribution of Chl-a in SCML and were obtained by fitting the vertical profile of Chl-a in the subsurface layer using a Gaussian function. The seasonal variations of SCMs are reproduced reasonably well compared to the observations. The annual averages of SCML depth, thickness, and intensity are 75 ± 10 m, 31 ± 6.7 m, and 0.37 ± 0.11 mg m-3, respectively. A thick, close to surface SCML together with a higher intensity occurs during the northeastern monsoon. Both the SCML thickness and intensity are sensitive to the changes of surface wind speed in winter and summer, but the surface wind speed exerts a minor influence on the SCML depth; for example, double strengthening of the southwestern monsoon in summer can lead to the thickening of SCML by 46%, the intensity decreasing by 30%, and the shoaling by 6%. This is because part of nutrients are pumped from the upper nutricline to the surface mixed layer by strong vertical mixing. Increasing initial nutrient concentrations by two times will increase the intensity of SCML by over 80% in winter and spring. The sensitivity analysis indicates that light attenuation is critical to the three parameters of SCM. Decreasing background light attenuation by 20% extends the euphotic zone, makes SCML deeper(~20%) and thicker(12% – 41%), and increases the intensity by over 16%. Overall, the depth of SCML is mainly controlled by light attenuation, and the SCML thickness and intensity are closely associated with wind and initial nitrate concentration in the SCS.     

Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.     

广西寒露风开始期短期气候预测方法研究

利用1951-2013年广西90个气象观测站气温资料、国家气候中心74项指数和美国National Oceanic and Atmospheric Administration（NOAA）的Climate Prediction Center（CPC）60项指数以及海温和陆地雪盖资料、美国国家冰雪研究中心（NSIDC）的两极海冰资料,使用相关分析方法得到广西寒露风开始期气候影响因子,利用逐步回归和神经网络方法进行寒露风开始期的预测。结果表明：寒露风开始期与前一年9-10月北极海冰面积、当年3月南极海冰面积、前一年6月欧亚雪盖、当年5月北美雪盖、北半球雪盖的相关显著。与前一年9月北半球极涡面积指数、前一年10月亚洲区极涡面积指数、前一年3月热带印度洋海温偶极子等指数相关显著。粒子群-神经网络方法预测误差低于逐步回归方法,预报能力有明显提高。     

凡纳滨对虾(Litopenaeus vannamei)Runt转录因子的基因克隆及其功能分析

Runt结构域转录因子家族(Runx)在哺乳动物体内存在三种基因型,分别起着造血,骨生成和控制上皮细胞增殖的作用。本文应用反转录和c DNA末端快速扩增(RACE)技术,首次在凡纳滨对虾(Litopenaeus vannamei)中克隆并测序了一种Runt(lvrunt)基因全长。lvrunt c DNA全长1754bp,含有1个663bp长的开放阅读框,编码221个氨基酸,理论分子量为23.6k Da,具有Runt-family结构域。对凡纳滨对虾鳃、肠、肝胰腺、类淋巴、心脏、肌肉组织和血淋巴lvrunt的转录特征进行分析,结果显示该基因几乎特异性地在血淋巴细胞中表达,而在其他组织中表达较低。肌肉注射重组造血激素蛋白(r AST)或白斑综合征病毒(WSSV)粗提液均可显著提高lvrunt在凡纳滨对虾体内的转录表达。上述结果提示,lvrunt主要在血细胞发挥作用,可能作为造血激素的下游基因起到调节血细胞增殖和分化的作用,并在受到病毒感染后,参与提升血细胞数量或者促进血细胞分化的过程。     

始新统油气系统:泰国湾东北部油气勘探新层系

泰国湾区域经历了前裂谷期、裂谷期、裂后期的构造演化阶段,形成了多个裂谷盆地。泰国湾区域东北部在渐新世经历了一次明显构造反转,较泰国湾区域大部分地区强烈。通过对比区内钻井,结合地震解释,对该区的沉积特征和构造演化进行了分析,认为这次反转构造导致了反转构造带上构造、沉积特征与邻区有较大的不同。由于这次反转构造,泰国湾东北部在新层系发育新类型的油气系统,即深部的始新统油气系统:烃源岩为中始新统湖相泥岩,储层为上始新统-渐新统三角洲相砂岩,盖层为下中新统三角洲前缘相泥岩和上中新统以上的海相泥岩。该油气成藏系统已被钻井钻遇油气显示,是本区有效油气成藏系统。     

Spatial distribution pattern of seafloor hydrothermal vents to the southeastern Kueishan Tao offshore Taiwan Island

Investigations of the diffusion activities both within and outside the seafloor hydrothermal vents, as well as related mineral genesis, have been one of the key focuses of ocean biogeochemistry studies. Many hydrothermal vents are distributed close to the southern Okinawa Trough on the less-than-30-m deep shallow seafloor off Kueishan Tao, northeast of Taiwan Island. Investigations of temperature, pH and Eh at four depths of hydrothermal plume were carried out near Kueishan Tao at the white(24.83°N, 121.96°E) and yellow(24.83°N, 121.96°E) vents. An 87 h of temperature time series observation-undertaken near the white vent showed that tide is the main factor affecting the background environment. Based on the observed data, 3-dimensional sliced diffusion fields were obtained and analyzed. It was concluded that the plume diffused mainly from north to south due to ebb tide. The yellow vent's plume could effect as far as the white vent surface. From the temperature diffusion field, the vortices of the plume were observed. The Eh negative abnormality was a better indicator to search for hydrothermal plumes and locate hydrothermal vents than high temperature and low pH abnormalities.     

东海西部陆架海域水团的季节特征分析

On the basis of the CTD data and the modeling results in the winter and summer of 2009, the seasonal characteristics of the water masses in the western East China Sea shelf area were analyzed using a cluster analysis method. The results show that the distributions and temperature-salinity characteristics of the water masses in the study area are of distinct seasonal difference. In the western East China Sea shelf area, there are three water masses during winter, i.e., continental coastal water（CCW）, Taiwan Warm Current surface water（TWCSW） and Yellow Sea mixing water（YSMW）, but four ones during summer, i.e., the CCW, the TWCSW, Taiwan Warm Current deep water（TWCDW） and the YSMW. Of all, the CCW, the TWCSW and the TWCDW are all dominant water masses. The CCW, primarily characterized by a low salinity, has lower temperature, higher salinity and smaller spatial extent in winter than in summer. The TWCSW is warmer, fresher and smaller in summer than in winter, and it originates mostly from the Kuroshio surface water（KSW） northeast of Taiwan, China and less from the Taiwan Strait water during winter, but it consists of the strait water and the KSW during summer. The TWCDW is characterized by a low temperature and a high salinity, and originates completely in the Kuroshio subsurface water northeast of Taiwan.