Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

贺兰山小松山基性-超基性杂岩体岩石地球化学特征

贺兰山小松山岩体的岩石地球化学研究表明,基性岩的Mg^#为0．71～0．76,接近原生岩浆的值,超基性岩的Mg^#为0．80～0．89,具有岩浆早期堆晶的特征,稀土总量∑REE为（13．03～82．66）×10^-6;δ（Eu）为1．08～1．61,具弱正异常;（La／Yb）N为2．49～5．24,（Ce／Yb）N为3．01～4．97,配分曲线表现为轻稀土相对富集的右倾型。微量元素配分曲线图表现为大离子亲石元素富集。岩石地球化学数据综合分析证明,该岩体产于板内拉张环境,岩体形成过程中经历了岩浆分离结晶作用,后期发生了同化混染,岩体具有寻找铬-铜-镍型岩浆矿床的潜力。     

鄂尔多斯盆地查布水源地地下水水化学特征及其影响因素

根据94件水化学样品的分析结果,对查布水源地地下水水化学特征及其空间分布规律进行了研究,并结合含水层沉积环境及补径条件探讨了地下水化学成分的影响因素,以期为水源地水质保护提供依据。结果表明：地下水水化学类型呈现明显的水平分带性,东部地下水中阴离子以HCO3^-为主,地下水水质一般较好,矿化度多小于1g／L;西部地下水中阴离子以SO4^2-、Cl^-为主,水质变差,矿化度明显增高;研究区硝酸盐污染比较明显,F^-局部超标,矿化度超标也比较明显;水质受含水层的沉积环境和地下水补径条件及人畜活动的影响明显。水源地开采后,其影响会加大,因此应制定合理的水源地保护方案。     

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淄博市淄川区矿产资源现状及远景规划

淄川区为淄博市辖区之一,位于市境中部,南邻博山区,西接章丘县,北与周村、张店、临淄3区相连,东傍青州市,东南与临朐、沂源2县接壤。东西长49km,南北宽42km,总面积999.07km2。淄川区辖4个街道办事处,14个镇,3个乡,人口为67.29万人。境内自东向西有淄河、孝妇河、范阳河、青杨河4条河流。该区工业较发达,区内的支柱产业为建材、化工、纺织、制药、陶瓷。全区交通以铁路、公路为主,有张博路、胶王路、湖南路等国道和省道,交通非常便利。1矿产资源现状1.1矿产资源的基本特点淄川区在地层区划上属于华北地层区、鲁西地层分区、泰安地层小区。在…     

基于洪峰模数的山洪灾害雨量预警指标研究

山洪灾害预警是防御山洪的重要非工程措施,雨量预警指标是山洪灾害预警的关键。目前的雨量预警指标计算方法对水文气象资料条件以及模型建模率定都有很高的要求,并不适用于基层防汛人员。因此,本文基于全国山洪灾害调查评价成果数据,提出了一种运用洪峰模数计算雨量预警指标的简便、易用的方法。该方法以小流域洪水计算推理公式为基础,将公式中流量与流域面积的比值用洪峰模数表示,得到基于洪峰模数的临界雨量估算公式,并考虑流域土壤含水量等因素,分析临界雨量变化阈值,最终得到雨量预警指标。本文以云南省绥江县双河小流域为例,计算结果显示不同时段（1 h、3 h、6 h）净雨量和预警时段呈线性关系。降雨损失计算中洼地蓄水和植被截留在不同时段相同,土壤下渗在不同的时段不相同。在此基础上,计算不同土壤含水量条件下,不同时段的雨量预警指标。最后,对临界流量、降雨损失和预警指标进行了合理性分析,结果显示预警指标和调查评价结果及实测降雨都比较接近,计算的预警指标合理。本研究为基层山洪灾害预警提供了一种快速、便捷的预警指标计算方法,为预警指标计算提供技术支持。     

Localization and dynamic expression of a 27.8 kDa receptor protein for lymphocystis disease virus infection in sea bass (<Emphasis Type="Italic">Lateolabrax japonicus</Emphasis>) tissues

Lymphocystis disease virus (LCDV) infects target cells by attaching to a 27.8 kDa receptor (27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies (MAbs) have been developed. However, the 27.8R existence in tissues of sea bass (Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass.     

气象因素对倾斜仪观测干扰特征分析

采用常熟台水管仪与垂直摆2009～2015年观测数据,研究了气压、降雨、气温、洞温等因素对地倾斜观测的影响特征。结果表明,降雨对地倾斜观测数据干扰最为显著,表现为NS分量发生南倾,EW分量发生西倾,量级上NS分量受干扰程度比EW分量大,垂直摆受干扰程度比水管仪大;气压变化影响表现为短周期扰动,并导致日值曲线出现短时间畸变;气温及洞温变化影响观测值出现明显的年变规律和年变幅变化,对日观测值影响不明显。     

长江三峡库区地震地形变监测研究

三峡地壳形变监测网络结合空间大地测量(GPS、InSAR)和传统大地测量的优点，在1998年以来的三峡工程建设期间和2003年水库首次蓄水中获取了大量监测成果。结果显示库区地壳运动的基本图像：垂直形变较为显著，主要来自因蓄水荷载而产生的形变，水平形变不明显．库区主要断层的活动性在蓄水期间有明显加强迹象。水准测量反映从1998年首期观测至2003年10月首次蓄水后的库区垂直位移．沉降范围较大；GPS给出了2003年蓄水前后库区的垂直位移，形变的范围主要集中在水库近岸区域。水准测量与GPS观测反应的垂直位移的量级基本一致，沉降的最大幅度在3～4cm左右。水准监测、GPS观测与重力监测结果有一定的对应性。从地壳形变监测与研究结果分析，近期负荷改变而诱发中强地震的可能性不大。     

面向城市平原地区暴雨积涝汇水区分级划分的方法研究

汇水区划分是分布式水文模型计算的基础。针对现有方法使用DEM在平原城市地区划分的汇水区不符合实际地形情况,本文提出了一种面向城市平原地区分级划分汇水区的技术方法。该方法从城市用地分类角度出发将城市分为中心城区和郊区,依据城市排水主干水系进行汇水区一级划分,将影响中心城区和郊区的不同径流因子分别融入DEM中,利用细化的DEM进行二级汇水区划分;在此基础上,根据实际汇流情况,结合Voronoi图,对中心城区进行三级划分,最后通过GIS技术进行修正。该方法既结合了传统DEM生成子流域的算法,又融入了城市区域地物地貌特点,能更好地满足城市地区的需要。选择上海市嘉定区西北部地区为实验样区,利用该方法进行汇水区划分比较表明,其对于城市平原地区具有很好的适用性。