MALBAC技术扩增微生物群落基因组的效率评估

环境样品的低生物量是微生物宏基因组学研究面临的首要挑战,通过基因组扩增技术来满足高通量测序对DNA样品量的需求是最常用的解决策略。MALBAC(Multiple Annealing and Looping Based Amplification Cycles)基因组扩增试剂盒最初为扩增和研究哺乳动物的单细胞基因组而研发。本文中,我们通过人工构建的微生物群落来检测该试剂盒在微生物宏基因组扩增方面的效率和应用可行性。结果表明,每个标准反应中,10 pg的DNA模板量足以满足MALBAC试剂盒对样品扩增的需要。每个标准反应DNA模板用量为10和100 pg时,所扩增DNA样品的基因组覆盖度与原始未扩增样品表现出高度的一致性,证明MALBAC试剂盒扩增效果的高度稳定性和一致性。常用的GenomePlex全基因组扩增试剂盒使我们可以在每个标准反应DNA模板量为100 pg的条件下扩增获得足够的DNA样品,但是结果表明该参照试剂盒无法有效的实现对群落中低丰度细菌菌株基因组的线性扩增。对于MALBAC试剂盒和参照试剂盒而言,在扩增高GC含量的微生物物种基因组DNA方面效率低下。我们的实验结果表明MALBAC试剂盒在高效扩增环境样品宏基因组DNA方面的可行性,但对该试剂盒在扩增环境样品中高GC含量微生物物种方面的适用性存在疑虑。     

A synthetic aperture radar sea surface distribution estimation by n-order Bézier curve and its application in ship detection

To dates,most ship detection approaches for single-pol synthetic aperture radar(SAR) imagery try to ensure a constant false-alarm rate(CFAR).A high performance ship detector relies on two key components:an accurate estimation to a sea surface distribution and a fine designed CFAR algorithm.First,a novel nonparametric sea surface distribution estimation method is developed based on n-order Bézier curve.To estimate the sea surface distribution using n-order Bézier curve,an explicit analytical solution is derived based on a least square optimization,and the optimal selection also is presented to two essential parameters,the order n of Bézier curve and the number m of sample points.Next,to validate the ship detection performance of the estimated sea surface distribution,the estimated sea surface distribution by n-order Bézier curve is combined with a cell averaging CFAR(CA-CFAR).To eliminate the possible interfering ship targets in background window,an improved automatic censoring method is applied.Comprehensive experiments prove that in terms of sea surface estimation performance,the proposed method is as good as a traditional nonparametric Parzen window kernel method,and in most cases,outperforms two widely used parametric methods,K and G0 models.In terms of computation speed,a major advantage of the proposed estimation method is the time consuming only depended on the number m of sample points while independent of imagery size,which makes it can achieve a significant speed improvement to the Parzen window kernel method,and in some cases,it is even faster than two parametric methods.In terms of ship detection performance,the experiments show that the ship detector which constructed by the proposed sea surface distribution model and the given CA-CFAR algorithm has wide adaptability to different SAR sensors,resolutions and sea surface homogeneities and obtains a leading performance on the test dataset.     

消油剂处理120#燃料油对海水青鳉(Oryzias melastigma)胚胎抗氧化酶活性影响的研究

以海水青鳉(Oryzias melastigma)胚胎为研究对象,比较了120#燃料油分散液(water-accommodated fractions,WAFs)与乳化液(biologically enhanced water-accommodated fractions,BE-WAFs)的急性毒性效应,并研究了不同浓度(40、100、250 mg/L)下WAFs、BE-WAFs对胚胎内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)活性的影响。结果表明:在受到石油烃的氧化胁迫后,海水青鳉胚胎内3种抗氧化酶活性变化明显。随着石油烃浓度的升高和暴露时间的延长,3种酶表现出程度不同的诱导效应和抑制效应。其中受石油烃污染影响最为明显的为SOD酶;而GST酶则对消油剂单独暴露表现较为敏感。实验证明,海水青鳉体内SOD酶活性对石油烃污染反应最为敏感,适合作为监测石油烃污染程度的生物标志物。     

基于动态极值理论和Copula函数的极端海平面高度预测建模

全球气候变化背景下,海平面上升是一个潜在的重大风险,为防范气候灾害,应对极端气象海洋事件,需客观、定量地对未来极端海平面变化进行科学预测。为此,基于Copula函数和动态极值分析理论,综合考虑平均海平面变化(包括垂直陆地运动和基准的局地变化)与潮、涌、浪等其他气候变化的增水对极端海平面高度的影响,采用DREAM方法改进Bayes推断对动态极值模型的参数空间估计问题,提出一种新的模型对未来极端海平面高度变化进行预测,旨在改进传统模型存在的不确定性问题,并运用该模型对气候变化背景下厦门地区未来35年的海平面变化情景进行了模型应用和实验模拟。     

国际上的政治地理学研究进展与启示————对《Political Geography》杂志2005-2015年载文的分析

通过对国际上的政治地理学旗舰性杂志《Political Geography》2005-2015年期间391篇成果的分析,总结出国际政治地理学研究的主要研究议题为:边境与边界,环境政治,空间与尺度,领土和主权以及安全问题,选举地理,社会和文化政治,其中空间与尺度,领土和主权以及安全问题,社会和文化政治几个方向研究成果占较大比例,而其他三个方向则相对较少.通过研究方法分析发现,国际政治地理学主要采用定性分析的方法,尤其是民族志和文本分析的方法,定量分析的使用存在一定的局限性.最后,从该杂志的研究对象和研究机构两个方面的分析归纳出:欧美主导的发达国家(尤其是英国和美国)对世界政治地理学话题的学术霸权与世界南,北差异发展具有一定的相关性.在中国综合国力日渐增强,参与国际事务能力不断提高以及作为新兴的地缘政治力量崛起的社会历史背景下,政治地理学经世致用之能亟待被展现.因此,中国政治地理学发展作为发展中国家的声音势必将越来越洪亮.     

基于水边线等高的海岸带卫星影像定位方法

针对海岸带和海岛(礁)稀少地面控制区域控制条件不足和定位精度较低等情况,将水边线等高条件与有理函数模型(RFM)相结合,充分利用海岸带卫星影像的特点,将水边线等高点引入平差,提出了一种基于水边线等高的海岸带稀少地面控制卫星影像定位方法。实验结果表明,在同样稀少地面控制情况下,该方法与未加入等高控制条件的海岸带卫星影像定位方法相比,高程定位精度有了明显的提高;在离海岛较近的大陆岸边布设控制点,利用本文方法同样可以在海岛获取较高的定位精度。这对于稀少地面控制条件下海岸带、海岛(礁)地理信息的精确获取,具有重要意义。     

Geochemical and Petrological Studies on the Early Carboniferous Sidingheishan Mafic–Ultramafic Iintrusion in the Southern Margin of the Central Asian Orogenic Belt, NW China

The Sidingheishan mafic-ultramafic intrusion is located in the eastern part of the Northern Tianshan Mountain, along the southern margin of the Central Asian Orogenic Belt in northern Xinjiang autonomous region of China. The Sidingheishan intrusion is mainly composed of wehrlite, olivine websterite, olivine gabbro, gabbro and hornblende gabbro. At least two pulses of magma were involved in the formation of the intrusion. The first pulse of magma produced an olivine-free unit and the second pulse produced an olivine-bearing unit. The magmas intruded the Devonian granites and granodiorites.An age of 351.4±5.8 Ma(Early Carboniferous) for the Sidingheishan intrusion has been determined by U-Pb SHRIMP analysis of zircon grains separated from the olivine gabbro unit. A U-Pb age of 359.2±6.4 Ma from the gabbro unit has been obtained by LA-ICP-MS. Olivine of the Sidingheishan intrusion reaches 82.52 mole% Fo and 1414 ppm Ni. On the basis of olivine-liquid equilibria, it has been calculated that the MgO and FeO included in the parental magma of a wehrlite sample were approximately10.43 wt% and 13.14 wt%, respectively. The Sidingheishan intrusive rocks are characterized by moderate enrichments in Th and Sm, slight enrichments in light REE, and depletions in Nb, Ta, Zr and Hf. The ε_(Nd)(t) values in the rock units vary from +6.70 to +9.64, and initial ~(87)Sr/~(86)Sr ratios range between 0.7035 and0.7042. Initial ~(206)Pb/~(204)Pb, ~(207)Pb/~(204)Pb and ~(208)Pb/~(204)Pb values fall in the ranges of 17.23-17.91,15.45-15.54 and 37.54-38.09 respectively. These characteristics are collectively similar to the Heishan intrusion and the Early Carboniferous subduction related volcanic rocks in the Santanghu Basin, North Tianshan and Beishan area. The low(La/Gd)_(PM) values between 0.26 and 1.77 indicate that the magma of the Sidingheishan intrusion was most likely derived from a depleted spinel-peridotite mantle.(Th/Nb)_(PM)ratios from 0.59 to 20.25 indicate contamination of the parental magma in the upper crust.Crystallization modeling methods suggest that the parental magma of the Sidingheishan intrusion was generated by flush melting of the asthenosphere and subsequently there was about 10 vol%contamination from a granitic melt. This was followed by about 5 vol% assimilation of upper crustal rocks. Thus, the high-Mg basaltic parental magma of Sidingheishan intrusion is interpreted to have formed from partial melting of the asthenosphere during the break-off of a subducted slab.     

Comparative Study of Hydrogen and Carbon Isotopic Composition of Gases Generated from the Pyrolysis of a Peat under Saltwater and Freshwater Conditions

To understand the influence of the diagenetic water medium on the isotopic compositions of thermogenic coalbed gas, both hydrous and anhydrous closed-system pyrolyses were performed at temperatures of 250°C to 650°C on an herbaceous marsh peat. Compared to the results of anhydrous pyrolysis, the hydrocarbon gases generated from hydrous pyrolyses have very different hydrogen isotopic compositions. However, the carbon isotopic compositions of the hydrocarbon gases became only slightly heavier in hydrous pyrolysis, compared to that from anhydrous pyrolysis. With the progress of thermal evolution from peat to a more advanced thermal maturity of vitrinite reflectance values (Ro) of 5.5% during the pyrolysis, the difference in the average δD value increased from 52‰ to 64‰ between the hydrous pyrolysis with saltwater and anhydrous pyrolysis and increased from 18‰ to 29‰ between the hydrous pyrolysis with freshwater and anhydrous pyrolysis, respectively. The difference in the average δ13C value was only 1‰–2‰ between the hydrous and anhydrous pyrolysis. The relationships between the δD values of the generated hydrocarbon gases and Ro values as well as among δD values of the hydrocarbon gas species are established. The close relationships among these parameters suggest that the water medium had a significant effect on the hydrogen isotopic composition and a minimal effect on the carbon isotopic composition of the hydrocarbon gases. The results of these pyrolyses may provide information for the understanding of the genesis of coalbed gas from herbaceous marsh material with the participation of different diagenetic water media.     

乌尉高速公路边坡稳定性综合评价

针对乌尉(乌鲁木齐至尉犁)高速公路地处高寒、高海拔、高地震烈度区,地质环境条件复杂的特点,提出一种基于多判据的边坡稳定性模糊综合评价方法。首先,分析研究三高地区公路沿线工程地质条件、边坡岩体结构特征及其变形破坏模式。在此基础上,以TSMR评价体系为框架,考虑地震荷载对边坡稳定性的影响,引入地震作用影响系数β,建立了适用于“三高”地区公路边坡岩体质量分级体系THRSMR;基于边坡可靠性分析理论,归纳总结Monte Carlo法的操作步骤,并借助GEO-SLOPE软件,给出各研究边坡的可靠度指标与失效概率;最后,将定性评价、极限平衡分析、可靠度分析和数值计算的结果分别作为基础指标,采用模糊综合评价方法对每一边坡的边坡稳定性,给出进一步量化结论。其结果对类似地区高速公路边坡稳定性评价具有一定的参考价值。     

基于振动台实验的典型滑坡单元体震动滑移特征发现

针对典型滑坡滑动单元在地震过程中的滑移特征,制作了典型振动台物理模型,通过输入不同频率、峰值和持时的正弦地震波,开展了一系列的实验研究工作,得了一些比较有意义的新成果:（1）在输入地震波强度足够大时会出现瞬时沿滑带向上的滑移行为,存在向上和向下两个滑移屈服加速度;（2）在研究分析中,不可以坡底输入的地震加速度值而应以滑带处局部加速度值作为判别滑移与否和滑移量大小的计算数据;（3）滑体沿滑带产生的永久滑移总量随地震波持时增加呈阶梯波状增长;（4）不同时刻任一周期的振动滑移特征相对独立,且每一地震波周期时间内具有几乎相同的周期性滑移特征,总体滑移量是无数周期内滑移量的累加;（5）在每一地震波周期时间内,滑体向上和向下的滑移特征受控于相应向上和向下屈服加速度,其向上和向下滑移矢量和构成每一周期时间内的永久位移。